African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Article in Press

Analysis of production of biogas from peel of fruits and leaf of vegetable with cow dung as inoculants under anaerobic digestion

Biogas is a renewable source of energy that belongs to the category of biofuels. Anaerobic condition typically refers to a gas produced by the biological breakdown of organic matter in the absence of oxygen. Fruit and vegetable wastes (FVW) were evaluated from feed stalk materials for biogas production under anaerobic digestion. The biogas production and methane content of fruit and vegetable wastes digestion were...

Author(s):Massreshaw Assnakew Abebe

Article in Press

DNA extraction from silica gel-preserved common bean (Phaseolus vulgaris L.) leaves

Extraction of non-degraded and contaminant-free DNA from field specimen requires collection under liquid nitrogen which is not readily available in resource constrained laboratories in low and middle income countries (LMICs). A method of extracting DNA from silica gel-preserved common bean (Proteus vulgaris L.) leaves is presented. The method, which does not involve the use of phenol, chloroform or isoamyl alcohol also...

Author(s):Male Allan Ssekamatte, Kato Fred and Mukankusi Clare Mugisha

Article in Press

Biochemical characterization of a cellulase-free xylanase obtained from a newly isolated fungus, Mucor Mucedo, grown under optimized conditions

The study of the isolation, screening and identification of a xylanase-producing fungus, purification and characterization of xylanase was undertaken in order to produce the enzyme from a cheap source, saw dust, for possible industrial use. Eight fungal strains were isolated and identified from saw-dust out of which Mucor mucedo had the maximum xylanase production and it was selected as the best xylanase-producing strain....

Author(s):Adejumo, Adekunle Testimony, Ademakinwa, Adedeji Nelson, Ayinla, Zainab Adenike, Adejumo, Ayooade Lateef and Agboola, Femi Kayode

Article in Press

Marker-assisted backcrossing for associating nutritional quality to drought tolerance in barley

Backcross breeding enhanced by marker assisted selection is currently the most powerful method used to transfer one or a few genes controlling specific traits from one line into another. This method was used to cross naked barley variety, “Tombari” known as A, having high nutritional quality with hulled variety, “Giza131” called B, highly tolerant to drought. The objective of this work is to transfer the hulless variety...

Author(s):Amani Ben Naceur, Sameh Mnasri, Hatem Cheikh-M’hamed, Chedly Abdelly and M’barek Ben Naceur

Article in Press

Identification and mapping of quantitative trait loci associated with soybean rust (Phakopsora pachyrhizi) resistance in genotype UG 5

Soybean rust, Phakopsora pachyrhizi, is one of the most serious and widespread foliar diseases of soybean causing high yield losses world-wide. The objective of this study was to identify and map quantitative trait loci (QTLs) resistant to soybean rust in genotype UG 5. Ninety-seven F2 mapping plants, obtained from a cross between Wondersoya and UG 5, were used for this study. Quantitative trait locus analysis using QTL...

Author(s):Hailay Mehari Gebremedhn, Ulemu Mercy Msiska, Miesho Belay Weldekidan, Fentaw Abate Asmamaw, Akech Winnifred, Dramadri Isaac Onziga, Thomas Lapaka Odong, Patrick Rubaihayo, Phinehas Tukamuhabwa

Article in Press

Quali-quantitative characterization of the honey from Myracrodruon urundeuva allemão (Anacardiceae - Aroeira): macroscopic, microscopic, physico-chemical and microbiological parameters

The objective of the present study was to evaluate the external and organoleptic characters, microscopic, physico-chemical, chromatic and microbiological parameters of the honey from Myracrodruon urundeuva allemão (Anacardiceae - Aroeira) of the Jequitinhonha River Valley / Brazil, in order to create its Geographical Indication. In the first stage a data survey was carried out to characterize the production of honey in the...

