African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Expression, Purification and testing of Zinc Metalloproteinase Aureolysin as Potential Vaccine Candidate against Staphylococcus aureus

Article in Press

Staphylococcus aureus (S. aureus) is a versatile bacterium which exhibits multiple antibiotic resistances. To ameliorate the undesirable diseases causing potential, there is a need to design a protective vaccine capable of stimulating immune response against this pathogen. In a similar study in our laboratory, reverse vaccinology approach was used to nominate potential vaccine candidate genes against S. aureus. Zinc...

Author(s):Samar Solyman, Mohamed Reda and Amro Hanora

Role of Microorganisms in Biodegradation of Food Additive Azo dyes: A Mini Review

Article in Press

Food additives Azo dyes are synthetic compounds added to foods to impart color and improve their properties. Some azo dyes have been banned as food additives due to toxic, mutagenic, and carcinogenic side effects. Long exposure to foods containing azo dye leads to chronic toxicity. Some microorganisms are capable to degrade these dyes and convert them to aromatic amines. In human body, microbiota can play a vital role in...

Author(s):Fatimah Alshehrei

Improvement of the key flavour compounds of fermented garlic by fermentation process optimization

Article in Press

Flavour compounds in fermented garlic were identified by physicochemical analysis and gas chromatography (GC) after pre-treatment. Reducing sugars, acids, polyphenols and 5-hydroxymethyl furfural (5-HMF) were detected in fermented garlic, and 5-HMF was identified to be the key aroma compounds. To further improve the flavour quality of fermented garlic, the influence of pre-treatment method, fermentation temperature and...

Author(s):Yunwei Leng, Shuangshuang Huang, Bing Chen

Quantification of Indole-3-acetic-acid from Bambusa tulda Roxb. seedlings using high performance liquid chromatography

Article in Press

Indole-3-acetic acid (IAA) is an important plant growth regulator. As the very first endeavor, we aimed at extracting and quantifying IAA from seedlings of Bambusa tulda and evaluate its bioactivity. The extraction of IAA was performed in organic solvent followed by sample evaporation and TLC with a mobile phase composed of chloroform, methanol and formic acid (77:22:1 v/v) in isocratic mode. The extract obtained from...

Author(s):Bishnu Maya K.C., Dhurva Prasad Gauchan Sanjay Nath Khanal and JanardanLamichhane

Investigation of lipid production and fatty acid composition in some native microalgae from Agadir region in Morocco

Article in Press

Microalgae are a promising alternative source to produce biofuels and bio-products, as they consume carbon dioxide to grow, multiply quickly, and can be cultivated in domestic and industrial wastewater. The efficiency of microalgae-based biodiesel industry depends on the selection of appropriate strains with best lipid content yields and adequate fatty acids composition. The aim of this study was to select a potential...

Author(s):Youssef Mir, Zahra Brakez, Yassine El Alem and Lhoucine Bazzi

Production of Bio-ethanol from lignocellulosic substrates by using Saccharomyces cerevisiae and Bacillus macerans

Article in Press

Production of bio-ethanol from ligno-cellulosic substrates is one way to reduce the consumption of crude oil and environmental pollution. The main objective of this study was to produce bio-ethanol from different ligno-cellulosic substrates by using Saccharomyces cerevisiae and Bacillus macerans. Substrates were collected from Debre Tabor town and cut into smaller pieces, air-dried, and ground to a fine powder. The powder...

Author(s):Tolossa Duguma, Hirpasa Teressa, Aynias Seid, Eyob Kasaye

Brewer’s residues and cocoa pod shells as a substrate for cultivation of Pleurotus ostreatus CCIBt 2339 and enzymes production

Article in Press

This work investigated the best composition of a substrate for cultivation of Pleurotus ostreatus CCIBt 2339 using brewer's spent grain (SG) and cocoa pod husks (CP) complemented with hot trub (HT) and/or residual brewer’s yeast (RY). The residue HT was detrimental for cultivation and a substrate composition (%, w/w) of 58 % SG + 40 % CP + 2 % RY – with a C/N ratio of 25.77 g/g – resulted in the best values of biological...

Author(s):Carolina Fernandes Pereira, Antônio Zózimo de Matos Costa, Givaldo Rocha Niella, José Luiz Bezerra, Ana Paula Trovatti Uetanabaro, Elizama Aguiar-Oliveira

Symptoms; Epidemiology and Diagnosis: A mini-review on coronavirus

Article in Press

There is worldwide concern about the rapid spread of the Covid19 (COVID-19) pandemic which now affects almost every country in the world. Generating accurate diagnosis of COVID-19 by testing hundreds of thousands of people per day, and the search for effective therapeutics and vaccines are currently the focus of intense research in large numbers of companies and academic institutions around the world. This review will...

Author(s):Abdulkarim S BinShaya, Nahed Alharthi, Mohamed Bamaga, Hajir Alsaihati, Awal Zaki, Adnan Alwatban, Fahad Aldakheel, Aysha Alanazi, Abdulaziz Alanazi, Amani F. Alanazi, Arwa F. Alanazi, Faris Q.B. Alenzi

Comparative study of maggots’ production in brewery wet grains, dung and rumen content of cattle with or without addition of attractant

Article in Press

The objective of this study was to compare maggots’ production in different substrates following an experimental design of six treatments (S1 (1000 g of brewery wet grains - BWG); S2 (900 g of BWG and 100 g of blood); S3 (1000 g of dung); S4 (900 g of dung and 100 g of blood); S5 (1000 g of rumen content) and S6 (900 g of rumen content and 100 g of blood)) repeated trice. 121 flies were collected and identified and 5 days...

Author(s):Gilbert Comlan ZOUGOU TOVIGNON, Abdourhamane Ibrahim TOURE, Cédric SIMA OBIANG, Arsène Valery MBOKO, Férence Ndzani MATUMUINI, Taffarel Juani MEKOSSANGOYE NDOUNGA, Kady Divine MOUSSOUNDA GASSAMA, Frédéric Christol NGANSOP NONO, Joseph-Privat ONDO, Louis Clément OBAME ENGONGA, Franck MOUNIOKO, Fernand TENDONKENG, Emile MIEGOUE, Benoit BOUKILA, Etienne PAMO TEDONKENG

First report of mortality in Phoenix canariensis associated with subgroup 16SrIV‐D phytoplasmas in Coahuila, Mexico

Article in Press

In 2015, a disease of unknown origin appeared in Torreon, Coahuila, in the Northeast of Mexico, causing great mortality in Phoenix palms, especially in Phoenix canariensis. Until early 2019, around 1300 palms died from this disease. The aim of this study was to determine its etiology. The symptoms registered in affected palms were similar to those described for Texas Phoenix Palm Decline (TPPD). Phytoplasmas were detected...

Author(s):Pablo José Palma-Cancino, Carlos Oropeza, María Narváez, Germán Nic-Matos, Yasmín Chew-Madinaveitia, Aurelio Pedroza-Sandoval, Arturo Gaytán-Mascorro and José Alfredo Samaniego-Gaxiola

Analysis of the degradation of polyethylene, polystyrene and polyurethane mediated by three filamentous fungi isolated from the Antarctica

Article in Press

Plastic polymers are petroleum-derived synthetic materials that have multiple uses in everyday life, but their excessive production has led to the accumulation of approximately 1,000 million tons of residues, causing negative ecological impacts. This study analyzed the biological degradation in liquid medium of polyurethane, polystyrene, and polyethylene samples by filamentous fungi isolated from Antarctica. The plastic...

Author(s):Rodrigo Oviedo-Anchundia; Daynet Sosa del Castillo; Jaime Naranjo-Morán; Nora Francois; José Álvarez-Barreto; Alejandro Alarcón; Jhonny Saulo Villafuerte; Milton Barcos-Arias

Quality Evaluation of Low Free Fatty Acid and High Free Fatty Acid Crude Palm Oil and Variation of Total Fatty Matter and Fatty Acid composition in Nigerian Palm Oil

Article in Press

The quality of Palm oil determines its consumer and market acceptability and price, and this correlates directly to its Moisture (%), Free Fatty Acid (% FFA) and Total Fatty Matter (%TFM) content. Therefore, businesses utilizing palm oil for production are very particular about these quality parameters. The aim of the present study was to evaluate the quality of Low free fatty acid (LFFA) and high free fatty acid (HFFA)...

Author(s):IGILE GO, IWARA AI, EKPE OO, ESSIEN NM, EGBUNG GE

Antimalarial potential and protective properties of Azadirachta indica (Dongoyaro) ethanolic stem bark extract in mice infected with Plasmodium berghei NK65

Article in Press

The study determine the in vivo antiplasmodial potential and protective properties of Azadirachta indica (Dongoyaro) ethanolic stem bark extract in mice infected with Plasmodium berghei NK65. The GC-MS, qualitative, quantitative and antioxidant scavenging activities of the stem bark extract of A. indica were determined using standard procedure. The acute toxicity and in vivo antiplasmodial activities of the extract were...

Author(s):Momoh Johnson Oshiobugie, Adeniyi Michael Olaniyi and Aderele Oluwaseun Raphael

New Elite Sugarcane Varieties, 'Tafach Shenkora' and 'Kay Shenkora' , for Commercial Production in Ethiopia

Article in Press

'Tafach Shenkora' and 'Kay Shenkora' sugarcane (Saccharum spp.) are the names assigned to sugarcane varieties with accession numbers Acc. 43 and Acc. 139, respectively. They were developed by Sugar Corporation, Research and Development and were registered and released in June 2019 by the National Variety Releasing Committee of Ethiopia to growers in three sugar estates in Ethiopia. 'Tafach Shenkora' and 'Kay...

Author(s):Esayas Tena, Firew Mekbib, Abebech Seife, Zinaw Dilnesaw and Feyissa Tadesse

Molecular Characterization, Optimization of Polyhydroxybutyrate (PHB) production using agro-wastes as substrate by Bacillus cereus

Article in Press

Non biodegradability of plastics has discouraged its use and hence alternative eco friendly polyhydroxybutyrates have gained popularity. The use of cost effective and easily available substrates in the production of polyhydroxy butyrate (PHB) enhances its application and further reduces its cost and hence PHB can be efficiently used instead of plastics. The present work was performed to study the optimum production of...

Author(s):Jasmine R, Hannah S

Isolation and characterization of lactobacilli in raw milk derived whey and its potential use as probiotic

Article in Press

The main purpose of this study was to isolate lactobacillus from raw milk derived whey and to determine its potential use as probiotics. Fifteen samples of milk were obtained and tagged (LS1 to LS15) from a homogeneous group of Holstein cows and later at our laboratory the milk was processed to obtain whey, which was inoculated in MRS medium where we got 30 bacterial isolates and selected those who were bacilli gram...

Author(s):Guel-García G. Paulina, Rodríguez Castillejos Guadalupe C., Hernández Mendoza José L.

The adults of Anthonomus grandis as disseminator of Fusarium chlamydosporum and use of oregano extracts for their control

Article in Press

The Cotton Weevil is an insect that causes considerable losses to the cotton industry. Its larvae grow inside the cotton bolls, damaging them. It enters diapause during the winter and becomes active again in the first months of the year. Pheromone traps can be used to estimate their populations and implement control measures. This work analyzes samples of weevil populations collected in traps placed on mexican...

Author(s):Jesus DiCarlo Quiroz Velasquez; J. Luis Hernandez-Mendoza, Guadalupe Rodriguez Castillejos; Gerardo Sanchez Ramos; Cristian Lizarazo Ortega; Jesus Gerardo Garcia Olivares

GENETIC DIVERSITY OF PROLACTIN GENE IN JAPANESE QUAIL (Coturnix coturnix japonica)

Article in Press

This study was carried out to investigate the genetic diversity of prolactin gene (PRL gene) in Japanese quails using PCR-RFLP marker. One hundred and thirty nine quails were sampled for PRL loci analysis. DNA was extracted from the samples. Polymerase chain reaction (PCR) and electrophoresis was used to characterize a 24 base pair (bp) insertion/deletion in a 358 bp PCR product. Test for Hardy-Weinberg Equilibrium were...

