Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Efficient DNA isolation from Emblica officinalis for effective PCR

Vishal Bharmauria*, Vivek Verma, Navjyoti Narang and Shalini Sharma
Shoolini Institute of Life Sciences and Business Management, Himachal Pradesh University, Solan, 173212, India.
Email: [email protected]

  •  Accepted: 28 January 2009
  •  Published: 31 January 2010

Abstract

Emblica officinalis is a medicinal plant and distributed throughout the lower ranges of Himachal Pradesh. Its ph is low and has high amount of polysaccharides, polyphenols several pigments and other secondary metabolites. A slight modification of Warude et al. (2003) protocol for DNA isolation yielded good quality high molecular weight DNA free of contaminants from eight plants taken for analogy using RAPD as a potential marker. 120 mgPVP/g of tissue was used and the isolated DNA pellets were washed twice with 70% ethanol.

 

Key words: Himachal Pradesh, medicinal plant, Emblica officinalis,polysaccharides, PCR, RAPD, PVP.