Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Antioxidant activity of bamboo-leaf extracts from the species Dendrocalamopsis oldhami

Zhao-Lin Lv1,2, Xi Lin3, Zhi-Hui Miao4, Hong-Xuan Guo3, Jun-An-Hong Wang3, Mei-Ling Lei3, Yue Pan3 and Bo-Lin Zhang2,3*
1Analysis Center, Beijing Forestry University, Beijing, 100083, China. 2Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. 3College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China. 4Institute for Medical Research, Hebei Center for Disease Control and Prevention, Shijiazhuang, Hebei, 050021, China.
Email: [email protected]

  •  Accepted: 24 October 2012
  •  Published: 12 November 2012

Abstract

The antioxidant enzymatic activities of Dendrocalamopsis oldhami bamboo-leaf extracts (BLE) in cholesterol-fed rats and an index of lipid peroxidation were determined. In the BLE groups, compared with the control group, significant decrease was observed in superoxide dismutase (SOD) activity in the livers and kidneys, catalase (CAT) activity in the kidneys, and glutathione peroxidase (GSH-Px) activity in the liver and kidneys. Also, significant decrease was observed in the quantities of thiobarbituric acid reactive substances (TBARS) in the liver and kidneys of the BLE groups. A battery of test methods was used to assess the in vitro antioxidant activities of the extracts. The half maximal inhibitory concentration (IC50) value was found to be 2.36 mg/g by determining the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. The total antioxidant activity was 84 mg vitamin C equivalent in per gram BLE (mg VCE/g BLE), and the reducing power was 74 mg quercetin equivalent in per gram BLE (mg QE/g BLE). It was concluded that sugars, proteins, and flavonoids, which made up 18, 16, and 3.7% of the extract, respectively, could have contributed to the production of the antioxidant benefits.

 

Key words: Bamboo-leaf extractsantioxidant activityflavonoidscatalase (CAT) activityglutathione peroxidase (GSH-Px) activitysuperoxide dismutase (SOD) activity.

Abbreviation

he antioxidant enzymatic activities of Dendrocalamopsis oldhami bamboo-leaf extracts (BLE) in cholesterol-fed rats and an index of lipid peroxidation were determined. In the BLE groups, compared with the control group, significant decrease was observed in superoxide dismutase (SOD) activity in the livers and kidneys, catalase (CAT) activity in the kidneys, and glutathione peroxidase (GSH-Px) activity in the liver and kidneys. Also, significant decrease was observed in the quantities of thiobarbituric acid reactive substances (TBARS) in the liver and kidneys of the BLE groups. A battery of test methods was used to assess the in vitro antioxidant activities of the extracts. The half maximal inhibitory concentration (IC50) value was found to be 2.36 mg/g by determining the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. The total antioxidant activity was 84 mg vitamin C equivalent in per gram BLE (mg VCE/g BLE), and the reducing power was 74 mg quercetin equivalent in per gram BLE (mg QE/g BLE). It was concluded that sugars, proteins, and flavonoids, which made up 18, 16, and 3.7% of the extract, respectively, could have contributed to the production of the antioxidant benefits.

 

Key words: Bamboo-leaf extractsantioxidant activityflavonoidscatalase (CAT) activityglutathione peroxidase (GSH-Px) activitysuperoxide dismutase (SOD) activity.