Author(s):Eliane Macedo Sobrinho Santos, Hércules Otacílio Santos, Juliana Rezende Sá Miranda Gonçalves, Anna Christina Almeida, Igor Viana Brandi, Alexsander Rodrigues Cangussu, Janainne Nunes Alves, Ricardo Jardim Neiva, Grazielle Layanne Mendes Santos, Maria Izabel de Jesus Viana, Bruna Ruas Santos Araújo, Thalita Cordeiro Santos, Kattyanne Souza Costa

Article in Press

Uterotonic effect of aqueous extract of Launaea taraxacifolia Willd on rat isolated uterine horns

Launaea taraxacifolia is used by the traditional health practitioners in Sokoto to ease labour pains and augment labour thereby facilitating child birth. This study examined the effect of the aqueous extract of the whole plant on isolated uterus from non-pregnant rats pre-treated with stilbestrol and from pregnant rats in late gestation. This was compared to the effects of uterine contraction agonists, namely; oxytocin,...

Author(s):Adebisi, Mobolawa Iyabo and Alka, Hauwa

Article in Press

Comparison of four DNA extraction methods in Heliconia (Heliconia L.)

Molecular analysis in plants requires the use of high quality DNA. Currently, there are a myriad of DNA extraction protocols, which still seems that their applicability across different species is not straightforward. The aim of the present study was to evaluate four different cetyl trimethylammonium bromide (CTAB)-based DNA extraction protocols for Heliconia species from lyophilized fully mature leaves. A split-plot...

Author(s):Olvera S., Cortés-Cruz M., Contreras-Toledo A., Ortiz S., Avendaño-Arrazate C., Olivera A., Canul J. and Grajales M.

Article in Press

Phylogenetic analysis of bluetongue virus serotype16 based on genome segment 5 (encoding NS1)

Bluetongue virus (BTV) is the ‘type’ species of the genus Orbivirus within the family Reoviridae. The BTV genome is composed of ten linear segments of double-stranded RNA (dsRNA), each of which codes for at least one of ten distinct viral proteins. Phylogenetic analysis based on VP2, VP3, VP5, VP7 and NS3 gene has been advocated by different researchers around the world. However, very little information about the...

Author(s):Molalegne Bitew, Sukdeb Nandi, Chintu Ravishankar and Asit Sharma

Article in Press

The nutrient composition of three indigenous fruits in Botswana

Indigenous fruits play important roles in food security in rural and urban areas of many African countries. Of several indigenous grown in African countries, three fruits (Azanza garckeana, Grewia flava and Ximenia caffra) were selected for the study. The edible portions were analyzed for vitamin C, titratable acidity, dry matter, ash, crude protein, acid detergent fibre (ADF), acid detergent lignin (ADL), neutral...

Author(s):Olekile Tibe and Joel L. Seolwane

Article in Press

Preliminary study on rice straw degradation using microbial inoculant under shake flask condition

Abundance and unmanaged agriculture residues lead to unutilized resource waste and environmental pollution. Application of microbial technology to manage agriculture waste could produce value-added product. A preliminary study on biodegradation of rice straw using different potential microorganisms was tested under controlled environment. Three different inoculant cultures were used to observe their efficiency in rice...

Author(s):Nurul Ain Abu Bakar, Mohammad Hariz Abdul Rahman, Nur Alyani Shakri, Syuhaidah Abu Bakar and Abhar Abdul Hamid

Article in Press

Genetic diversity of Aegilops tauschii Coss. among Chinese populations using single sequence repeat (SSR) markers and morphological traits

Aegilops tauschii Coss. (2n = 2x= 14, DD) is a problematic weed and contributes to the D genome in common wheat. Genetic diversity among 40 populations of China was evaluated by microsatellites marker and morphological traits. For dry biomass weight, there was variation among the populations, while for plant height, there was a high variation (15.8%) among the populations. We determined 27 alleles by using eight primers....

