Experiments were conducted to document optimum ways of producing sturdy and suitable planting materials of Bryophyllum pinnatum, Cordia millenii, Enantia cholrantha and Celtis durandii found in Southern Nigeria. The plants are used in the treatment of various diseases such as earache, fever, malaria, typhoid, jaundice, liver problem and menstrual pains. Forest top soil was observed to be the best growth medium. The stem cuttings of B. pinnatum did not root in all the experiments while leaf rooted along the serrated margin. Within 30 days, 18 seedlings were produced in forest topsoil as against 10 produced in river sand. Significant differences were observed in response to coconut milk treatment under the various time durations. 24 new plants were produced from the leaves soaked for 24 h as against 3 from the leaves of B. pinnatum soaked for 1 min from forest topsoil. Lower values from river sand were observed. Mycorrhizal association was observed to exist. Seedlings inoculated with mycorrhizal soil had a mean total value of 90 cm in height growth against the control where 30 cm was obtained. Significant stem rooting was noted in response to coconut milk treatment except with B. pinnatum in which zero values were obtained. 60% of the stem cuttings rooted was observed in E. cholorantha, and 50% in C. millenii and C. durandii with 24 h treatment with coconut milk. The determination of the phytochemical constituents revealed that the plants contain saponin, alkaloids, tanins, inulins, potassium mallate, ascorbic and organic acids. The plants are used in the treatment of various diseases such as earache, fever, malaria, typhoid, jaundice, liver problem and menstrual pains.
Key words: Conservation, Bryophyllum pinnatum, Cordia millenii, Enantia chlorantha, Celtis durandii.
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