Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Isolation and molecular sub typing of Salmonella enterica from chicken, beef and street foods in Malaysia

Shabnam Modarressi1,2 and Kwai Lin Thong1,2*
1Microbiology Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. 2Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Email: [email protected]

  •  Accepted: 30 August 2010
  •  Published: 18 September 2010

Abstract

This study was conducted to determine and identify Salmonella isolated from chicken meat, beef and street food (poultry and beef products) and the genetic diversity of Salmonella by pulsed-field gel electrophoresis (PFGE). Eighty-eight non-repeat Salmonella isolates from 300 food samples were isolated and characterized using conventional culture, biochemical and serological methods and confirmation of Salmonella was determined by polymerase chain reaction(PCR) using specific primers. Among the 88 Salmonella isolates, 11 serovars [Corvallis (n = 33), Typhimurium (n = 18), Hadar (n = 7), Enteritidis (n = 5),Weltevreden (n = 5), Agona (n = 5), Newport (n = 4), Albany (n = 3), Istanbul (n = 2), Emek (n = 1) and Wandsworth (n = 1)] were identified. All the 88 Salmonellaisolates were typable by PFGE with 61 distinct pulsed-field profiles. The cluster analysis indicates that 61 different pulsotypes were separated into groups of identical serovars based on their PFGE profiles. The isolation of various serovars of Salmonella from different sources indicates the presence and distribution ofSalmonella in raw meats and street foods. Knowledge of variability of subtypes of different serovars and sources of Salmonella may provide valuable added information for research, risk management, and public health strategies.

 

Key words: Salmonella enterica, food, polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE).