Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2752

Full Length Research Paper

Antigenotoxic effects of Indian mustard Brassica juncea (L.) Czern aqueous seeds extract against mercury (Hg) induced genotoxicity

  Sonia Sharma and Adarsh Pal Vig*        
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar-143005 Punjab, India.
Email: [email protected]

  •  Accepted: 26 March 2012
  •  Published: 30 April 2012

Abstract

 

The present study evaluates the antigenotoxic effects of B. juncea (L.) Czern.(Indian mustard) aqueous seed extract on the Allium cepa L. (common onion) root tip cells. The experiment was carried at three stages: (1) onion bulbs were treated with 0.75 ppm mercury for 3 h, and then treated with different concentrations (0.1, 0.25, 0.50, 0.75, and 1.0%) of aqueous extract for another 3 h. (2) onion bulbs were treated with different concentrations of extract for 3 h, and then treated with 0.75 ppm concentration of mercury. (3) Onion bulbs were treated with different concentrations of extract and 0.75 ppm concentration of mercury simultaneously for 3 h. Increase in root growth and mitotic index and decrease in chromosomal aberrations were observed in root tip cells treated with aqueous extract of seeds, after before and simultaneously with mercury treatment as compared to control. The dose dependent effects suggest that aqueous extract of Indian mustard has antigenotoxic potential against mercury induced genotoxicity.

 

Key words: Indian mustard, antigenotoxic, chromosomal aberrations, mercury.