The aim of this study was to determine the effects of high dose atropine and pralidoxime (2-PAM) on different tissues, and to evaluate the drugs’ probable roles in complications during organophosphate (OP) treatment, without using pesticides on healthy tissues. This study was designed as a randomized controlled experimental study consisting of three groups of 10 rats (the control group, the atropine group, and the 2-PAM group). The overall drug dosages were set based on those administered in a previous study to counteract the effects of OP toxicity (0.12 mg/kg parathion-methyl). The drugs were administered in divided doses intraperitoneally (i.p) for 96 h. Sample tissues (that is, heart, lungs, liver, spleen, kidneys, intestines, pancreas, ovaries, and both parotid glands) were evaluated using a light microscope by pathologists blinded to the experiment. The only significant tissue findings were nonspecific findings in the parotid glands of the 2-PAM group and in the secretory glands (parotids, pancreas, and ovaries) of the atropine group identified by light microscope. Atropine contributed to nonspecific cellular changes detected in the secretory glands. Macroscopic evaluation demonstrated that the parotid glands were the only tissues affected by 2-PAM. These nonspecific changes could be the initial state of definite pathological changes and require further study over longer periods with an electron microscope.
Key words: Atropine, pralidoxime, tissue.
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