African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12412

Table of Content: 12 July 2012; 11(56)

July 2012

Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum)

To develop an efficient protocol for shoot regeneration of root chicory (Cichorium intybus var. sativum), some factors, including different concentrations of plant growth regulators in Murashige and Skoog (MS) medium, type of explants and genotypes were evaluated. Initiation of callusing were best achieved in MS medium supplemented with 1-naphthaleneacetic acid (NAA) (0.1 mg l-1)...

Author(s): Asad Maroufi, Mansour Karimi, Khosro Mehdikhanlou, Erik Van Bockstaele, and Marc De Loose,

July 2012

Codon optimization, constitutive expression and antimicrobial characterization of hen egg white lysozyme (HEWL) in Pichia pastoris

Fusarium oxysporum (F. oxysporum) and Verticillium dahlia (V. dahlia) causes severe cotton disease in China and other cotton-producing countries. Hen egg white lysozyme (HEWL) has antimicrobial properties. In this study, a codon-optimized HEWL gene was synthesized and cloned into the yeast expression vector, pPIC9K, under the control of the Pichia pastoris (P. pastoris)...

Author(s): Jun Li, Kevin Yueju Wang, Nan Wang, Gangqiang Li, Ning Sun and Dehu Liu

July 2012

Simple sequence repeat (SSR) and inter simple sequence repeat (ISSR) analyses of genetic diversity in tissue culture regenerated plants of cotton

Cotton is one of the main economic crop plants of Iran cultivated under continuous artificial selection and cultivation which may lead to genetic erosion and possible loss of useful genetic loci resulting in vulnerability to pests and diseases. For this reason increasing and improving the amount of genetic diversity in cotton germplasm through tissue culture is important. The present report considers genetic...

Author(s): Masoud Sheidai, Zahra Noormohammadi, Fatemeh Shojaei-Jeshvaghani, Somayeh Ghsemzadeh-Baraki, Farah Farahani, Omran Alishah

July 2012

Induction of mutations in Cichorium intybus L. by base analogue 6-aminopurine (6-AP) and their detection with random amplified polymorphic DNA (RAPD) analysis

The effect of 6-aminopurine (6-AP) on induction of mutations in Cichorium intybus L. (Asteraceae) in three consecutive generations (M1 to M3) was investigated. Three distinct mutants at 0.8% concentration were generated. These mutants were subjected to cytological analysis where 6-AP induced chromosomal aberrations such as univalents, multivalents, stickiness, laggards, bridges, etc, which reflects its...

Author(s): Zeba Khan, M. Y. K. Ansari and Honey Gupta

July 2012

Evaluation of salt tolerance in almond [Prunus dulcis (L.) Batsch] rootstocks

In this study, four interspecific Prunus rootstocks (‘HS314’, ‘HS312’, ‘HS302’ and ‘GF677’) and the Iranian almond cultivar ‘Sahand’ were subjected to four different salinity levels (1.5, 3, 6 and 9 dSm-1) to determine the effects of salt level on growth parameters and chemical compositions. The results obtained indicate that increased...

Author(s): Jalil Dejampour, Naser Aliasgarzad, Mehrshad Zeinalabedini, Mehdi Rohani niya, and Eslam Majidi Hervan

July 2012

Analysis of agromorphological diversity of southern Tunisia faba bean (Vicia faba L.) germplasm

Forty two faba bean landraces (traditional farmers’ varieties) (Vicia faba L.) from southern Tunisia, belonging to 8 oasis agro-ecosystems were assessed using morphological descriptors. The studied descriptors focused on morpho-phenological and morpho-agronomical traits. The data obtained is extracted on the basis of 35 descriptors of 42 populations from 8 localities, at a rate of 30 replications...

Author(s): Yassine  Yahia, Arbi Guetat, Walid Elfalleh, Ali Ferchichi, Hédi Yahia and Mohamed Loumerem

July 2012

Toxicity of local Malaysian Bacillus thuringiensis subspecies Kurstaki against Plutella xylostella

The toxicity effect of Bacillus thuringiensis against Plutella xylostella is well established. However, effective B. thuringiensis strain especially local isolate is not well tested. In this study local strain B. thuringiensis subspecies kurstaki, SN5 was assessed for its effectiveness against P. xylostella 3rd instar larvae. Other factors such as spore germination,...