Author(s):Adeel Abbas, Haiyan Yu, Hailan Cui, Huilin Yu and Xiangju Li

Article in Press

Superoxide dismutase activity and jasmonic acid during in vitro ex vitro transition of pineapple (Ananas comosus (L.) Merr.) micropropagated plantlets

Recent agriculture is characterized by intensive and cleaning productions, which need seeds with high quality in large quantities bonded by in vitro culture labs. Nevertheless in vitro ex vitro transition and during acclimatization occur losses due to the death of plantlets unable to survive this abiotic stress. Reactive Oxygen Species production during jasmonic acid-induced changes previous transition was demonstrated....

Author(s):González-Olmedo, J. L., Garza-García, Y., Mbogholi, A., Rodríguez-Escriba, R.C., Aragón, C. E., Rodríguez, R.

Article in Press

Investigation of haematological changes following Kolaviron intervention in Nevirapine therapy: An exerimental animal model

This study evaluated the haematotoxic effects of Nevirapine and the role of Kolaviron, a potent antioxidant from Garcinia kola as an adjuvant. Forty eight adult male Sprague-Dawley rats were used in this study. The animals were divided into eight groups, A-H (with 6 rats per group). Group A animals received normal saline as the control; Group B were given Nevirapine (1.54 mg/kg); Group C received Kolaviron (200 mg/kg);...

Author(s):Ajayi Sunday A., Azu Onyemaechi O., Naidu Edwin C. S., Naidu Jesse S., Komolafe Omobola A. and Ofusori David A.

Article in Press

Biocontrol activity of chitinase from marine sponge Callyspongia diffusa associated Pseudomonas fluorescens BCPBMS-1

Marine sponge Callyspongia diffusa associated with Pseudomonas fluorescens BCPBMS-1 was isolated from Madapam Coast, Tamil Nadu, India and screened for chitinase production. Chitinase was tested for its biocontrol activity against phytopathogenic fungi. Chitinase is an enzyme capable of hydrolyzing chitin, an insoluble polysaccharide present in the cell wall of higher fungi, insect gut walls and nematodes. Chitinase was...

Author(s):V. Vasanthabharathi and S. Jayalakshmi

Article in Press

Isolation of cellulase producing Aspergillus unguis UCSC324 and characterization of cellulase

Bioengineering of cellulolytic enzymes with enhanced catalytic efficiency and thermostability is important in the commercialization processes. This study describes the isolation of efficient cellulase producing fungi and determination of the kinetic properties of the crude cellulase. Among the fungal strains isolated from cow dung, hot rice water, water used in autoclave and decaying coconut wood, the strains growing on...

Author(s):Mohanappriya Shanmugarajah & Ranganathan Kapilan

Article in Press

Evaluation of genetic diversity of pearl millet (Pennisetum glaucum L.) landraces from West Africa using microsatellite markers

Pearl millet (Pennisetum glaucum L.) is an important staple food crop, especially in arid and semi-arid regions of West Africa. However, the genetic diversity of pearl millet in these regions is poorly understood, and yet it is useful in identification of landmarks for in situ germplasm conservation and breeding programs, particularly in establishment of heterotic groups. Therefore, the aim of this study was to assess the...

Author(s):Mary N. Onsarigo and Dionysious K. Kiambi

Article in Press

Regeneration of Washington navel sweet orange (Citrus sinensis L. Osb.) by in vitro organogenesis from floral and vegetative explants

For viral sanitation strategy in Tunisia, citrus accessions from Washington navel (Citrus sinensis L. Osb.) variety were investigated for regeneration throughout direct and indirect in vitro organogenesis. In this study, either floral (style/stigma and ovaries) or vegetative organs (leaves, cotyledons, epicotyls and hypocotyls) were cultured into Murashige and Skoog (MS) culture media that were supplied with five different...

