International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010

IJBMBR Articles in press

Article in Press

Forward genetics in smut fungi: Creation of mating defective mutants of Sporisorium scitamineum for gene discovery

Sexual phases among pathogenic fungi are major determinants of morphological transitions linked to virulence. The sugarcane smut fungi, Sporisorium scitamineum, are an obligate bitrophic pathogen, whose molecular basis of sexual mating is poorly understood. Here we report the creation of a library of mating-defective mutants of S. scitamineum using Agrobacterium mediated random insertional mutagenesis in the quest to...

Author(s):Tinashe Josiah Runyanga, Yubin Chen and Fei Sun

Article in Press

Effect of 2, 4-dichlorophenoxy acetic acid and naphthelene acetic acid concentrations on callogenesis for in vitro regeneration in selected sugarcane varieties

This study investigated the effect of 2,4-dichlorophenoxyacetic acid (2, 4-D) and naphthalene-1-acetic acid (NAA) concentrations on callus induction and shoot regeneration in three sugarcane varieties: CO945, CO421, and N14. Young leaf spindle explants were cultured on Murashige and Skoog (MS) basal medium supplemented with 2, 4-D (0.0, 2.0, 2.5, 3.0, 3.5 and 4.0 mg L-1) and NAA (0.0 and 1.0 mg L-1). Experiments were laid...

Author(s):RICHARD WEKESA, Justus Onguso, Bernard Nyende, Leonard Wamocho

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