July 2013

Comparative study on solubilization of tri-calcium phosphate (TCP) by phosphate solubilizing fungi (PSF) isolated from Nsukka pepper plant rhizosphere and root free soil

Phosphate solubilizing fungi (PSF) possessing the ability to solubilize insoluble phosphate was isolated from the rhizospheric soil of Nsukka pepper plant. Twelve (12) fungal strains were isolated from both root-free soil (non-rhizosphere) and rhizosphere of Nsukka pepper plant. Of the 12 fungi isolated, 6 strains were from Nsukka pepper plant rhizosphere whereas the other six were from root- free soil. PF...

Author(s): Onyia, Chiadikobi Ejikeme and Anyanwu, Chukwudi Uzoma

July 2013

Aspergillus niger-specific ribonucleic acid extraction method

Extraction of high quality RNA from Aspergillus niger mycelia, a tissue very rich in ribonucleases was achieved using a protocol involving the use of TRIZOL reagent and the ribonuclease inhibitor (RNaseOUT). RNA was successfully isolated using this protocol that involved addition of RNaseOUT at the initial homogenizing step and the final dilution/ solubilisation steps of the RNA extraction. A sharply...

Author(s): Abdullahi Balarabe Sallau, Fiona Henriquez, Andrew Jonathan Nok, Sani Ibrahim, Caroline Sommerville and Craig Roberts