Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Chronic use of phenytoin reversibly suppresses fertility in male Sprague-Dawley rats

  P. Falokun Olutunde1, O. Salawu Emmanuel1,2*, S. Ajao Moyosore3, A. Adeeyo Olusola4, O. Oyewo Olutoyin4, A. Ashamu Ebenezer4, Oyerinde Abiodun1 and J. Onaolapo Olakunle5        
  1Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso,Oyo State, Nigeria 2Department of Human Physiology, Faculty of Medicine, Ahmadu Bello University, Zaria, Nigeria 3Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, Ilorin, Nigeria 4Department of Anatomy Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria 5Department of Pharmacology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Email: [email protected]

  •  Accepted: 01 April 2010
  •  Published: 31 May 2010



This study investigates the effect of chronic use of Phenytoin on male reproductive functions. Sixty male Sprague-Dawley rats were used. They were divided into five groups of 12 rats each (A, B, C, D and E). Group A served as the control and was given 0.1 ml of normal saline. Group B and C received 50 mg/Kg of Phenytoin. Group D and E were given 100 mg/Kg of Phenytoin. Administration of the drug was orally and twice daily for four weeks. After the first two weeks of administration, twenty-four mature untreated female rats with proven fertility were cohabited with animals in Group B and with those in Group D, in ratio 1:1. The administration continued for another two weeks during the co-habilitation. At the end of the fourth weeks, the animals in Group B and D were sacrificed. While the animals in Group C and E were allowed to recover for another four weeks, during which they were also tested for fertility, and eventually sacrificed. The results obtained were analyzed using t-test. The results show that Phenytoin significantly (p-value < 0.05) reduced fertility in male rats. Its effects were, however, reversible upon withdrawal.


Key words: Phenytoin, chronic use, reversibly, male fertility, testosterone, Sprague-Dawley rats.