Author(s):FRANCISCA OSEAHUMHEN EICHIE, ADEBOWALE EMMANUEL SALAKO, HENRY OSAMEDE OSAIYUWU

Phytoremediation: A general Review

Article in Press

This paper makes a general review of phytoremediation emphasising a bit on phytoextraction, in terms of factors that affect it and possible enhancements of such factors for optimization. Phytoremediation is a diverse collection of plant-based technologies that are used to clean contaminants such as heavy metals and metalloids (HMs) from environments, especially soil and water bodies. HMs are toxic to most biota, but some...

Author(s):TAOLO BONNIE TUMEDISO, B MOSEKI

Antifouling Activity of Amphiora anceps on Marine Diatoms: a Comparison with Copper Sulphate

Article in Press

The search for environment-friendly and non-toxic antifouling paint components has led to the investigation of natural products from seaweeds. The defence metabolites utilized by seaweed to deter redundant epibiosis have potential for harnessing and use in antifouling applications. A search for new non toxic antifouling paints has been tested in collected seaweed such as Amphiora anceps. The seaweed was extracted with...

Author(s):Murugan Suresh, Palanisamy Iyapparaj, Perumal Anantharaman

REVEALING EXPRESSION OF R GENES HOMOLOGUES AGAINST CLCUV IN GOSSYPIUM ARBOREUM AND G. HIRSUTUM COTTON GENOTYPES

Article in Press

Cotton leaf curl virus (CLCuV) is a major threat, limiting the fiber yield in Gossypium hirsutum. Resistance (R) genes are present in G. arboreum against this virus but these R genes are yet not known. Some resistance gene analogs (RGAs) of nucleotide binding site leucine rich repeats (NBS-LRR) have been reported in G. hirsutum. In the present study, RGAs from G. hirsutum and ESTs predicted as cell surface receptor genes...

Author(s):RAKHSHANDA MUSHTAQ, Muhammad Asif, Aftab Bashir

CANE YIELD AND JUICE VOLUME DETERMINES ETHANOL YIELD IN SWEET SORGHUM (Sorghum bicolor L. Moench)

Article in Press

Sweet sorghum (Sorghum bicolor L. Moench) contains fermentable sugars in the stem that can be converted to ethanol. The current study aimed at evaluating the performance of three sweet sorghum genotypes with five checks and contributes towards availing suitable sweet sorghum for industrial ethanol production. Field studies were carried out in Kenya at varied locations in a randomized complete block design with three...

Author(s):

Stabilization of corn oil by phenolic camellia oil cake extract during storage

Article in Press

Camellia oil cake extracts was used as a source of phenolic compounds. Isopropanol, normal hexane and diethyl ether were evaluated as different solvents to extract these phenolic compounds which examined by Folin-Ciocalteu assay. The antioxidant potential of camellia oil cake extracts was measured by ferric reducing antioxidant power assay and results showed that the antioxidant activity strongly depend on the solvent....

Author(s):FEI SONG, Hui WANG

Efficacy of botanical pesticides against Acanthoscelides obtectus (Coleoptera: Chrysomelidae): A strategy for insect pest control

Article in Press

Laboratory experiments were carried out to investigate the efficacy of botanical insecticidal materials against a major insect pest of stored beans, Acanthoscelides obtectus. The materials employed by small scale farmers to reduce the problem of bean postharvest losses caused by A. obtectus were identified and collected from Dar es Salaam and also from Kimuli and Mabira villages in Kagera region in Tanzania. The materials...

Author(s):Rugumamu Costancia Peter

Genetic improvement for green pod yield and number of pods in two segregation populations of pea (Pisum sativum L.)

Article in Press

This study was conducted at the Experimental Farm of The Faculty of Agriculture, South Valley University, Qena Governorate during three successive winter seasons of 2011/2012, 2012/2013 and 2013/2014 to improve green pod yield/plant (g) of field pea. The breeding materials used in this study were F3, F4 and F5 generations of the two field pea crosses Alaska X Little Marvel (population 1) and Alaska X Dwarf Gray sugar...

