December 2013

Genotyping of Toxoplasma gondii samples from Dakar

Toxoplasmosis is an anthropozoonosis of medical and veterinary importance, caused by the protozoan Toxoplasma gondii. Oocysts shed by felids play a key role in parasite transmission as they contaminate meat-producing animals, vegetables and water consumed later by humans. In this work, we aimed to identify T. gondii isolated in Dakar (Senegal). The modified multiplex PCR technique based on length polymorphism of 15...

Author(s): Amadou Ndiaye, Pape Mbacké Sembene, Ngor Faye, Daouda Ndiaye, Daniel Ajzenberg, Niama Diop Sall, and Ben Sikina Toguebaye

December 2013

Detection of antibiotic resistance genes of Escherichia coli from domestic livestock in south east Nigeria with DNA microarray

DNA microarray was developed for detection of up to 90 antibiotic resistance genes in Escherichia coli by hybridization. Each antibiotic resistance gene was represented by two specific oligonucleotides chosen from consensus sequences of gene families. A total of 203 oligonucleotides (50-100 base) were spotted onto the microarray. The sequence identity of each gene was compared with GenBank sequen-ces, biotin was used as...

Author(s): Chijioke A. Nsofor, Christian U. Iroegbu Douglas R. Call and Margaret A. Davies