September 2018

Effect of formalin fixation on DNA purity and quantity, nucleic acid, and amplicon size in cervical human papilloma virus detection

Tissue fixation with 10% formalin and molten paraffin wax embedding (FFPE) is routinely used protocol for tissue preservation in histopathology laboratory. We therefore aimed at comparing the differences in DNA quantity, DNA purity, nucleic acid and its effect on primers (PCR) amplicon (bp) sizes between fresh cervical tissues and formalin fixed paraffin embedded (FFPE). The differences in DNA purity, quantity and...

Author(s): Oboma Y. I. and Ngokere A. A.