Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 125

Full Length Research Paper

Influence of thermal and osmotic pressure on the growth and viability of Candida shehatae var shehatae

Sadia Afrin
  • Sadia Afrin
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh.
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Jannatul Sumaiya Promee
  • Jannatul Sumaiya Promee
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh.
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Meher Nahid
  • Meher Nahid
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh.
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Mohammed A. Satter
  • Mohammed A. Satter
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh.
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Mohammad Amirul Haque
  • Mohammad Amirul Haque
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh.
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Ashish Kumar Sarker
  • Ashish Kumar Sarker
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh.
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Mohammad Nazrul Islam Bhuiyan
  • Mohammad Nazrul Islam Bhuiyan
  • Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh.
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  •  Received: 23 September 2020
  •  Accepted: 17 June 2021
  •  Published: 31 July 2021

Abstract

Environmental stresses can bring abrupt changes in microbes and it can affect its growth with a number of visible modifications. Although some studies emphasize on the bacterial and fungal cellular response against acute heat and mitochondrial substrate oxidative stresses, in case of yeast very few researches were conducted. Currently, Saccharomyces cerevisiae is only known yeast about their responses against different stresses. The present study has been performed to observe how the growth and budding pattern of Candida shehatae var shehatae will be influenced by high temperature (40-45°C), as well as the impact of osmotic stresses on yeast cell. To find out the critical growth temperature of C. shehatae var shehatae growth was monitored at 40 and 45°C for 72 h. Osmotic stress tolerance was observed at 32.5°C in diverse dextrose (0.04 g/l (1X), 0.12 g/l (3X), 0.2 g/l (5X), 0.28 g/l (7X), 0.36 g/l (9X)) and sucrose, that is 0.02 g/l (1X), 0.06 g/l (3X), 0.1 g/l (5X), 0.14 g/l (7X) , 0.18 g/l (9X) concentrations. The result was monitored at 600 nm at different time intervals. The viability of strains was analyzed by determination of CFU/ml. The optimal growth pattern was observed at 32.5°C and cells lose their cultivability at 45°C. Cell growth and budding were observed under high dextrose and sucrose concentrations and suppressed at 45°C. Results of the current study indicated that the critical growth temperature is 45°C and under sugar concentrations stress stimulant was also shown at that temperatures.

 

Keywords: Candida shehatae var shehatae, dextrose, heat stress, critical growth, osmotic stress, morphological pattern.