Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 351

Full Length Research Paper

Prevalence and possible risk factors for caprine paratuberculosis in intensive dairy production units in Guanajuato, Mexico

Claudia Celic Guzmán Ruiz
  • Claudia Celic Guzmán Ruiz
  • Universidad de Guanajuato, División Ciencias de la Vida, Campus Irapuato –Salamanca. Exhacienda, El Copal Km 9, Carretera Irapuato-Silao, CP 36820. Irapuato, Guanajuato, Mexico.
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Marco Antonio Santillán Flores
  • Marco Antonio Santillán Flores
  • Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias-CENID Microbiología Carretera México -Toluca km. 15.5, Colonia Palo Alto, CP. 04110. Delegación Cuajimalpa, México.
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Dionicio Córdova López
  • Dionicio Córdova López
  • Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias-CENID Microbiología Carretera México -Toluca km. 15.5, Colonia Palo Alto, CP. 04110. Delegación Cuajimalpa, México.
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  •  Received: 13 June 2016
  •  Accepted: 26 September 2016
  •  Published: 30 November 2016

Abstract

In order to determine the prevalence of caprine paratuberculosis and the possible risk factors associated a cross-sectional epidemiological study (February to June, 2009) was carried out in dairy goat intensive production units in Guanajuto, Mexico. Blood (n=821) and fecal (n=240) were sampled from goats (older than one year of age) were randomly selected from thirteen dairy goat intensive production units. Serum samples were analyzed using immunodiffusion serological test (AGIT) and fecal samples were used for confirmatory diagnosis by IS 900 Nested PCR. Analysis of results was carried out with STATA 7® software. Paratuberculosis prevalence in the studied population was 9.87% (range 1 to 32%), females had 10.25% and male 6.24%; for body condition were 14.02% emaciated, 7.94% thin and 8.7% good. By Nested PCR analysis detected 64 (26.67%) fecal samples as positive. Risk factor analysis indicated that alpine breed had a OR=2.1 (95%, CI 0.76 to 7.65), females in theirs second parturition showed OR= 2.94 (95%,CI 1.04 to 8.3), from three to fifth parturition had OR= 5.88 (95%, CI 4.3 to14.7) and with more than six parturitions had an OR=7 (95%, CI 2.79 to 18.21)., Animals that presented pasty to liquid feces had OR=2.10 (95%, CI 0.84 to5.25). The results in this study suggest that there is an increased risk for paratuberculosis as the number of parturitions increases and is also related to the consistency and handling of feces in the flocks.

Key words: Paratuberculosis, caprine, epidemiology, nested, polymerase chain reaction (PCR), immunodiffusion, intensive production.