Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 351

Full Length Research Paper

Seroepidemiological investigation of foot-and-mouth disease virus serotypes in cattle around Lake Mburo National Park in South-western Uganda

Frank Norbert Mwiine
  • Frank Norbert Mwiine
  • Department of Veterinary Medicine, Faculty of Veterinary Medicine, Makerere University, Box 7062, Kampala-Uganda.Ministry of Agriculture, Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda.
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Chrisostom Ayebazibwe
  • Chrisostom Ayebazibwe
  • Ministry of Agriculture, Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda.
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Soren Alexandersen
  • Soren Alexandersen
  • National Veterinary Institute, Technical University of Denmark, Lindholm, DK 4771, Kalvehave, Denmark.Current address: National Centre for Foreign Animal Diseases, 1015 Arlington Street, Winnipeg MBR3E 3M4, Government of Canada.
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William Olaho-Mukani
  • William Olaho-Mukani
  • Ministry of Agriculture, Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda.
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A. R. Okurut Ademun
  • A. R. Okurut Ademun
  • Ministry of Agriculture, Animal Industry and Fisheries, P. O. Box 513, Entebbe, Uganda.
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Kirsten Tjornehoj
  • Kirsten Tjornehoj
  • National Veterinary Institute, Technical University of Denmark, Lindholm, DK 4771, Kalvehave, Denmark.
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  • Article Number - 1776E601433
  • Vol.2(4), pp. 46-54, November 2010
  •  Accepted: 13 July 2010
  •  Published: 30 November 2010

Abstract

Foot-and-mouth disease (FMD) outbreaks in cattle occur annually in Uganda. In this study the authors investigated antibodies against FMD virus (FMDV) in cattle in surrounding areas of Lake Mburo National Park in South-western Uganda. Two hundred and eleven serum samples from 23 cattle herds were examined for the presence of antibodies against FMDV non-structural proteins and structural proteins using Ceditest® FMDV-NS and Ceditest® FMDV type O (Cedi Diagnostics BV, Lelystad, The Netherlands). Furthermore, serotype-specific antibodies against the seven serotypes of FMDV were determined using in-house serotype-specific Solid Phase Blocking ELISAs (SPBE). Of the sera tested, 42.7% (90/211) were positive in the ELISA for antibodies against non-structural proteins, while 75.4% (159/211) had antibodies against the structural proteins of FMDV serotype O. Titres of ≥ 1:160 of serotype-specific antibodies in SPBEs were identified in 61% (19/31), 33% (5/15), 6%7 (20/30), 37% (10/27) and 12% (4/33) of the investigated samples for serotypes O, A, SAT 1, SAT 2 and SAT 3, respectively. This study indicates that most of the FMD outbreaks in the cattle herds in the investigated area were probably caused by FMDV serotype O, A and/ or SAT-serotype(s). It also shows that the usage of non-purified, multivalent vaccines in Uganda obscures the serological diagnosis of FMDV outbreaks, and that the sampling strategy needs to be improved. Finally, it emphasizes the importance of isolation and characterization of FMD viruses responsible for outbreaks in the area.

 

Key words: Foot-and-mouth-disease, antibodies, cattle, Uganda.