Ethno-veterinary medicine is the use of traditional knowledge, theory, and skill to mitigate animal diseases. The study conducted to document veterinary use of medicinal herbs in Assosa Zone, Ethiopia from September 2022 to July 2023. Data collected between individual based field’s interviews using semi-structured questionnaire. Most of the respondents were males and with age group of 40 to 60 years old. Most of the respondents were illiterate by educational status and Muslims by religion. Around 68.6% of respondents acquire their traditional knowledge from family and most respondents prepared remedies from plants 73 (71.6%). In this investigation, 71 plant species from five districts identified. Those plants used against 68 types of animal diseases. Those plants distributed across 43 families. Data from this study revealed that the large number of plant species was found in Solanaceae and Fabaceae family followed by Combreataceae and Asteraceae. Most respondents explained that the medicinal plants were found from forest (42.25%) followed by both backyard and forest (35.21%), and only Backyard (21.13%). Majorities of respondents explained that the root part of the medicinal plant use for medicinal value (25.35%), followed by leaf and seed (22.54%), and bark (8.45%). The study revealed that the local healers in the districts have been heavily dependent on traditional animal health care for the treatment of variety of animal diseases. Thus, it should recommend that Plants specifically important for treatment of diseases should be test on phytochemical screening, antioxidant activity and antibacterial effects. In addition, conservational activities for medicinal plants and governmental encouragements of traditional healers should conduct.

Keywords: Ethno-veterinary, plant, respondent, medicinal plant