Research in Pharmaceutical Biotechnology

  • Abbreviation: Res. Pharm. Biotech.
  • Language: English
  • ISSN: 2141-2324
  • DOI: 10.5897/RPB
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Phytochemical and biological study of Striga hermonthica (Del.) Benth callus and intact plant

Faisal Hammad Mekky Koua
  • Faisal Hammad Mekky Koua
  • Department of Biochemistry, Faculty of Science and Technology, Al Neelain University, P. O. Box 12702, Al Baladya St. Khartoum, Sudan.
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Hind Ahmed Babiker
  • Hind Ahmed Babiker
  • Department of Biochemistry, Faculty of Science and Technology, Al Neelain University, P. O. Box 12702, Al Baladya St. Khartoum, Sudan.
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Asim Halfawi
  • Asim Halfawi
  • Deparment of Pharmacy, University of Medical Sciences and Technology, P. O. Box: 12810, Khartoum, Sudan.
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Rabie Osman Ibrahim
  • Rabie Osman Ibrahim
  • Department of Biochemistry, Faculty of Science and Technology, Al Neelain University, P. O. Box 12702, Al Baladya St. Khartoum, Sudan.
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Fatima Misbah Abbas
  • Fatima Misbah Abbas
  • Commission for Biotechnology and Genetic Engineering, National Center for Research P. O. Box 2404 Khartoum, Sudan.
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Eisa Ibrahim Elgaali
  • Eisa Ibrahim Elgaali
  • Commission for Biotechnology and Genetic Engineering, National Center for Research P. O. Box 2404 Khartoum, Sudan.
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Mutasim Mohamed Khlafallah
  • Mutasim Mohamed Khlafallah
  • Commission for Biotechnology and Genetic Engineering, National Center for Research P. O. Box 2404 Khartoum, Sudan.
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  • Article Number - A3F5981479
  • Vol.3(7), pp. 85-92, July 2011
  •  Accepted: 08 June 2011
  •  Published: 31 July 2011

Abstract

 

Preliminary phytochemical screening of Striga hermonthica was carried out to assess the chemical contents and biological activity of callus comparing to that of intact plant (upper and underground parts). The results show the presence of terpenes, tannins, coumarins, cardiac glycosides, flavonoids, saponins, anthracenosides and alkaloids. Further, ethanol extracts analysis-using thin layer chromatography (TLC) revealed differences in chemical constituents between calli, and different parts of the plant with five fractions in callus, three fractions in upper-parts and two fractions in underground-parts based on solvent systems. Antimicrobial assay of S. hermonthica extracts revealed various activities against Staphylococcus aureusPseudomonas aeruginosaEscherichia coli and Candida albicans using agar well-diffusion method. The richest extract with phytochemical constituents and most effective one was the ethanol extracts of the different parts.

 

Key words: Striga hermonthica, phytochemical screening, thin layer chromatography, callus, flavonoids, antimicrobial assay.