Author(s):Rashwan A., M., A

Hypolipidemic, Hypoglycemic and Anti-obesity effects of Opuntia ficus indica aqueous extract in rabbits

Article in Press

Opuntia ficus indica is used in traditional medicine for the treatment of hyperlipidemia, hypercholesterolemia, diabetes and dermal burns. The aim of this study was to analyze and compare the effects caused by the Opuntia ficus indica aqueous extract on serum and lipid parameters in white male rabbits (initial mean weight 1.95 kg). Rabbits were divided into two groups (n=6), the first group represented the control group,...

Author(s):HALMI S

COMPARATIVE STUDIES ON PRODUCTION OF BIOETHANOL FROM PEELS OF COCOYAM AND SWEET POTATO USING Aspergillus niger AND Saccharomyces cerevisiae

Article in Press

The aim of this research is to compare the potential of sweet potato and cocoyam peels (non-edible waste material) for bioethanol production using sequential monocultures of Aspergillus niger and Saccharomyces cerevisiae and co-cultures of Aspergillus niger and Saccharomyces cerevisiae. Each sample was dried and ground; and was subjected to heat pretreatment. Direct fermentation of pretreated sweet potato and cocoyam peels...

Author(s):DANIEL UDENZE and Victoria Adegunloye

USE OF MARKER ASSISTED SELECTION (MAS) FOR THE CHARACERIZATION AND INTROGRESSION OF RUST RESISTANCE GENES USING AN F2 ROBIN/KWALE POPULATION

Article in Press

Breeding for durably resistant varieties is amongst the best strategies to exterminate the threat posed by stem rust race Ug99 of wheat. This race has continued to spread and ascertain itself as a danger to global wheat production and consequently food security. The continuous swift development of new DNA marker technologies has proved invaluable in breeding for durable rust resistance to stem rust race Ug99 and its...

Author(s):BERNICE NGINA WAWERU, PETER NJOROGE NJAU, GODWIN KAMAU MACHARIA, OLIVER KIPLAGAT

A COMPARATIVE STUDY ON THE PROXIMATE AND PHYTOCHEMICAL ANALYSIS OF THE MALE AND FEMALE PARTS OF CARICA PAPAYA

Article in Press

The phytochemical analysis of the male and female plant parts (leaves, flower and bark) of Carica papaya was investigated. The analysis revealed the presence of some plant bioactive components. There was a difference in the bio-active component of the male and female leaves, both leaves revealed the presence of alkaloid, tannin, phlobotanins, saponin, flavonoid, anthraquinones, phenol and cardiac glycosides. Terpenes,...

Author(s):DEBORAH ONIFADE

Serum Total Protein concentration and Liver Enzymes Activities in albino rats model administered with ethanolic leaf extract of Ficus capensis

Article in Press

There is an increased use of the Ficus capensis plant and concerns about safety alongside efficacy have been raised. The aim of this work is to study the total protein, bilirubin and Serum Liver Enzyme activities in Albino rats administered graded concentrations of ethanol extract of F. capensis leaf. Twenty-four albino rats were shared into four groups and different concentrations of the leaf extract were administered...

Author(s):Chukwudoruo, Chieme Sunday, Osuji-Kalu-Ibe, Nnenna Chinenyenwa, Igwe, Kalu Okereke, Iheme, Callistus Izunna, Obasi, Uchechi Kingsley, Mba, Blessing Amarachi

Lactic acid bacteria fermentation of coconut milk and its effect on the nutritional, phytochemical, antibacterial and sensory properties of virgin coconut oil produced

Article in Press

Coconut oil has profound health benefits but the high content of fatty acids is a concern to many consumers, several processing methods have failed to produce oil with considerable change in fatty acid content. In this study, selected lactic acid bacteria including Lactobacillus plantarum, Lactobacillus pentosus, Leuconostoc mesenteroides and Enterococcus faecium were used to ferment coconut milk for production of virgin...

Author(s):Olateru Comfort T., Popoola Bukola M., Alagbe Gbolahan O. Ajao Omobayonle

Comparative Performance of Different Yeast Strains in the Production of Bioethanol Using Simultaneous Liquefaction Saccharification and Fermentation (SLSF).

