April 2018

ELISA, RT-PCR, semi-quantitative RT-PCR and sequencing methods for investigating an epidemic FMD virus serotype O outbreaks

This study was conducted to investigate the seroprevalence of non-structure protein for foot and mouth disease virus (FMDV) and identify FMDV serotypes. A total of 3600 serum samples (1080 buffaloes and 2520 cattle) were collected randomly from different breeds, age, and sex at El Beheira, El Dakahlia, and El Giza. Nonstructure protein of FMDV was detected in 41 of 1080 (3.8%) Buffalo and 185 of 2520 (7.3%) cattle....

Author(s): Halla E. K. El Bahgy and Mahmoud M. A. Moustafa

April 2018

In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda

In vitro somatic embryogenesis and regeneration are important techniques for crop improvement and mass propagation. This study was conducted to establish and optimize a regeneration system for agronomically important potato cultivars (‘Victoria’ and ‘Rutuku’) of Solanum tuberosum L. in Uganda. Completely randomized design experiments were set up at Bioscience East and Central Africa (BecA) Tissue...

Author(s): Arinaitwe Abel Byarugaba, Magembe Eric, Barekye Alex and Rob Skilton

April 2018

Enzymatic activity and elicitor of phytoalexins of Lippia sidoides Cham. and endophytic fungi

Secondary metabolites may play a number of roles, among them, is the activation of plant defense mechanisms, such as phytoalexins and the synthesis of antioxidative enzymes. This study aimed to test the eliciting activity of phytoalexins of Lippia sidoides Cham. and six endophytic fungi associated with it and to verify the synthesis of the enzymes, as a response to the antioxidative and antifungal system. The assays...

Author(s): Talita Pereira de Souza Ferreira, Ronice Alves Veloso, Gil Rodrigues dos Santos, Laiza Priscila dos Santos, Tatiani Pereira de Souza Ferreira, Alessandra Macedo Barros, Richard Dias Possel and Raimundo Wagner de Souza Aguiar