In silico detection and correlative analysis of antibiotic resistance plasmid-incompatibility (Inc/ rep) groups from different environments
October 2019
Plasmids are extra-chromosomal mobile DNA elements found mostly in bacteria, and often serve as vectors for accumulation and transfer of antibiotic resistance genes (ARGs) within bacterial populations in different environments. Plasmid mediated transfer of ARGs has accounted for the spread of antibiotic resistance within clinically relevant pathogenic bacteria; this has escalated into a global health problem. Plasmids...
Relationship and prediction of body weight from morphometric traits of indigenous highland sheep In Tigray, Northern Ethiopia
March 2017
The study was conducted in Atsbiwonberta district to determine the relationship between live weight and linear measurements and predict live weight from morphometric traits. Three potential peasant associations namely, Habes, GolgolNeale and Gebrekidan were selected. Randomly selected 257 sheep (121 males and 136 females) were grouped into four age groups (0 PPI, 1 PPI, 2 PPI, and 3 PPI). Live body weight was highly and...
Effect of ascorbic acid on blackening and sprouting of Musa spp shoot tips
October 2012
The effect of ascorbic acid (0, 5, 10, 20, 30, 40 and 150 mgl-1) on blackening and sprouting of Musa spp genotypes was investigated using shoot tip culture. Ascorbic acid did not minimize blackening, but rather markedly reduced the sprouting of cultured shoot tip explants. Ascorbic acid, genotype and genotype x ascorbic acid interactions were significant for shoot tip blackening and...
Studies on the optimization of bioelectricity production from industrial and domestic waste using immobilization of microbial cells
October 2012
A unique type of fuel cell with a potential in long-term is the bio fuel. The data obtained from the studies undertaken reflect glucose yielding the peak voltage. However, sucrose is cheap, similar to glucose in the electricity production pattern. Using glucose in anaerobic culture conditions, the microbial fuel cell produced a peak voltage of 1.35 by organisms Saccharomyces cerevisiae but different current...
Computational studies on Ribavirin binding to RNA-dependent RNA polymerase, inducing Crimean-Congo hemorrhagic fever
April 2012
The Crimean-Congo hemorrhagic fever is a viral disease, widespread amongst domestic as well as wild animals, and probably may affects humans too. This virus belonging to Bunyaviridae family contains viral RNA-dependent RNA polymerase catalyzing the biosynthesis of the RNA strands. As a consequence of inhibition of a target enzyme protein, namely, RNA-dependent RNA polymerase there is a...
Identification of Zinnia leaf curl virus infecting Zinnia elegans in India
April 2012
In a survey during 2007 to 2009 at Gorakhpur and nearby locations of North Eastern Uttar Pradesh, India leaf curling, foliar deformation and distortion symptoms were observed on Zinnia elegans plants. The associated White fly population indicated the possible presence of begomovirus in the field. Therefore, Polymerase chain reaction (PCR) was performed with the begomovirus specific primers (TLCV-CP). Total...
Investigation of the role of calcium in prolonging the shelf life of fluted pumpkin (Telefairia occidentalis Hook F.)
September 2011
A 16-week study using 2 certified land races of Telfairia occidentalis Hook F plants, to ascertain the effectiveness of calcium in prolonging the shelf life of the leaves and identify the pathogens that cause leaf rot of the plant was carried out. The two varieties were subjected to 5 levels of calcium carbonate treatments (0.25, 0.50, 0.75, and 1 g and control (0 g) in 20 kg...
Protein engineering of yellow fluorescent protein insertion in outer membrane protein OmpF from Salmonella typhi
September 2011
Each monomer of the trimeric outer membrane porin OmpF of Salmonella typhi folds as a 16-stranded β-barrel with eight short turns at the periplasmic side and eight loops on the cell surface side. Yellow fluorescent protein (YFP) has been engineered into Loop 7 of OmpF. HindIII and EcoRV restriction sites were introduced in the region corresponding to Loop 7 of ompF gene....
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