A simple method is used for the determination of patulin in apple juice. The samples were extracted with ethyl acetate and the extract was cleaned up by using sodium carbonate solution. Patulin was then determined reverse phase high performance liquid chromatography (HPLC) using a 25 cm Zobrax XDB C18column and a variable wavelength detector set at 276 nm. Water-Acetonitrile (4:96) was used as the mobile phase at a flow rate of 1 ml/min. The occurrence of patulin as a toxic mycotoxin contamination was investigated in 120 samples of apple Juice commercially available in Saudi Arabia by using an HPLC method with a concentrations ranges from 0.057 to 0.104 μg/ml. The primary aim of this investigation was to determine the amount of patulin content in apple juice and pay attention that it may cause health problems to the consumer.
Key words: Mycotoxins, patulin, apple juice, HPLC.
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