Author(s):Nebiha METOUI, Fethia DHAOUADI and Dorsaf YAHIAOUI

Article in Press

Simple sequence repeat (SSR) marker-based DNA fingerprinting of some varieties of rice (Oryza sativa L.) released in Nigeria

Fingerprinting with molecular markers allows precise, specific and rapid variety identification. In this study, 13 out of 45 screened simple sequence repeat (SSR) markers were used to fingerprint 27 rice varieties (22 are commercially released while five are suspected duplicates from the hands of marketers with different names) in Nigeria. The SSR primer pairs (13) were polymorphic and generated a total of 81 allele...

Author(s):Ishaq, M. N., Ehirim, B. O., Nwanyanwu, G. C. and Abubaka, R. I.

Article in Press

Biotechnological tools for detection, identification and management of plant diseases

Detection and identification of plant pathogens is one of the most important strategies for sustainable plant diseases management. For this reason, the availability of fast, sensitive and accurate methods for detection and identification of plant pathogens is increasingly necessary to improve disease control decision making process. In other words, new technologies and improved methods with reduced/fair cost and improved...

Author(s):Tibebu Belete and Nuh Boyraz

Article in Press

Isolation of Ursolic Acid from the Leaves of Ocimum lamiifolium collected from Addis Ababa Area, Ethiopia

Ocimum lamiifolium is a well known medicinal plant in Ethiopia. Fresh leaves of the plant are squeezed and sniffed to treat colds and coughs and as an eye rinse for eye infections, while crushed leaves are used to arrest nose bleeding. In this study phytochemical investigation on the methanol extracts of this plant collected from Addis Ketema subcity, Addis Ababa resulted in the isolation of a triterpene, namely U

Author(s):Girma Birhanu

Article in Press

Thiourea medium supplementation improves salinity tolerance of hybrid maize: Evidence from whole plant and cellular level studies

These experiments were conducted to evaluate the role of thiourea on accumulation of osmoprotectants and antioxidants in the salt stressed maize roots and callus of maize hybrids. Medium supplementation of 400 µM thiourea was applied on two maize hybrids; 33M15 and DK6789. The maize hybrid 33M15 performed better than DK6789. Fifteen days older plants and immature embryo were subjected to medium supplemented with thiourea...

Author(s):Tayyaba Sanaullah, Ali Noman, Muhammad Aqeel, Noreen Khalid, Abdul Wahid and Bushra Sadia

Article in Press

Zinc biofortification of wheat as influenced by zinc fertilization

Zinc (Zn) fertilization is an effective agronomic tool for Zn biofortification of wheat to overcome Zn deficiency in humans. Zinc deficiency in humans caused by inadequate dietary intake is a global nutritional problem. Biofortification of wheat with Zn is, therefore, an important challenge and a high-priority research work. In the present study, a field experiment was conducted to explore the impact of soil and foliar Zn...

Author(s):Mohammad Delwar Hossain, Motarab Hossain, Ahmad Khairul Hassan and Ahmad Ainuddin Nuruddin

Article in Press

Haplotype analysis of Ethiopian bread wheat (Triticum aestivum) cultivars and elite lines for yellow rust resistance genes using linked molecular markers

Yellow rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat in Ethiopia. Poor knowledge of resistance genes deployed in Ethiopian wheat cultivars is one major factor for recurrent epidemics of rust diseases. Molecular marker based gene identification showed the presence of Yr1, Yr4, Yr6, Yr7, Yr9, Yr10, Yr17 and Yr18 in various frequencies, whereas Yr8 was not detected in any...

Author(s):Woubit Dawit, Ayele Badebo, Bekele Hunde, Daniel Kassa and M. S. Röder

Article in Press

Organic matter and N removal performance of a recirculating aquaculture system (RAS) for rainbow trout culture with aerobic-anaerobic reactor, three phase airlift fluidized bed reactor and filtration units

The performance of an aerobic three phase fluidized bed reactor for the treatment of surface water in the culture tanks effluent, and an anoxic-anaerobic reactor for the bottom effluent of the rearing tanks treatment is show in this study. The main objective of the study was to evaluate the organic matter and nitrogen removal efficiency in a recirculating aquaculture system (RAS) for intensive rainbow trout laboratory...

