Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2729

Full Length Research Paper

Honey activity on the proliferation and migration of oral squamous cell carcinoma: In vitro and bioinformatic analysis

Eliane Macedo Sobrinho Santos
  • Eliane Macedo Sobrinho Santos
  • Federal Institute of Northern Minas Gerais, Campus Araçuaí, Minas Gerais, Brazil.
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Hercules Otacílio Santos
  • Hercules Otacílio Santos
  • Federal Institute of Northern Minas Gerais, Campus Araçuaí, Minas Gerais, Brazil.
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Juliana Rezende Sa Miranda Goncalves
  • Juliana Rezende Sa Miranda Goncalves
  • Federal Institute of Northern Minas Gerais, Campus Araçuaí, Minas Gerais, Brazil.
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Anna Christina Almeida
  • Anna Christina Almeida
  • Institute of Agrarian Sciences of UFMG, Campus Montes Claros, Minas Gerais, Brazil.
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Igor Viana Brandi
  • Igor Viana Brandi
  • Institute of Agrarian Sciences of UFMG, Campus Montes Claros, Minas Gerais, Brazil.
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Alex Sander Rodrigues Cangussu
  • Alex Sander Rodrigues Cangussu
  • Federal University of Tocantins, Campus Gurupi, Tocantins, Brazil.
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Janainne Nunes Alves
  • Janainne Nunes Alves
  • Federal Institute of Northern Minas Gerais, Campus Araçuaí, Minas Gerais, Brazil.
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Sabrina Ferreira de Jesus
  • Sabrina Ferreira de Jesus
  • Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
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Ricardo Jardim Neiva
  • Ricardo Jardim Neiva
  • Federal Institute of Northern Minas Gerais, Campus Araçuaí, Minas Gerais, Brazil.
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Kattyanne de Souza Costa
  • Kattyanne de Souza Costa
  • Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
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Andre Luiz Sena Guimaraes4
  • Andre Luiz Sena Guimaraes4
  • Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
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Lucyana Conceiçao Farias
  • Lucyana Conceiçao Farias
  • Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil.
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  •  Received: 11 September 2018
  •  Accepted: 11 October 2018
  •  Published: 15 October 2018

Abstract

This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the  antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells.

Key words: Aroeira honey, Myracrodruon urundeuva, wild honey, Apis mellifera, therapeutic, Jequitinhonha River Valley.