Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2763

Article in Press

Minimum Inhibitory Concentration and Remediation Potential of Pseudomonas aeruginosa Isolated from Polluted soil

Olusola Adelowotan, Emmanuel Ayodele Oluyemi and Anthonia O. Oluduro

  •  Received: 08 December 2022
  •  Accepted: 14 April 2023
This study evaluated the heavy metal resistance and removal efficiency of metal ion from aqueous and soil solution using Pseudomonas aeruginosa isolated from polluted soil. Minimum Inhibitory Concentration was determined by streaking the isolate on nutrient agar incorporated with selected metal salt and gradual increase in the concentration of each solution by 20 ppm. Percentage removal of cation from aqueous solution was evaluated by adding standardized 2 ml of inoculum to 30 ppm of each metal solution containing nutrient broth. Equivalent milliliter of inoculum was added to a solution of 8 ml of nutrient broth containing 1g of polluted soil in a test-tube. Initial concentration of metals in both aqueous and soil solution before the addition and after removal of inoculum was determined by Atomic Absorption Spectrometry. Isolate biomass was subjected to Fourier Transform Infrared spectroscopy for determination of functional groups. MIC results show high degree of tolerance to selected heavy metals concentrations in decreasing order of Ni2+ > Pb2+> Cu2+ > Cr2 > Cd2+. Isolate was effective in the reduction of nickel, lead, copper, chromium and cadmium concentrations in aqueous solution with the following percentage removal efficiency of 46.81%, 63.32%, 69.67%, 32.39% and 60.15%. Effective reduction of selected metals concentrations were also observed in polluted soil samples collected from four different sites with maximum percentage reduction of Cd to non-detected level, 57.86% reduction of Cu , 54.86% reduction of Cr, 53.11% reduction of Ni and 50.04% of Pb

Keywords: Aqueous solution, Heavy metal, Isolate, Minimum Inhibitory Concentration