Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

A novel rat model of 1/4 partial nephrectomy and its application in ischemia-reperfusion research in residual kidney

Qianglin Duan1,2, Guangchun Wang2 and Junhua Zheng2*
1Department of Scientific Research, Tongji Hospital, Tongji University, No.389 Xincun Road, Shanghai 200065, China. 2Department of Urology, Tenth People’s Hospital, Tongji University, No.301 Yanchang Road, Shanghai 200072, China.
Email: [email protected]

  •  Accepted: 11 June 2010
  •  Published: 04 July 2010

Abstract

This study established a new rat model of 1/4 partial nephrectomy for research on ischemia-reperfusion injury in residual kidney. Thirty healthy Sprague-Dawley rats were intraperitoneally anesthetized by pentobarbital sodium. A lumbar oblique incision was made, and then the right renal pedicle was isolated and clamped by a smooth microclip. The kidney size was measured and the upper pole of  the right kidney (about 0.5 × 1.0 cm) was resected by a scorching knife, then SURGICEL* FIBRILLAR Absorbable Hemostats (oxidized regenerated cellulose) was used for rapid haemostasis. Mean kidney size was 2.0 × 1.1 cm, and mean kidney tissue removed actually was 0.6 × 1.0 cm. Mean operation time was 3.2 ± 0.5 min  (2.6 - 5.2 min), and mean haemostasis time after resection was 1.2 min (0.8 - 1.5 min). Three minutes after partial nephrectomy, the smooth microclip was removed for reperfusion, without rebleeding in all cases. No rats died during the operation. A new 1/4 partial nephrectomy rat model was successfully established in our study, which can be used for research on ischemia-reperfusion injury in residual kidney. Rapid haemostasis could be achieved by SURGICEL* FIBRILLAR Absorbable Hemostats, which is suitable for application in surgical experiments in rats. 

 

Key words: Partial nephrectomy, ischemia-reperfusion injury, haemostasis, rat model.