Article in Press

The performance of a hybrid of Saccharomyces cerevisiae and Saccharomyces paradoxus (Yeast B) compared with an industrial strain of Saccharomyces cerevisiae (Yeast A) was studied. The two strains of yeasts: Yeast A obtained from the Bioresources Development Centre, National Biotechnology Development Agency Ogbomosho and Yeast B obtained from Scotch Whisky Research Laboratory in Edinburgh, Scotland (Strain 63M) were...

Author(s):Edith Lemechi Leonald-Emeka, Elizabeth Funmilayo Aransiola, Taiwo Hassan Ibrahim, Julius Olusegun Oyedele, Rose Maxwell Gidado , Andrew Chibuzor Iloh and Bamidele Ogbe Solomon,

A novel method for DNA extraction from animal blood by adding bacterial lysozyme

Article in Press

The DNA quality is one of the main conditioning factors for the individual identification. Therefore, appropriate methodologies are required for their extraction. A new DNA extraction method from sheep blood was implemented based on the hexadecyl trimethyl ammonium bromide method (CTAB 2%), 1) temperature changes, cell lysis and variation of washes (CTABM), and 2) addition of an incubation process with bacterial lysozyme...

Author(s):Fidel M. Torres-Lemus, Hilda V. Silva-Rojas, Omar Hernández-Mendo, Maria M. Crosby-Galvan.

Effects of inoculation with some native arbuscular mycorrhizal fungi on tomato (Solanum lycopersicum L.) growth

Article in Press

In Burkina Faso, tomato (Solanum lycopersicum L.) sector plays a very important socio-economic role. However, its production is confronted to many constraints, among which the soil poverty in mineral elements such as nitrogen and available phosphorus resulting in an increase in the area of land that is sown for this crop and an increase in the use of chemical inputs. In addition, chemical inputs have shown their limits...

Author(s): Hadou Haro, Kadidiata Bahadio, Kadidia B. Sanon

Yield and Stability Analysis of Promising Mung Bean (Vigna radiata L.) Genotypes in Ethiopia

Article in Press

Nine mung bean genotypes were evaluated for grain yield performance at four locations in 2016 main cropping season. The experiment was arranged in 3x3 lattice design with three replicates. Additive main effect and multiplicative interaction (AMMI) combined analysis of variance revealed that genotype, location and genotype x location interaction (GLI) significantly affected (p

Author(s):Hashim Samir Amsalu Berhanu Tumsa Kidane

Strain improvement of B. Parabrevis SR2729 for enhanced biodegradation of chlorpyrifos

Article in Press

Chlorpyrifos (CP) is of major concern for researchers and its biological degradation has been routinely practiced. Organophosphate hydrolase (OPH) responsible for the degradation of the agent was hyper-produced by the exposure of parental strain (PS) to EthBr for 70 minutes. Out of hundreds, the mutant strain SR2729-B (MS) showed 2.6 times greater zone of hydrolysis of CP, with OPH activity of 2.4 folds greater as compared...

Author(s):Samreen Rasool and Muhammad Anjum Zia

Tri-Phala Residue: A Low-Cost Substrate for Gallic Acid and Isoquercitrin Production by Aspergillus niger ATCC 16888 Under Solid-State Fermentation in Packed-bed Bioreactor

Article in Press

Tri-Phala residue (TR) was studied as a substrate for isoquercitrin and gallic acid productions by solid-state fermentation of A. niger in the packed bed bioreactor aerated at of 0.1, 0.3 and 0.5 vvm. The contents of biomass, isoquercitrin and gallic acid in the fermented TR along the height of the bioreactor were analyzed. The highest fungal growth rate was found on middle position in the SSF with 0.5 vvm aeration as...

Author(s):Teerin CHYSIRICHOTE and Pattarabhorn PAKAWEERACHAT

“PCR-based identification of Prevotella copri sp. Associated with New Onset Rheumatoid Arthritis (NORA)”

Article in Press

Rheumatoid arthritis (RA) is a prevalent systemic autoimmune disease, caused by a combination of genetic and environmental factors. Here we performed 16S sequencing on 30 samples from rheumatoid arthritis patients and controls. We identified the presence of Prevotella copri as strongly correlated with disease in new-onset untreated rheumatoid arthritis (NORA) patients. Increases in Prevotella abundance correlated with a...

