Research in Pharmaceutical Biotechnology

  • Abbreviation: Res. Pharm. Biotech.
  • Language: English
  • ISSN: 2141-2324
  • DOI: 10.5897/RPB
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Antifungal activity of aqueous and ethanolic extracts of Picralima nitida seeds on Aspergillus flavus, Candida albicans and Microsporum canis

Peace Ubulom
  • Peace Ubulom
  • Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria.
  • Google Scholar
Ekaete Akpabio
  • Ekaete Akpabio
  • Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria.
  • Google Scholar
Chinweizu Ejikeme udobi
  • Chinweizu Ejikeme udobi
  • College of Science and Technology, Kaduna Polytechnic, Kaduna, Nigeria.
  • Google Scholar
Ruth Mbon
  • Ruth Mbon
  • Faculty of Pharmacy, University of Uyo, Akwa Ibom State, Nigeria.
  • Google Scholar


  • Article Number - 714DB8C471
  • Vol.3(5), pp. 57-60, May 2011
  •  Accepted: 31 March 2011
  •  Published: 30 May 2011

Abstract

 

Aqueous and ethanolic extracts of Picralima nitida seeds were tested for their antifungal activities using Aspergillus flavus, Candida albicans and Microsporum canis as test organisms. Phytochemical analysis revealed the presence of some plant metabolites which have been reported to have antimicrobial effects. Assays were performed using extract concentrations of 25, 50, 100 and 200 mg/ml and the agar well diffusion technique was employed. Results obtained, revealed a significant difference (P<0.05) in inhibition zone diameter between A. flavus, and C. albicans and between C. albicans and M. canis. However,there was no significant difference (P>0.05) in inhibition zone diameter between A. flavus andM. canis.  With the aqueous seed extract, a significant difference (P<0.05) in inhibition zone diameter was observed between A. flavus and C. albicans and between A. flavus and M. canis but there was no significant difference (P>0.05) in inhibition zone diameter between C. albicans and M. canis. The inhibitory activity of the ethanolic extract on each test organism was compared with that of the aqueous extract and in all cases the observed difference was significant (P<0.05). These findings indicate the potentials of the seeds of P. nitida as panacea for some fungal infections.

 

Key words: Antifungal activity, aqueous extract, ethanolic extract, Picralima nitida seeds.