African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12480

Table of Content: 10 January 2011; 10(2)

January 2010

New chromosome numbers in the genus Trigonella L. (Fabaceae)from Turkey

  Somatic chromosome numbers of 45 Trigonella L. (Fabaceae), collected from different localities in Turkey was examined. Chromosome numbers were determined as 2n = 14, 16, 30 and 46. B chromosome was also observed in somatic cells of some taxa (Trigonella arcuata C.A. Meyer and Trigonellaprocumbens (Besser) Reichb.). In addition, one or two...

Author(s): E. Martin, H. Akan, M. Ekici and Z. Aytaç

January 2010

Molecular analysis and expression of phenylalanine ammonia-lyase from poinsettia (Euphorbia pulcherrima willd.)

  Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) is a key regulatory enzyme that link primary and secondary metabolism in plants by catalyzing the conversion of l-phenylalanine to cinnamic acid. In this study, the cDNA and genomic DNA of PAL(named EpPAL) in poinsettia (Euphorbia pulcherrima willd.) were isolated and submitted in GenBank with accession number FJ594466 and FJ943503,...

Author(s):   Jun Tao, Chunyan Cao, Daqiu Zhao, Chunhua Zhou and Guohua Liang  

January 2010

Transfer of stripe rust resistance from Aegilops variabilis to bread wheat

  In terms of area, the bread wheat producing regions of China comprise the largest area in the world that is constantly threatened by stripe rust epidemics. Consequently, it is important to exploit new adult-plant resistance genes in breeding. This study reports the transfer of stripe rust resistance from Aegilops variabilis to bread wheat resulting in resistant line, TKL2(R). Genetic...

Author(s): Dengcai Liu,, Zhiguo Xiang, Lianquan Zhang, Youliang Zheng, Wuyun Yang, Guoyue Chen , Chunjie Wan  and Huaigang Zhang

January 2010

Analysis of genetic diversity in chickpea (Cicer arietinum L.) cultivars using random amplified polymorphic DNA (RAPD) markers

  Genetic diversity of seven chickpea (Cicer arietinum L.) cultivars of Pakistani origin was analyzed by using random amplified polymorphic DNA (RAPD) markers, an extremely effective method to determine the variations among the chickpea cultivars. Polymerase chain reaction (PCR) conditions were optimized for RAPD and the conditions which gave the optimized results were selected for further...

Author(s): Zahid Mahmood, Mohammad Athar, Muhammad Aurangzeb Khan, Muhammad Ali, Shahzadi Saima and Altaf A. Dasti

January 2010

Evaluating potential of borage (Borago officinalis L.) in bioremediation of saline soil

Bioremediation is an efficient, economical and environmentally acceptable strategy used for coping with the salinization of agricultural soils. In this study, borage has been proposed as possible candidate for bioremediation of Na+ and Cl−. In this order, the borage (Borago officinalis L.) seeds were sown under four levels of salinity (non-saline as control, 5, 10 and 15 dSm-1). Bioremediation and...

Author(s): Hassanali Naghdi Badi and Ali Sorooshzadeh

January 2010

Analysis of G × E interaction using the additive main effects and multiplicative interaction (AMMI) in potato cultivars

  This experiment were conducted on the 3 cultivars of potato (Agria, Satina and Caesar) and 4 irrigation regimes (after 30 mm evaporation from class A evaporation pan, after 30 mm evaporation from class A evaporation pan with spraying by potassium humate, after 60 mm evaporation with spraying by potassium humate and after 60 mm evaporation from class A evaporation pan) in 3 locations of Ardabil in northwestern...

Author(s): Davoud Hassanpanah

January 2010

Molecular identification and food source inference of constructive plants, native to the Ophiocordyceps sinensis habitat

  Ophiocordyceps sinensis is a precious and effectual Traditional Chinese Medicine, native to Tibetan Plateau. Its development originated from parasitization of Hirsutella sinensis to Hepialus larvae, which was deemed to be of great importance including feeding food habit. Hereby in the present, study on food resource plants and their molecular identification technique were...

Author(s): Wei Lei, Hai Chen, Gu-ren Zhang, Shao-song Li, Qing-yun Peng, Xin Zhong and Xin Liu

January 2010

Fungal flora and deoxynivalenol (DON) level in wheat from Jeddah market, Saudi Arabia

  Deoxynivalenol (DON) is one of the least toxic trichothecenes, however, it is the most prevalent trichothecenes in human foods and its presence is an indicator of the possible incidence of other more toxic trichothecenes. This study aimed to explore the fungal flora along with the DON concentration in the collected wheat samples from Jeddah market to correlate between this flora and the detected DON. Whole...

Author(s): Al-Hazmi, N. A.