Author(s):Veena K D , Prabhurajeshwar and Rashmi S B

Multi-Environment Yield Trials Analysis of Stability through AMMI and GGE Biplot Model of Cowpea (Vigna Unguiculata L.) Genotypes in Ethiopia

Article in Press

Developing high yielding cultivars with wider adaptability is the ultimate goal of any plant breeders. However, attaining this goal is complicated by genotype environment interaction. Sixteen cowpea lines including two standard checks were arranged in 4x4 triple lattice design and evaluated at seven locations for grain yield performance in 2016 main cropping season. Combined analysis of AMMI revealed that genotype,...

Author(s):Multi-environment trial, Cowpea, GEI, Broad adaptation

Qualitative Screening of Lipolytic Bacteria from Lipid–Rich Environmental Samples of Wolkite Town

Article in Press

Abstract The majority of lipolytic enzymes in industrial use are currently attracting an enormous attention because of their bio-technological applications. Lipase producing bacteria were isolated from five fat containing soils of oil mills, abattoir, garage, Sewage and cafeteria and were grown on nutrient agar. In order to identify the best lipid producing bacteria, the bacterial strain were screened in tributyrin...

Author(s):Mulugeta Belay, Getu Hailu , Ewenetu Azene, Eyuel Abasa, Meseret Neguse, Hayat Abdo, Getachew Tayachew

Molecular characterization of some bacteria with bioleaching potentials from Agbaja mines of Kogi State, Nigeria

Article in Press

Molecular studies were carried out on two bacterial species isolated from iron ore mines of Agbaja in Kogi State, Nigeria. This is with a view to exploring their bioleaching potentials. Preliminary morphological and biochemical tests shows that the two bacteria strains belong to Acidithiobacillus and Leptospirillum species respectively. X-ray diffraction analysis conducted on the raw iron ore gave concentrations of iron to...

Author(s): Ekpa, E,Onwurah I.N.E and Ezeanyika, L.U.S.

Disruption of C-terminal Domain Impairs in planta GCN2-dependent Phosphorylation of Arabidopsis eIF2α-subunit

Article in Press

Elucidation of mechanisms by which plants respond to stress is crucial for developing stress tolerant crops. Unlike plants, stress response mediated by eIF2α-kinases is well described in yeast and mammalian systems. We isolated a NaCl-tolerant mutant (JP2) with 35S enhancers upstream of AtGCN2. Under NaCl-stress, JP2 had 1.4 fold AtGCN2 transcripts than control. JP2 phenotype was however inconsistent, but prompted enquiry...

Author(s):Indieka S. A and Dominy P. J#

Evaluation of Tephrosia vogelii and Annona squamosa Extracts for their Efficacy against Insect Pests of Watermelon and their Effects on its Fatty Acid Profile

Article in Press

This experiment was carried out at Ladoke Akintola University of Technology, Teaching and Research farm during the late and early planting seasons of 2016 and 2017 to determine the efficacy of Tephrosia vogelii F. and Annona squamosal L. extracts on major insect pests of watermelon (Citrullus lanatus Thumb.) and their effects on nutritional content and fatty acid compounds of watermelon. This experiment was arranged in a...

Author(s): Fatai O. ALAO, Timothy A. ADEBAYO, Oladele A. OLANIRAN

Molecular diversity assessment using sequence related amplified polymorphism (SRAP) markers in maize

Article in Press

Sequence related amplified polymorphism (SRAP) markers were used to assess the genetic diversity and relationship among 11 inbred lines of maize (Zea mayL.) genotypes. Thirteen SRAP marker combinations were used out of which 11were found to be polymorphic with a PIC value of 0.632 discriminated all of the 11 Z. mays L. genotypes. The Jaccard’s similarity coefficients between pairs of genotypes were found to vary from 0.69...

