Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Further study on molecular biological effects of CD4 D1 inhibitor J2 on allografts following corneal transplantation in mice

Da-Jiang Wang1, Han Zhang1, Guo-Jiang Chen2, Li-Qiang Wang2, Ruo-Nan Xu2, He Xiao2, Ren-Xi Wang2, Ming Yu2, Yan Li2, Song Li3 and Yi-Fei Huang1*
1Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, China. 2Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing 100850, China. 3Department of Molecular Drug Design, Institute of Pharmacology and Toxicology Sciences, Beijing100850, China.
Email: [email protected]

  •  Accepted: 02 December 2009
  •  Published: 31 January 2010

Abstract

Our previous experiments have revealed that a non-peptide organic ligand of CD4 D1-J2 as a potential inhibitor of CD4 D1 can inhibit corneal allograft injection and prolong the corneal allograft survival. The present study aimed to further investigate molecular biological effects of J2 treatment on allografts following corneal transplantation in mice. Allogeneic orthotopic penetrating keratoplasty was performed using C57BL/6 mice as donors and BALB/c mice as recipients. Allograft recipient mice were randomly divided into J2-treated group, CsA-treated group (positive control) and DMSO treatment group (negative control). After treatment for consecutive 12 days, T cell phenotype analysis, CD4, CD8 immunohistochemical staining, T-cell proliferation and mRNA expression analysis of cytokines were performed. The subsequent molecular biological assays revealed that the immunosuppressive activity of J2 was associated with its inhibitory effects on the CD4+ T cells and cells-mediated responses. J2 is as effective as CsA in prevention of allograft rejection following corneal transplantation. 

 

Key words: CD4+ T cells, immunosuppressive agent, CD4 D1 inhibitor, CsA, corneal transplantation.