Author(s):Gloria Cárdenas Calvachi and Iván Sánchez Ortiz

Article in Press

Genetic dissection of quantitative trait loci (QTL) associated with agronomic traits in an interspecific rice population evaluated under drought and irrigated environment

Numerous studies have been conducted in rice to map quantitative trait loci (QTLs) associated with different agronomic traits. However, only a few of these studies have used interspecific mapping populations. Here, QTLs associated with tiller numbers, flowering time, plant height, and grain yield in an interspecific rice population were mapped. The population was evaluated under managed water-stressed (drought) and optimal...

Author(s):Marie Noelle Ndjiondjop, Peterson W. Wambugu, Mamadou Cissoko, and Kassa Semagn

Article in Press

Comparison of meristem culture and heat therapy to clean garlic (Allium sativum L.) infecting virus in Ethiopia

This experiment was aimed to detect garlic infecting virus for three improved garlic varieties (Bishoftu Nech, Tseday and Kuriftu) collected from different garlic production sites of Ethiopia. Garlic cloves were planted in screen house at Biosciences Eastern and Central Africa-International Livestock Research Institute Hub Nairobi, Kenya in 2014. Reverse Transcription polymerase chain reaction (*RT-PCR) technique was...

Author(s):Abel Debebe

Article in Press

Phenotypic and genotypic characteristics of cowpea rhizobia from soils of Ethiopia

Phenotypic and genotypic characteristics of cowpea rhizobia indigenous to soils of Ethiopia are unknown. Forty indigenous cowpea rhizobial isolates were collected from cowpea-growing areas of the country and were characterized for their growth and genetic properties. Based on their cultural characteristics, the isolates were categorized into fast (FG), slow (SG), and extraslow-growings (ESG). The FG, SG, and ESG isolates...

Author(s):Girmaye Kenasa, Fassil Assefa and Mussie Y. Habteselassie

Article in Press

In vitro propagation of the wild medicinal plant, caper (Capparis spinosa L.)

Micropropagation of caper (Capparis spinosa L.) was done using wild leaves from Libya. High percentage (95%) of callus induction was obtained from Murashige and Skoog (MS) medium supplemented with 1.2 mg l-1 2,4-D + maltose. The effect of various concentrations (0.0, 0.5. 1.0 and 2.0 mg l-1) of cytokines (BAP, 6-γ-γ-[Dimethylallylamino]-purine 2ip, Kin and Zea) supplemented with MS medium was evaluated for callus...

Author(s):Adel M. Elmaghrabi, Elmundr Abughnia and Salem Hamoud

Article in Press

Degradation kinetics of 2,4-dichlorophenoxyacetic and atrazine by Trametes versicolor (L.:Fr.) Pilát

The degradation kinetics of different sources of 2,4-dichlorophenoxyacetic (2,4-D) and atrazine by the natural strain Mo008 of the basidiomycete fungus Trametes versicolor (L.:Fr.) Pilát was studied, knowing that in this process the strain used produces an enzyme complex composed of manganese peroxidase (MnP), lignin peroxidase (LiP) and laccase, and the response on the global consumption rate and yield is dependent on the...

Author(s):Ernesto Hernández Mendieta, Dagoberto Guillén Sánchez, Iran Alia Tejacal, Victor López Martínez, María Andrade Rodríguez, Edgar Martínez Fernández, Manuel Huerta Lara, Catalina Rubio Granados

Article in Press

Role of Toll-like receptor 4 in regulating nucleotide-binding oligomerization domain2 (NOD2)-mediated inflammatory responses through Aspergillus fumigatus conidia stimulation in RAW264.7 macrophages

Nucleotide-binding oligomerization domain2 (NOD2) of the NOD-like receptors (NLRs) and Toll-like receptor4 (TLR4) of the TLRs are major pattern-recognition receptors. They sense different microbial pathogens and play important roles in innate immunity. Current in vivo and in vitro studies have shown that TLR4 and NOD2 act in anti-Aspergillus fumigatus infection. Herein, the cross-interaction between membrane-bound TLR4 and...

