January 2016

A COMPARISON OF THE EFFECT OF COMMERCIAL pH BUFFERS ON EPITOPE RETRIEVAL OF ‘HER-2/neu’ PROTEIN

Aim: The significance of immunohistochemistry standardization became important with the disclosure of alarmingly high records of inconsistent results in the assessments of HER-2/neu expression status in women with breast cancers. Formalin-Fixed-Paraffin-Embedded tissue usually presents with conformational macromolecular changes, masking the epitope with sub-optimal staining of HER- 2/neu protein. The pH of epitope...

Author(s): Enemari JO, Avwioro OG, Osadolor HB, Idoko J, Yaro JD, and Odigie BE