Journal of
Soil Science and Environmental Management

  • Abbreviation: J. Soil Sci. Environ. Manage.
  • Language: English
  • ISSN: 2141-2391
  • DOI: 10.5897/JSSEM
  • Start Year: 2010
  • Published Articles: 311

Full Length Research Paper

Effects of Phanerochaete chrysosporium on biologic activity of soil amended with olive mill wastewaters

Ali Mekki*, Fathi Aloui, Abdelhafidh Dhouib and Sami Sayadi      
Laboratory of Bioprocesses, Center of Biotechnology of Sfax, AUF (PER-LBP), BP: 1177, 3018 Sfax, Tunisia.
Email: [email protected]

  •  Accepted: 30 October 2011
  •  Published: 31 January 2012

Abstract

Effects of the untreated olive mill wastewater (UOMW) and the bioaugmented olive mill wastewater (BOMW) with the fungus Phanerochaete chrysosporium (Pchrysosporium) for the amendment of soil were investigated. Results showed that UOMW inhibited the soil respirometric activity, while BOMW exhibited significantly higher respiration levels compared to the unamended and the UOMW amended soils. The ratio of C-CO2/Ctot decreased from 6.7 in the unamended soil to 6.34, to 2.74 and to 1.6 in soils amended consecutively with 50, 100 and 200 m3 ha-1 of UOMW. Based on the first results, the UOMW dose of 50 m3 ha-1 showed the elevated C-CO2/Ctot ratio in comparison with the two other doses (100 and 200 m3 ha-1) so that it was chosen to test the effects of its bioaugmentation with Pchrysosporium on the soil biodegradation activities. The fungus Pchrysosporium was added at three different forms; spores suspension (106 spores g-1), mycelium form (cultivated in liquid medium) and colony form (mycelium fragments from solid medium).The soil amended with 50 m3 ha-1 of BOMW showed higher C-CO2/Ctot ratio in comparison with control soil (unamended) and with soil amended with 50 m3 ha-1 of UOMW. The C-CO2/Ctot ratio increased from 6.34 in the soil amended by 50 m3 ha-1 of UOMW and 6.7 in the control soil to 27 (nearly 4 fold) in soil amended by BOMW with the spores of Pchrysosporium, to 18.3 (nearly 2.7 fold) in soil amended by BOMW with Pchrysosporium at mycelium form and to 17.5 (nearly 2.6 fold) in soil amended by BOMW with Pchrysosporium at colony form. These investigations illustrated that the quantity of 50 m3 ha-1 of olive mill wastewater bioaugmented byPchrysosporium at spores form is very advantageous choice for the stimulation of the respirometric activities of soil autochthonous microflora.

 

Key words: olive mill wastewater; bioaugmentation; Phanerochaete chrysosporium; soil; respirometric activities.