April 2012

cDNA, genomic sequence cloning and over-expression of ribosomal protein P2 gene (RPLP2) from the Giant Panda (Ailuropoda melanoleuca)

RPLP2 is a component of the 60S large ribosomal subunit encoded by RPLP2 gene and directly participate in protein synthesis, which is located in the cytoplasm. The cDNA and genomic sequence ofRPLP2 were cloned successfully from the Giant Panda using RT-PCR and Touchdown-PCR technology, respectively. The results showed that the cDNA fragment cloned is 394 bp in size, containing an open reading frame of 348...

Author(s): Sinan Zhang, Yiling Hou, Wanru Hou, Xiang Ding and Chunlian Wu

April 2012

Antibiotic resistance profile of Escherichia coli isolated from apparently healthy domestic livestock in South-East Nigeria

This study was conducted to determine the antibiotic resistance profile of Escherichia coli isolated from apparently healthy domestic livestock viz: cattle, goats, poultry and swine from three states in South East of Nigeria. E. coli was isolated using Eosin Methylene Blue agar (EMB) and identified by conventional microbiological technique. The isolates were tested against 14 antibiotics using...

Author(s): Chijioke A. Nsofor and Christian U. Iroegbu

April 2012

Effect of Fufang Jiangzhi No. 3 on cholesterol-bile acid metabolism in New Zealand white rabbit fed with cholesterol-rich diet

The effect of Fufang Jiangzhi No. 3 on cholesterol-bile acid metabolism in New Zealand white rabbit fed with cholesterol-rich diet was studied. 24 male New Zealand white rabbits were randomly assigned into control group (Group A), hypercholesterolemia model group (Group B), and Fufang Jiangzhi No. 3 treatment group (Group C). Groups B and C were fed with cholesterol-rich diet (containing 1% cholesterol) 120 g/day during...

Author(s): Qiqi Mao, Dongni Qiu, Xiaodong Fu, Yi Liu, Wenjian Wang and Xu Sun