Author(s):H. Dabral, D.C. Baskheti and R. K. Singh and V. P. Kumar

Genetic diversity of Kebericho(Echinopskebericho), an endangered, endemic Ethiopian medicinal plant as reveled byISSR molecular markers

Article in Press

Echinopske bericho is an important endangered medicinal plant that is endemic to Ethiopia. The levels of genetic diversity and patterns of population structure in E. kebericho were studied using inter-simple sequence repeat markers (ISSR). Six selected primers were used to amplify DNA samples from 70 individuals, and a total of 79 loci were detected. Our results showed that genetic diversity was high at the species level...

Author(s):Muluken Enyew, Teklehaimanot Haileselassie, Tileye Feyissa , Kassahun Tesfaye,

Cytotoxicity, genotoxicity and toxicity of plant biostimulants

Article in Press

The great expansion that in recent years has been in the use of plant biostimulants has underestimated the possibility of producing environmental risks. The objective of this study was to evaluate the cytotoxicity, genotoxicity and toxicity of three different biostimulants (widely marketed in Brazil and exported to other countries) – A, B and C - on both plant and animal indicator organisms. In A. cepa, the concentrations...

Author(s):Ana Paula Soares e Silva, Tamires de Sousa Silva, Amanda de Almeida dos Santos; Karoline Griebler Ribeiro; Márcia Maria Mendes Marques, Pedro Marcos de Almeida, Ana Paula Peron,

USE OF SIMPLE SIQUENCE REPEATS (SSRs) MOLECULAR MARKERS TO IDENTIFY COMMON BEAN (Phaseolus vulgaris L.) GENOTYPES WHICH ARE RESISTANT TO ANTHRACNOSE (Colletotrichum lindemuthianum)

Article in Press

Common bean (Phaseolus vulgaris L.), is among the most important legume crop for protein source in peoples’ diet globally and including Kenya. Anthracnose (Colletotrichum lindemuthianum) is a common disease of legumes that causes yield loss of up to 90-100%. The aim of the study was to investigate the use of SSR molecular markers to identify anthracnose resistant common bean genotypes. Forty four selected common bean...

Author(s):Gaudencia J. Kiptoo , Miriam G. Kinyua , Lexa G. Matasyoh, Oliver K. Kiplagat

ISOLATION AND CHARACTERIZATION OF A NOVEL KERATINOLYTIC BACTERIAL STRAIN FROM SOIL SAMPLES OF CHICKEN FEATHER KERATINACEOUS-WASTE DUMPING SITES OF NGAOUNDERE (CAMEROON)

Article in Press

This study aims at characterizing new chicken feather-degrading bacteria isolated from soils of keratinic waste collected from four dumping sites of Ngaoundere. Fifteen proteolytic bacteria were isolated with three (D1, D2 and F1) showing the capacity to degrade feather in Basal Salt Media. Highest degradation rate (71.11 ± 3.05 %) of feather was obtained from isolate D2 from Municipal slaughterhouse. The partial...

Author(s):SILEU DOMBOU Armelle Leslie, ADJIA ZANGUE Henriette, NSO Emmanuel JONG, NJINTANG YANOU Nicolas, TATSADJIEU NGOUNE Leopold

Biotreatment of water samples from Itakpe iron mining site, Kogi state, Nigeria using Bacillus species

Article in Press

Four soil samples and water effluents each within the mining environment were tested in this study. Twenty six bacteria were isolated using nutrient agar and acidified nutrient agar. Gram staining and endospore staining were carried out to determine the Bacillus sp. used for bioremediation. Speciation of the microorganisms using 16S rRNA sequencing showed the organisms to be; Bacillus cereus NK1, Lysinibacillus sp....

Author(s):Ogunnusi T.A., Adu, A.O.and Oyinloye, J.M.A

RAPD marker based Genetic diversity of Amaranthus population from different phytogeographic regions

Article in Press

Amaranths are most economically important group of plants, including leafy vegetables and ornamentals. Six different accessions of the same species of Amaranth were distributed from various places all over the India like Maharashtra, Gujarat and Himachal Pradesh. Correct genotype identification is important to evaluate the genetic diversity of local Amaranthus, and for efficient weed control and containing high nutritional...

Author(s):RAJIB ROYCHOWDHURY, MOBINA PARVEEN, JAGATPATI TAH

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