Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2768

Full Length Research Paper

Tissue effects of high dose atropine and 2-PAM on healthy rats: Examining their role in complications during treatment of organophosphate poisoning

  Betul Gulalp1*, Derya Gumuldulu2, Aysun Uguz2, Zikret Koseoglu3, Gulsah Seydaoglu4, Kenan DaÄŸlıoÄŸlu5 and Ozgur Karcioglu6   .    
  1Emergency Medicine Department, School Of Medicine, Baskent University, Ankara, 06490, TURKEY. 2Pathology Department, School Of Medicine, Cukurova University, Adana, TURKEY. 3Emergency Department, Adana Numune Research and Training Hospital, Adana, TURKEY. 4Biostatistics Department, School Of Medicine, Cukurova University, Adana, TURKEY. 5Experimental Research Center, School Of Medicine, Cukurova University, Adana, TURKEY. 6Emergency Department, School Of Medicine, Acıbadem University, Bakirkoy, Istanbul, Turkey
Email: [email protected], [email protected]

  •  Accepted: 06 July 2012
  •  Published: 28 February 2013



The aim of this study was to determine the effects of high dose atropine and pralidoxime (2-PAM) on different tissues, and to evaluate the drugs’ probable roles in complications during organophosphate (OP) treatment, without using pesticides on healthy tissues. This study was designed as a randomized controlled experimental study consisting of three groups of 10 rats (the control group, the atropine group, and the 2-PAM group). The overall drug dosages were set based on those administered in a previous study to counteract the effects of OP toxicity (0.12 mg/kg parathion-methyl). The drugs were administered in divided doses intraperitoneally (i.p) for 96 h. Sample tissues (that is, heart, lungs, liver, spleen, kidneys, intestines, pancreas, ovaries, and both parotid glands) were evaluated using a light microscope by pathologists blinded to the experiment. The only significant tissue findings were nonspecific findings in the parotid glands of the 2-PAM group and in the secretory glands (parotids, pancreas, and ovaries) of the atropine group identified by light microscope. Atropine contributed to nonspecific cellular changes detected in the secretory glands. Macroscopic evaluation demonstrated that the parotid glands were the only tissues affected by 2-PAM. These nonspecific changes could be the initial state of definite pathological changes and require further study over longer periods with an electron microscope.


Key words: Atropine, pralidoxime, tissue.