January 2010

Synergistic effect on co-pyrolysis of capsicum stalks and coal

  With the depletion of fossil fuel and the concern about environmental issues, the utilization of biomass resources has attracted increasing worldwide interest. The pyrolysis behavior of capsicum stalks and Baoji coal mixtures was investigated by TG-DSC. Results show that the thermal degradation temperature range of capsicum stalks was 290 to 387°C,...

Author(s): Yanqing Niu, Houzhang Tan, Xuebin Wang and Tongmo Xu

January 2010

Antimicrobial screening of Terminalia avicennoides and Acalypha wilkesiana

The antimicrobial activities of extracts of Terminalia avicennoides (Mexican tea) and Acalypha wilkesiana (copper leaf) were screened against Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Klebsiella, Salmonella typhi, Staphyloccus aureus and Candida albicans. The extracts showed a better activity against all organisms but T. avicennoides showed high activity against C. albicans. The antimicrobial...

Author(s): Akpomie, O. O. and Olorungbon, S.

January 2010

Cultivation of the oyster mushroom (pleurotus ostreatus (jacq.) p. kumm.) in two different agro-ecological zones of Pakistan

  The current study describes the possibility of small-scale production facility forPleurotus ostreatus under two different agro-ecological regions (Peshawar and Swat of Khyber Pukhtoon Khwa of Pakistan). Different growth parameters such as spawn running time, number of crops, stalk height, stalk diameter, cap size, fresh weight, number of production days and the interval in days between the time of...

Author(s): Hassan Sher , Mohammad Al-Yemeni and Kiramat Khan

January 2010

Oil content and physicochemical characteristics of some wild oilseed plants from Kivu region Eastern Democratic Republic of Congo

  Seeds were collected from Carapa grandiflora, Carapa procera, Cardiospermum halicacabum, Maesopsis eminii, Millettia dura, Myrianthus arboreus, Myrianthus holstii, Pentaclethra macrophylla, Podocarpus usambarensis, Tephrosia vogelii andTreculia africana from Kahuzi-Biega National Park and the surrounding areas in D.R. Congo. Oils were extracted using ethyl ether in...

Author(s): Kazadi Minzangi, Archileo N. Kaaya, Frank Kansiime, John R.S. Tabutiand Bashwira Samvura

January 2010

Application of Lavandula officinalis L. antioxidant of essential oils in shelf life of confectionary

  Chemically synthesized compounds such as butylated hydroxy-anisole (BHA) and butylated hydroxytoluene (BHT) are used as antioxidants in food products. The safety of some of these compounds have been a great concern. So, there is a great interest in finding antioxidants from natural sources for food. Lipids, known asLavandula officinalis L. in this work, harvested in the north of Iran was distilled in a...

Author(s): Heidar Meftahizade Hojat Moradkhani, Atefeh Fayazi Barjin and Behrouz Naseri

January 2010

Aluminum-induced testosterone decrease results in physiological and behavioral changes in male mice

  Recently, there has been much controversy on the role of testosterone on social and aggression behaviors. This work aimed to determine the effect of testosterone decrease, induced by aluminum exposure on the level of aggression. Male Swiss-Webster strain mice were classified into three groups. The first (control group) received distilled water, while the second and third groups were administrated 300 and 600...

Author(s): Gasem M. Abu-Taweel, Jamaan S. Ajaremand Hossam Ebaid,

January 2010

Cytotoxic, antibacterial activity and physico-chemical properties of some acid catalyzed Schiff bases

  Some novel Schiff bases have been derived using aromatic aldehydes and aromatic amine in ethanol under acidic conditions. The geometry of these Schiff bases was established using various spectroscopic methods like infrared (IR), nuclear magnetic resonance (NMR) and mass spectrometry. The conductivity studies reveal the non electrolytic behavior of Schiff bases. The synthesized Schiff bases were...

Author(s): Malik Amanullah, Sher Khan Sadozai, Wajid Rehman, Zonera HassanAbdur Rauf and Muhammad Iqbal

January 2010

Expression of human papillomavirus type 16 (HPV16) L1 protein in Pichia pastoris

  To investigate the production of human papillomavirus type 16 (HPV16) L1 structural protein in Pichia pastoris, the effect of different gene constructs and varying cultivation conditions on production levels were assessed. The highest volumetric production levels in P. pastoris were obtained with a human codon-optimised gene construct. This construct displayed both the lowest codon adaptation...

Author(s):   L. Kotzé, J. J. Smith, R. den Haan, W. H. van Zyl and J. F. Görgens  

January 2010

Traditional information and antibacterial activity of four Bulbine species (Wolf)

  Ethnobotanical survey of Bulbine Wolf, (Asphodelaceae) used for various treatment, such as, diarrhea, burns, rashes, blisters and insect bites, was carried out in the Eastern Cape Province of South Africa. Information on the parts used and the methods of preparation was collected through questionnaire which was administered to the herbalists, traditional healers and rural dwellers which indicated...

