Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Transgenic Pinus armandii plants containing BT obtained via electroporation of seed-derived embryos

X. Z. Liu1, H. L. Li2, R. H. Lou1, Y. J. Zhang1 and H. Y. Zhang1*
  1Key Laboratory of Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, Yunnan Province-650224, P. R. China. 2Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Dangzhao, Hainan, People’s Republic of China, 571737.
Email: [email protected]

  •  Accepted: 13 October 2010
  •  Published: 18 November 2010

Abstract

 

A genetic transformation system for Pinus armandii was presented using electroporation for gene delivery and mature embryo as the gene target. Plasmid DNA (pBSbtCryIII(A)), which contained a selectable npt// gene for resistance to the kanamycin and a synthetic chimeric gene SbtCryIII(A) encoding the insecticidal protein btCryIII(A), was delivered into mature P. armandii embryos via electroporation. Transformed plants were identified by their ability to grow on a selective medium containing 50 mg/L of kanamycin. Plant resistance to the application of kanamycin, PCR and southern hybridization indicated thatSbtCryIII(A) genes had integrated into the P. armandii genome. Results of Southern blot hybridization indicated that some PCR positive plants might be inlaid.

 

Key words: Electrotransformation, insect-resistant gene, mature embryo, DNA hybridisation, Pinus armandii.