Author(s):MA Guang-qiang, WU Su-fang, LUO Hong-dan, XIE Wei-hua, LONG Kai, SU Ming-sheng and XIE Xiao-mei

Article in Press

Chemical Composition of Cyperus esculentus nut and Phoenix dactylifera fruit

This study investigated the chemical composition of Cyperus esculentus nut and Phoenix dactylifera fruit. The nuts and fruits were purchased as commonly sold in Wukari, Nigeria. They were cleaned, sorted for healthy parts, sun-dried and milled into fine powder. The AOAC, AAS and GC-MS methods were adopted for the proximate, mineral and phytochemical analysis respectively. The results show that Cyperus esculentus nut is...

Author(s):Chinedu Imo, Friday O. Uhegbu, Kayode A. Arowora, Chukwuma S. Ezeonu, Ifeoma J. Opara, Emmanuel E. Etim and Chigozie J. Anigbo

Article in Press

The oxidative stability of linolenic fatty acid in farmed rainbow trout (Oncorhynchus mykiss) under different storage conditions in Isfahan City of Iran

The aim of this study was to investigate the effect of different storage conditions in market (ice box, glassy refrigerator, normal refrigerator without light (natural and electric power) and freezer) on alpha linolenic acid (ALA), a nutritional value of rainbow trout (Oncorhynchus mykiss). To investigate the effect of storage duration on fatty acid quality, samples stored after 1, 3 and 5 days in refrigerator and those...

Author(s):Lakzadeh, L., Mirmohammadi, M., Amouheidari, M. and Bavarian, A.

Article in Press

pression, Purification and On-Column Refolding of Recombinant S-layer Protein from L. acidophilus ATCC 4356

One of the most significant challenges in biotechnology is technological utilization of self-assembled systems. Surface (S-) layer technology is advanced due to the intrinsic property of subunits to reassemble into two-dimensional arrays. Lactobacillus acidophilus ATCC 4356 composed of S-layer protein (SlpA) subunits which are organized into regular arrays. Herein, S-layer gene (slpA) of L. acidophilus was cloned in...

Author(s):Elaheh Sadat Hosseini , Rouha Kasra Kermanshahi , Saman Hosseinkhani and Mahboobeh Nazari

Article in Press

Scanning electron microscope studies of pollen of herbaceous species of Amritsar, North-Western Punjab, India

Palynological studies in combination with conventional morphology have a significant role in solving problems relating to plant systematics. Scanning electron microscope (SEM) is a useful tool for palynological studies with increased accuracy and precision. The pollen size, shape and details of sculpturing of the exine are of taxonomic value. The main objective of the present study was to provide a detailed morphological...

Author(s):Harpreet Kaur and Avinash Nagpal

Article in Press

Characterisation of volatile compounds and flavour attributes of Lablab purpureus bean accessions

The Lablab bean (Lablab purpureus) has potential of being an outstanding resource for human food and animal feed in tropical agricultural systems. The bean is however grossly underutilized due anti-nutritional factors, which may affect its nutritive value and organoleptic properties. In this study, twenty-four (24) lablab bean accessions were assayed for the volatile compounds and sensory flavor characteristics to...

Author(s):Lablab purpureus, flavour, taste, volatile compounds.

Article in Press

Merzouga Sand Dunes Actinobacteria: antimicrobial potential and taxonomic diversity

Merzouga sand dunes were among the Moroccan extreme environments. Actinobacteria from this under investigated desert habitat were isolated and their antimicrobial activity was evaluated. One hundred sixty three Actinobacteria isolates were isolated from sand collected from several dunes and screened for their antibacterial and antifungal activities. To test the antimicrobial effect of isolates, four microorganisms known as...

