Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2748

Full Length Research Paper

Characterization of WPI-NaCas composite films modified by transglutaminase

Lei Qiao
  • Lei Qiao
  • School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, China.
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Pan Jiazhen
  • Pan Jiazhen
  • School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, China.
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Huang Zhiying
  • Huang Zhiying
  • College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
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Bao Jianqiang
  • Bao Jianqiang
  • College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
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Xun Qiannan
  • Xun Qiannan
  • College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
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  •  Received: 03 January 2013
  •  Accepted: 02 March 2013
  •  Published: 15 May 2014

Abstract

Synergistic effects of additive amount, reaction time and temperature of transglutaminase on properties of WPI-NaCas composite protein films were discussed by orthogonal experiments. Results indicated that transglutaminase (TGase) modification decreased D50 and D90 of film-forming solution particles, resulting in decrease in water solubility and increase in both mechanical and water barrier properties of films under most conditions. Compared with control sample, scanning electron microscope analysis indicated that cross-section of enzyme modification sample was denser and more impermeable. However, there was no significant difference in both thermogravimetric analysis and solution apparent viscosity changes for all samples. Orthogonal experiment results also showed that TGase reaction time was the most effective factor on tensile strength  (TS)  and water vapor permeability (WVP) properties of film, and the secondary, tertiary factors were reaction temperature, TGase additive amount respectively. Films modified by 0.1 mg/ml TGase at 50°C for 30 min exhibited the optimum mechanical and water barrier performance.

 

Key words: Transglutaminase (TGase), mechanical properties, water vapor permeability (WVP), scanning electron microscopy analysis.