Author(s): R. M. Coopoosamy

January 2010

The gene expression of caspasses is up-regulated during the signaling response of Aedes caspius against larvicidal bacteria

  Our current knowledge on the key molecular mechanisms and cognate signaling transduction, by the d-endotoxin-mediated mosquitoticidal effects, associated with exposure to Bacillus thuringiensis (Bt) and Bacillus sphaericus (Bs), is limited. Moreover, this observed mosquitocidal activity that is related to program cell death is largely unknown. Therefore, in an attempt to answer...

Author(s): Abdulaziz A. Al-Roba, Mourad A. M. Aboul-Soud, Ashraf M. Ahmed, andAbdulaziz A.  Al-Khedhairy,

January 2010

Evaluation of biochemical changes in Clarias gariepinus adults exposed to aqueous and ethanolic extracts of Parkia biglobosa pods

  Clarias gariepinus adults were exposed to aqueous and ethanolic extracts of Parkia biglobosa pods (85 mg/L, 100 mg/L, 115 mg/L, 130 mg/L and 145 mg/L), respectively, in order to evaluate their effects on the biochemical parameters of exposed fish. There were significant increases (P < 0.05) in aspartate aminotransferase and alanine aminotransferase activities as well as in plasma glucose...

Author(s): Samson E. Abalaka, King A. N. Esievoand Shodeinde V. O. Shoyinka

January 2010

Length-weight relationships of nine fish species from Ologe Lagoon, Lagos, Nigeria

  This study describes the length-weight relationships of (LWR) of nine fish species from Ologe Lagoon, Lagos, Nigeria. A total of 1635 specimens were collected by local fishing gears from January, 2007 to December, 2007. The slope (b) values obtained for the nine fish species ranged from 2.5 to 3.2, and deferred significantly (p < 0.005) from 3, which indicates that the fish species have allometric growth....

Author(s): Kumolu-Johnson, C. A. and Ndimele, P. E.

January 2010

Bioinformatic analysis of Rp1 gene causing visual disparity in humans

  Retinitis pigmentosa (RP) is a group of inherited diseases that damage rod and cone cells located in human retina. A nonsense mutation R677X has been identified in RP1 gene which not only causes mRNA degradation but also results in truncated protein production leading towards visual disparity in humans. Secondary structure of RP1 gene was determined in order to elucidate the structural changes conferred due...

Author(s): Sana Zahra and Hamid Rashid

January 2010

Mapping and characterisation of the sorghum cell suspension culture secretome

  Here we reported the first secretomic study of sorghum (Sorghum bicolor), a naturally drought tolerant cereal crop. In this study, we used a gel-based proteomic approach in combination with mass spectrometry to separate and identify proteins secreted into the culture medium of sorghum cell suspensions, a first step towards understanding their functions during plant growth and development. Proteins secreted...

Author(s): Rudo Ngara and Bongani K. Ndimba

January 2010

Anti-hepatotoxic and anti-oxidant effects of extracts from Piper nigrum L. root

  The aim of this study was to investigate the effect of Piper nigrum L. root extracts on carbon tetrachloride (CCl4)-induced rat liver injury. Among the three different extracts (water, ethanol and chloroform extract), ethanol extract exhibits the highest hepatoprotective activity (p < 0.05). When using the ethanol extract at a dose of 120 mg/ kg to treat the...

Author(s): Xinpeng Bai, Weimin Zhang, Wenxue Chen, Wei Zong, Zhiyong Guo and Xiaoqin Liu

January 2010

In vitro cytotoxic and antioxidant properties of the aqueous, chloroform and methanol extracts of Dicranopteris linearis leaves

  The in vitro cytotoxic and antioxidant properties of the aqueous, chloroform and methanol extracts of the Dicranopteris linearis leaves were investigated in the present study. The cytotoxic effect was determined against the normal (3T3) and cancer cells’ lines (MCF-7, HeLa, HT-29, HL-60, K-562 and MDA-MB-231) using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium...

Author(s): Z. A. Zakaria, A. M. Mohamed, N. S. Mohd. Jamil, M. S. Rofiee, M. N. Somchit, A. Zuraini, A. K. Arifah and M.R. Sulaiman

January 2010

The antioxidant activity of wild medlar (Mespilus germanica L.) fruit, stem bark and leaf

  The medlar is an edible fruit. Modern medicine has recognized its healing properties in the treatment of some diseases. There is no scientific data in the literature about the antioxidant activity of methanol or aqueous extract of medlar fruit, leaf or stem bark. Antioxidant activities of these parts were evaluated by employing six test systems. Stem bark extract (aqueous and...

Author(s): Seyed Fazel Nabavi, Seyed Mohammad Nabavi, Mohammad Ali Ebrahimzadeh and Hossein Asgarirad