Author(s):Lahcen Ouchari, Amal Boukeskasse, Brahim Bouizgarne and Yedir Ouhdouch

Article in Press

Clausenidin upregulated p53 and caused apoptosis in HT-29 tumor cell lines

Introduction: Clausenidin is a pyranocoumarin majorly found in medicinal herbs of the Rutaceae family used to treat cancer patients locally in Asia. The compound is presumed to have anti-cancer cell effect but its exact mechanism of action is still unknown. Aim: The study aimed to evaluate the effect of pure clausenidin on p53-mediated apoptosis as well as other cell death pathways in colon cancer (HT-29) cell lines....

Author(s):Peter M Waziriab, Rasedee Abdullahc, Rosita Roslia, Abdul Rahman Omard, Ahmad Bustamam Abdula, Nur Kartinee Kassime, Ibrahim Malamia, Imaobong C Ettia, Palanisamy Arulselvand and Ashwaq Shakir Al-Abboodia.

Article in Press

Protocol optimization of in vitro propagation of an endangered endemic Ethiopia medicinal plant, Echinops kebericho (Mesfin), using shoot tip

Echinops kebericho (Mesfin)isthe most important and critically endangered endemic Ethiopian medicinal plant. This plant is threatened due to over harvesting of its roots for medicinal purpose, and from seed viability and seed germination problem. The aim of this research was to build up an efficient in vitro propagation protocol for E. kebericho using shoot tip explants. Shoots excised from in vitro germinated seedlings...

Author(s):Muluken Enyew; Tileye Feyissa

Article in Press

Development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli, a food-borne pathogen

This study presents the development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli. Bacteriophage is very specific to host bacterial cell also known as parasite for bacteria. The Electrochemical Quartz Crystal Microbalance (EQCM) is a very sensitive device that measures a mass change per unit area by measuring the change in resonant frequency of a quartz crystal. A...

Author(s):Vipin Singh and Pranay Jain

Article in Press

Morphological diversity studies among sugarcane (Saccharum spp.) somaclones generated through callus culture

Callogenesis is one of the tools in sugarcane tissue culture for generating agronomically significant variation. Tissue culture derived variations are known as somaclonal variation. The study was carried out to investigate the effect of MS media supplemented with 1.0 mgL-1 of NAA (naphthalene acetic acid) and 2.0, 2.5, 3.0, 3.5 and 4.0 mgL-1 of 2,4-D (2,4-dichlorophenoxy acetic acid) on somaclonal variation in in vitro...

Author(s):Richard Wekesa, Justus M. Onguso, Bernard A. Nyende and Leonard S. Wamocho

Article in Press

Biodegradation of crude oil by Bacillus species isolated from different sources

The consequences of oil pollution to the environment and human health as a result of exploration activities, transportation and usage of petroleum compounds, has become a serious challenge to environmental scientists. This study evaluates the potential of using Bacillus subtilis in biodegradation of petroleum compounds. Ten Bacillus strains from non-petroleum contaminated sites were identified on the basis of microscopy...

Author(s):Mohammed Inuwa Ja’afaru, Chimezie Godwin Azuchukwuene and Aisha Sa’id Shittu

Article in Press

Analytical quantification of copper in frogs (Rana tigrina) found from various aquatic habitats

This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina captured from industrial site and...

Author(s):Muhammad Nasir, Tariq Mahmood Ansari, Ghulam Yasin, Aleem Ahmad Khan and Muhammad Shoaib

Article in Press

Marker-assisted pyramiding of Xa21 and Xa7 genes conferring resistance to bacterial leaf blight in indica cultivar Bacthom7

Bacterial leaf blight (BLB) of rice is one of the most destructive diseases affecting rice fields. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of BLB. Two BLB resistance genes, Xa21 and Xa7, were transferred into the susceptible indica cultivar Bacthom7 (BT7) by using marker-assisted selection with markers pTA248 for Xa21 and ID7 for Xa7. Improved BT7 lines carrying the two resistance genes were inoculated with...

