Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 125

Full Length Research Paper

Evaluation of antifungal activity of snake venoms from the Amazon forest

Marcelo dos Santos Neves1, Diego Rayan Teixeira de Sousa3, Maria do Perpétuo Socorro Borges Carriço Ferreira2, Maria Zeli Moreira Frota2, João Vicente Braga Souza3* and Jorge Luis López Lozano1
1Universidade do Estado do Amazonas/Fundação de Medicina Tropical- Dr Heitor Vieira Dourado, Brazil. 2Federal University of Amazonas / UFAM, Brazil. 3Mycology Laboratory of the National Institute of Amazonian Research / INPA, Brazil.
Email: [email protected]

  •  Received: 04 November 2014
  •  Accepted: 30 March 2015
  •  Published: 30 May 2015

Abstract

In recent years, many antimicrobial peptides have been found in the venoms of animals from different sources and have been intensively studied to elucidate their ability to inhibit the growth of potential pathogenic microorganisms. The aim of this study was to characterize and evaluate the in vitro antifungal activity of crude venom from two amazonian snakes: Bothrops atrox and Crotalus durissus ruruima. The molecular profile of representative proteins from the venom samples was obtained by reversed-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Fungal inhibition was investigated by microdilution assays against two Candida albicans strains. Based on the chromatography and electrophoresis analyses, the venom from B. atrox and C. durissus ruruima were characterized. In addition, the venoms (400 µg/mL) were not able to cause significant inhibition (> 50%) of the growth of C. albicans KL-07, at only 9.09% (200 µg/mL) and 7.88% (400 µg/mL), respectively, and neither presented any influence on the growth of strain C. albicans ATCC 36232.
 
Key words: Antifungal, activity, snake and Amazon.