Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2768

Full Length Research Paper

Molecular cloning and analysis of a novel HMW-GS gene Glu1-St1.5 from Elymus sibiricus in Qinghai-Tibetan Plateau

LIU Jin1,2,3#, ZHANG Bo1#, LIU Bao-long1, LI Hong-qin1,2, WANG Xin1,2, LIU Qi1,2, WEI Le1,2 LIU Deng-cai1,4 and ZHANG Huai-gang1*   # These authors contributed equally to this paper.
  1Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining 810008, China.     2Graduate University, Chinese Academy of Sciences, Beijing 100049, China.   3Yunnan Institute of Tropical Crops, Xishuangbanna 666100, China. 4Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China.
Email: [email protected]

  •  Accepted: 30 May 2012
  •  Published: 05 July 2012



In the present study, a HMW-GS allele Glu1-St1.5 was isolated and characterized from Elymus sibiricus. Nucleotide sequence analysis indicated that the St1.5 shared the highest similarity with St1.3 from Elymus canadensis, but the “TA” insertion mutation at 15 to 16 bp in signal peptide sequence made it an inactive gene, because three premature stop codons were generated by “TA” insertion in signal peptide region, N-terminal region and C-terminal region, respectively. To identify the type of St1.5, the inserted “TA” was eliminated factitiously and the deduced amino acid sequence was analyzed, the result showed that St1.5 was a y-type HMW-GS pseudo gene. Phylogenetic tree demonstrated that St1.5 had the closest relationship to the St1 allele ofPseudoroegneria stipifolia, which indicated that St1.5 was a novel gene of St Genome. These results provide insights into the evolutionary biology of Glu1-St1.5 and other HMW-GS genes.


Key words: Glu1-1St1.5, HMW-GS, Elymus sibiricus.