Author(s):Nguyen Thi Thu#, Vu Hong Quang#, Mai Van Tan#, Vu Đuc Lam, Nakano Toshitsugu, Nguyen Thi Hue, Nong Thi Hue, Nguyen Quoc Trung, Nguyen Viet Long, Nguyen Van Hoan and Vu Van Liet

Article in Press

Psychrotrophic proteolytic bacteria and their characterization from landfill soil under temperate climatic conditions of Kashmir Himalaya

During cold season in Kashmir valley, the decomposition of municipal solid waste slows down due to slowing down of microbial activity as a result, the waste gets piled up and its management becomes a greater challenge. Psychrotrophic proteolytic bacteria play an important role in the degradation of protenaceous materials of the waste stream under low temperatures that produces nitrogen sources for rapid functioning of...

Author(s):Basharat Hamid, Arshid Jehangir and Zahoor Ahmad Baba

Article in Press

Holistic plant regeneration protocol from callus cultures of Salvia moorcroftiana, a medicinal herb

Salvia moorcroftiana is a medicinal herb of the family Lamiaceae. The objective of this study was to develop an in vitro protocol for the rapid propagation of this species. Organogenesis by means of callus culture was carried out on Murashige and Skoog medium by the transfer of callus from an induction medium supplemented with α-naphtalenacetic acid (NAA, 1.0 mg/L) to regeneration medium containing 6-benzyladenin (BA, 1.0...

Author(s):Tour Jan, Ali Hazrat ,Rashid Azam, Arshad Ali Mohammad Nisar, Nasrullah Khan and Muhammad Asif Nawaz

Article in Press

Bioremediation potentials of Sunflower (Tithonia diversifolia) and pseudomonas species in soil contaminated with lead and zinc.

This study was conducted to assess the ability of sunflower (Tithonia diversifolia) inoculated with Pseudomonas species to remediate soils contaminated with heavy metals (lead and zinc). The contaminants were added to 20 kg soil in pots as lead nitrate (Pb (NO3)2) and zinc nitrate (Zn (NO3)2); 400 mg/l metal concentrations were added to 20 kg soil in pots. The experimental design was a randomized complete block design...

Author(s):Elizabeth Temitope Alori, Abiodun Joseph, Ojo Timothy Vincent Adebiyi, Ajibola Patrick Aluko and Chidera Onyekankeya

Article in Press

Antibacterial saponins from the leaves of Polyscias fulva (Araliaceae)

Saponins are a major family of secondary metabolites that occur in a wide range of plant species. Bioassay - guided fractionation of extract of the leaves of Polyscias fulva led to the isolation of three known saponins named, 3-O-α-L-arabinopyranosyl-hederagenin (1), 3-O-[α-L-rhamnopyranosyl(1-2)-α-L-arabinopyranosyl]-hederagenin (2) and...

Author(s):Winnie Cherotich Maritim, Isabel Nyokabi Wagara and Josphat Clement Matasyoh

Article in Press

Genetic variability, heritability and genetic advance for green pod yield and yield related traits in chili (Capsicum annum L.) accessions in Ethiopia

A field experiment was conducted to study the genetic variability, heritability and genetic advance for 12 quantitative characters in chili (Capsicum annum L.) accessions in Ethiopia, during the off-season period of 2012/2013 under flood irrigation at the Adet Agricultural Research Centre field. The experiment was conducted using Randomized Complete Block Design with three replications and a total of 25 accessions and four...

Author(s):Tadesse Tilahun, Yohannes Petros and Yigzaw Dessalegn

Article in Press

Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species

This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae: Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in 15 2-L column photobioreactors (PBR) (three per species). Growth and specific growth rates for the mixotrophic>autotrophic cultivation of the species were determined. Oil was extracted from the dried microalgae species at temperatures...

Author(s):Felix Uzochukwu Asoiro, Wilfred Ifeanyi Okonkwo, Clement Onyeaghala Akubuo, Nkechinyere O. Nweze and Cosmas Ngozichukwu Anyanwu

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