August 2006
Fatty acids isolated from Milletia versicolor Baker (Fabaceae)
Milletia versicolor Baker is a plant used in the Congolese traditional medicine for the treatment of intestine parasitosis. A chemical study carried out from its methanolic extract has concluded with the isolation using gas chromatography/mass spectrometry (GC/MS) of four organic acids; tetradecanoic acid, pentadecanoic acid, n-hexadecanoic acid and octadecanoic acid. Key words: Milletia...
August 2006
Application of simple sequence repeats (SSRs) markers to study the resistance of locally adapted maize hybrids to damaging maize streak virus disease
Simple sequence repeat markers were used to study S6 recombinant inbred lines originating from a single cross between two inbred lines OSU 23i and EM12-210, for resistance to maize streak virus disease. A total of 115 recombinant inbred lines belonging to three families previously selected using conventional methods were screened using 52 SSR markers contained between Bin 1.04 and 1.05 of maize chromosome 1. Of...
August 2006
Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deepwater rice (Oryza sativa L.) cultivars of Bangladesh
Experiments were carried out to induce embryogenic callus and plant regeneration from five different deepwater rice cultivars. Using mature embryos as explant, all the cultivars demonstrated high callus induction and plant regeneration frequencies. When coleoptile and root segments used as explants, they had low frequencies of embryogenesis. Depending on different genotypes, the best plant regeneration was obtained on...
August 2006
Genetic diversity in cocoa germplasm of southern Cameroon revealed by simple sequences repeat (SSRS) markers
The range of polymorphism of about 194 cocoa accessions collected in farms in Southern Cameroon during field surveys and 71 Trinitario and Upper Amazon clones available in genebanks on-station was assessed using 13 SSR markers. The gene diversity, genetic differentiation and genetic similarities were analysed for the different populations. In total, 282 alleles were detected within all the populations studied. The farm...
August 2006
Occurrence of diploid and polyploid microspores in Sorghum bicolor (Poaceae) is the result of cytomixis
Colchicine is used in this investigation to increase chromosome doubling in Sorghum bicolor Line IS4546. Of a total of 500 plants which were treated, eight of them showed the occurrence of cytomixsis at meiotic stages. No polyploids were found in the treated plants. The results obtained from meiotic studies on control plants showed the 10 bivalents in majority of pollen mother cells at diakinesis and first metaphase. In...
August 2006
Polymorphisms in the GST (M1 andT1) gene and their possible association with susceptibility to childhood acute lymphocytic leukemia in Indian population
Glutathione S-transferases (GSTs) are enzymes involved in the detoxification of several environmental mutagens, carcinogens and anticancer drugs. GST polymorphisms resulting in decreased enzymatic activity have been associated with several types of solid tumors. We determined the frequencies of thedeletion of two GST subfamilies genes, M1 and T1, in patients with acutelymphocytic...
August 2006
Water-condition effects on rhizobia competition for cowpea nodule occupancy
Two indigenous bradyrhizobia strains displaying different natural behaviours towards water regime (strain ORS 3257, nodulating more frequently in favourable-water conditions and strain ORS 3260, in limited-water conditions) were studied for their competitivity for nodulation of cowpea (Mouride cultivar) under favourable and limited water conditions in non-sterile soil. The nodule occupancy was studied by...
August 2006
Optimization of sub-merged culture conditions for biomass production in Pleurotus florida (mont.) Singer, a Nigerian edible fungus
Submerged culture conditions such as physical factors, chemical compounds and inoculum sizes were optimized for biomass production in Pleurotus florida, a Nigerian edible mushroom. This fungus produced the greatest biomass at pH of 6.5 and 30°C. Among the monosaccharides, glucose stimulated the best biomass production (186.7 mg/30 cm3) followed in order by fructose mannose, and sorbose (P ≤...
August 2006
In vitro flowering in embryogenic cultures of Kinnow mandarin (Citrus nobilis Lour ´ C. deliciosa Tenora)
Embryogenic cultures of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora) were raised from unfertilized ovules dissected from unopened flower buds of this plant inoculated on MS medium supplemented with 2 mg/L kinetin (KN). In vitro flowering was induced in these cultures by using different concentrations of KN and sucrose as well as subjecting these cultures to different photoperiods. Maximum...
August 2006
Lead (II) and nickel (II) adsorption kinetics from aqueous metal solutions using chemically modified and unmodified agricultural adsorbents
This paper discusses the kinetics of lead (II) and Nickel (II) ions adsorption from aqueous solutions using chemically modified and unmodified agricultural adsorbents at 28°C, pH 6.2 and 0.01M NaCl ionic strength. The removal of the two metals were found to increase with increase in chemical modification, the sequence being 1.0MOPF>0.5MOPF>UOPF. In addition Nickel (II) had a higher percentage...
August 2006
Industrial wastewater treatment using natural material as adsorbent
Attempts were made to compare the adsorption efficiency of coconut shell-based granular activated carbon with the adsorption efficiency of commercial carbon, Calgon carbon F-300, with respect to adsorption of organic matter from a beverage industrial wastewater. Freundlich adsorption isotherm was used to analyze the adsorption efficiencies of the two activated carbons. These studies indicate that acid-activated coconut...
August 2006
Growth, proline and ion accumulation in sugarcane callus cultures under drought-induced osmotic stress and its subsequent relief
Calli obtained from two sugarcane cultivars (R570 and CP59-73) were exposed to different osmotic stress intensities followed by a period of stress relief. Relative rate growth, callus water content and changes in organic and inorganic solutes were determined at the end of stress and relief periods. After the stress period, calli derived from both cultivars showed a decrease in RGR, but at lesser extent in R570 than...
August 2006
Effect of supplementation of African breadfruit (Treculia africana) hulls with organic wastes on growth characteristics of Saccharomyces cerevisiae
African breadfruit (Treculia africana) hulls were supplemented at different levels with other organic food processing wastes (orange, plantain, cassava and soybean). Optimum supplementation of 40:60 (breadfruit hulls to each waste) was obtained. Proximate and mineral composition of the unsupplemented and the supplemented waste were determined. A synthetic medium with chemical nutrients was prepared. The growth...
August 2006
Effect of fermentation and drying temperature on the characteristics of goat meat (Black Bengal variety) dry sausage
A new variety of fermented goat meat sausage was prepared from Black-Bengal goat. Lactic acid bacteria (LAB) Lactobacillus casei (NCIM 2586), Lactobacillus plantarum (NCIM 2083) and Pediococcus pentosaceus (NCIM 2245) were used as starter culture. Effect of temperature during fermentation and drying steps of sausage preparation was studied with respect to change in pH, lactic acid...
June 2006
Effect of gamma irradiation on moisture sorption isotherms of cowpeas
The effect of gamma irradiation on moisture sorption isotherms of cowpeas was investigated The non-irradiated and irradiated cowpeas exhibited the typical three stage sigmoidal curve found in most foods. There was also a concomitant increase in the equilibrium moisture content (EMC) as relative humidity increased for non- and irradiated cowpeas. Drum cowpea (15 Gy) had the highest rate of water absorption. Generally,...
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