Preliminary phytochemical screening of Striga hermonthica was carried out to assess the chemical contents and biological activity of callus comparing to that of intact plant (upper and underground parts). The results show the presence of terpenes, tannins, coumarins, cardiac glycosides, flavonoids, saponins, anthracenosides and alkaloids. Further, ethanol extracts analysis-using thin layer chromatography (TLC) revealed differences in chemical constituents between calli, and different parts of the plant with five fractions in callus, three fractions in upper-parts and two fractions in underground-parts based on solvent systems. Antimicrobial assay of S. hermonthica extracts revealed various activities against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans using agar well-diffusion method. The richest extract with phytochemical constituents and most effective one was the ethanol extracts of the different parts.
Key words: Striga hermonthica, phytochemical screening, thin layer chromatography, callus, flavonoids, antimicrobial assay.
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