Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2755

Full Length Research Paper

Rosmarinic acid production from transformed root cultures of Nepeta cataria L.

  Sook Young Lee1, Chung Yeol Lee2, Seok Hyun Eom3, Yong Kyoung Kim4, Nam Il Park4 and Sang Un Park4*        
  1Research Center for Oral Disease Regulation of the Aged, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Korea. 2Department of Plant Bioscience, College of Natural Resourece and Life Science,Pusan National University, Miryang, Gyeongsangnam-do 627-706, Korea. 3Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, 1 Seocheon-Dong, Giheung-Gu, Yongin, Gyeonggido 446-701, Korea. 4Department of Crop Science, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon 305-754, Korea.
Email: [email protected]

  •  Accepted: 29 March 2010
  •  Published: 31 May 2010

Abstract

 

Rosmarinic acid, a natural polyphenol antioxidant carboxylic acid, is an important secondary metabolite in Nepeta cataria, a member of the mint family (Labiatae) and has antioxidant, anti-inflammatory and antimicrobial activities. To investigate,in vitro production of rosmarinic acid, hairy root cultures of N. cataria were established by infecting leaf and stem explants with Agrobacterium rhizogenesR1000, and tested the growth and rosmarinic acid production of these cultures. Hairy roots were cultured in Murashige and Skoog liquid medium and maximum growth (11.2 g dry wt/l) was attained after 15 days of culture, at which time the content of rosmarinic acid was 13.6 mg/g dry wt. These results demonstrate that hairy root culture of N. cataria is a valuable alternative approach for the production of rosmarinic acid.
 
Key words: Agrobacterium rhizogenes, catnip, hairy root, Nepeta cataria L., rosmarinic acid.