Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2754

Full Length Research Paper

Development of a method to detect infectious rotavirus and astrovirus by reverse transcriptase polymerase chain reaction (RT-PCR) with propidium monoazide (PMA)

Wei Yumei
  • Wei Yumei
  • College of Biological Sciences and Technology, P. O. Box 162, Beijing Forestry University, Beijing 100083, China
  • Google Scholar
Liu Tingting
  • Liu Tingting
  • College of Biological Sciences and Technology, P. O. Box 162, Beijing Forestry University, Beijing 100083, China
  • Google Scholar
Wang Jingqi
  • Wang Jingqi
  • College of Biological Sciences and Technology, P. O. Box 162, Beijing Forestry University, Beijing 100083, China
  • Google Scholar
He Xiaoqing
  • He Xiaoqing
  • College of Biological Sciences and Technology, P. O. Box 162, Beijing Forestry University, Beijing 100083, China
  • Google Scholar
Wang Zijian
  • Wang Zijian
  • State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China.
  • Google Scholar


  •  Received: 03 January 2013
  •  Accepted: 02 March 2013
  •  Published: 15 May 2014

References

Bae S, Wuertz S (2009). Rapid decay of host-specific fecal Bacteroidales cells in seawater as measured by quantitative PCR with propidium monoazide. Water Res. 43(19):4850-4859.
Crossref
 
Belliot G, Laveran H, Monroe S (1997). Detection and genetic differentiation of human astrovirues:phylogenetic grouping varies by coding region. Arch. Virol. 142:1323-1334.
Crossref
 
Dubios E, Le Guyader F, Haugarreau LK, Cormier H, Pommepuy M (1997). Molecular Epidemiological Survey of Rotaviruses in Sewage by Reverse Transcriptase Seminested PCR and Restriction Fragment Length Polymorphism Assay. Appl. Environ. Microbiol. 63(5):1794-1800.
 
Haramoto E, Katayama H, Oguma K, Ohgaki S (2005). Application of cation-coated filter method to detection of noroviruses, enteroviruses, adenoviruses, and torque teno viruses in the Tamagawa River in Japan. Appl. Environ. Microbiol. 71(5):2403-2411.
Crossref
 
Maunula L, Klemola P, Kauppinen A, Söderberg K, Nguyen T, Pitkänen T, Kaijalainen S, Simonen ML, Miettinen IT, Lappalainen M, Laine J, Vuento R, Kuusi M, Roivainen M (2008). Enteric Viruses in a Large Waterborne Outbreak of Acute Gastroenteritis in Finland, Food Environ. Virol. 1(1):31-36.
Crossref
 
Nocker A, Camper AK (2009). Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol. Lett. 291(2):137-142.
Crossref
 
Nocker A, Cheung CY, Camper AK (2006). Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs dead bacteria by selective removal of DNA from dead cells. J. Microbiol. Methods. 67(2):310-320.
Crossref
 
Pan Y, Breidt F Jr (2007). Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Appl. Environ. Microbiol. 73(24):8028-8031.
Crossref
 
Parshionikar S, Laseke I, Fout GS (20101). Use of propidium monoazide in reverse transcriptase PCR to distinguish between infectious and noninfectious enteric viruses in water samples. Appl. Environ. Microbiol. 76(13):4318-4326.
Crossref
 
Prüss A, Kay, D, Fewtrell L, Bartram J (2002). Estimating the burden of disease from water, sanitation, and hygiene at a global level. Environ. Health Perspectives. 110(5):537-542.
Crossref