Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Review

Effects of dexamethasone on expression of heat shock protein 70 in the liver of rats with traumatic shock

Donglin Luo1, Baohua Liu1, Jihong Zhou1, Xiaobao Ren2*, Renping Xiong1, Tao Li1 and Guangyan Li1
1Department of General Surgery, Institute of Battle Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China. 2Department of Emergency, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China.
Email: [email protected]

  •  Accepted: 28 July 2010
  •  Published: 18 September 2010

Abstract

It is still unknown whether glucocorticoid has certain effect on the HSP70 expression and structure, and function of the liver after traumatic shock, so in the present study, we investigated the effects of dexamethasone on heat shock protein 70 (HSP70) expression, liver structure and function in rats with traumatic shock. However, a hemorrhagic shock model was established by inducing bilateral femoral fractures in male Wistar rats. 20 mg/Kg dexamethasone was given by intraperitoneal injection, while shock occurred. A total of 72 adult male Wistar rats were randomly divided into 3 groups: Control group (n = 12), trauma group (n = 30), and dexamethasone followed by trauma shock group (n = 30). Hepatic HSP70, hepatic function, TNF–α and IL-6 were dynamically obtained at 0.5, 2, 4, 6 and 8 h after trauma. Liver histopathological changes were also observed. The expression of HSP70 in hepatic tissue was detected by western blot. In rats with trauma shock, HSP70 expression increased significantly compared with the control group, and peaked at 6 h after trauma. Hepatic TNF–α and IL-6, as well as serum alanine transferase (ALT) and total bilirubin (TB) were also elevated significantly, and peaked at 6 and 8 h, respectively after trauma. Light microscopy revealed hepatic congestion with infiltration of inflammatory cells into hepatic sinusoid at 8 h in the trauma shock group. After dexamethasone treatment, expression of HSP70 increased significantly compared with the trauma shock group. Levels of TNF–α and IL-6, as well as ALT and TB also lowered after trauma, and hepatic congestion as well as hepatic degeneration was ameliorated, with minimal inflammatory infiltrates noted in the sinusoids. Dexamethasone may enhance expression of HSP70 in liver in a rodent model of hemorrhagic shock after trauma, and ameliorate hepatic secondary lesion. It is indicated that dexamethasone is correlated to HSP70 in the course of liver protection after traumatic shock.

 

Key words: Trauma, hemorrhagic shock, liver, heat shock protein, dexamethasone.