Author(s): Patricia Jie Hung KING, Kian Huat ONG, Philip SIPEN and Nor Muhammad MAHADI

July 2012

Effect of foliar application of α-tocopherol and pyridoxine on vegetative growth, flowering, and some biochemical constituents of Calendula officinalis L. plants

A field experiment was conducted during 2010 at the Experimental Farm of Fajr, SB University of Kerman, Iran. The aim was to study the effects of foliar spray of α-tocopherol (0, 50, 100 part per million (ppm)) and pyridoxine (0, 50, 100 ppm) on vegetative growth, flowering parameters, and some chemical constituents ofCalendula officinalis L. plants. Most parameters were significantly affected by...

Author(s): Younes Soltani, Vahid Reza Saffari, Ali Akbar Maghsoudi Moud and Mitra Mehrabani

July 2012

Characterization of sorghum germplasm for various morphological and fodder yield parameters

This study was performed to evaluate and characterize 24 sorghum accessions for various morphological and fodder yield parameters. The germplasm displayed considerable variability for leaf color, midrib color, panicle shape, days to 50% flowering, leaf area, flag leaf area, plant height and green fodder yield, while differences of smaller magnitude were observed for number of leaves and tillers plant-1. Genotype Fsd...

Author(s): Durrishahwar, Muhammad Noor, Hidayat-ur-Rahman, Ihteramullah, Irfan Ahmed Shah, Farhan Ali, Syed Mehar Ali Shah and Nasir Mehmood

July 2012

Effect of substrate and cultivar on growth characteristic of strawberry in soilless culture system

The investigation was carried out for evaluation effect of substrate and cultivar on growth characteristic of strawberry in soilless culture system. Experimental treatment consisted of three strawberry cultivars (Camarosa, Mrak and Selva) and six growing media (rice hull, sycamore pruning waste, cocopeat + perlite (50:50), vermicomposts + perlite + coco peat (5:45:50), (15:40:45) and (25:35:40). Measured factors were...

Author(s): Atefe Ameri, Ali Tehranifar, Mahmoud shoor and Gholam Hossein Davarynejad

July 2012

Effect of exogenous gibberellic acid on germination, seedling growth and phosphatase activities in Lettuce under salt stress

The effect of gibberellic acid on germination and seedling growth of lettuce variety, Vista, under salinity conditions was studied. A reduction in germination percentage, roots and shoots length and fresh weight were observed under salt stress. At the same time, acid phosphatase and phytase activities in roots were reduced by NaCl. The exogenous application of gibberellin increased germination percentage and improved...

Author(s): Nawel Nasri#, Hela Mahmoudi#, Olfa Baatour, Sabah M’rah, Rym Kaddour and Mokhtar Lachâal

July 2012

Comparative activity of three inhibitors of the angiotensin converting enzyme on growth, development and ecdysteroid contents of the mediterranean flour moth, Ephestia kuehniella Zeller

Three angiotensin converting enzyme (ACE) inhibitors, enalapril, lisinopril and captopril were tested in vivo by topical application on growth, development and whole body ecdysteroids in Ephestia kuehniella Zeller (Lepidoptera:Pyralidae), an important pest in stored products worldwide. The compounds were diluted in acetone and applied topically (10 µg/2 µl) on newly molted pupae....

Author(s): Leila Kirane-Amrani and Nadia Soltani-Mazouni

July 2012

Hydrolysis of proteinaceous tannery solid waste for the production of extracellular acidic protease by Selenomonas ruminantium

The objective of this study was to produce protease from Selenomonas ruminantiumusing animal fleshing (ANFL), an untanned tannery solid waste as the sole protein source. Optimization of the minimal medium composition for the production of protease was carried out by a statistical approach using response surface methodology (RSM) which includes the variables such as NH4Cl, K2HPO4, KH2PO4and NaCl. The...

Author(s): B. Ravindran, V. Gayathri, D. Mohana Priya, S. Kanimozhi and G. Sekaran

July 2012

Effects of raw bambara nut (Voandzeia subterranea l) waste and enzyme complex on growth performance and apparent nutrient retention in broiler chickens

The effects of graded levels of raw bambara nut (Voandzeia subterranea L) waste and supplementary enzyme (Roxayzme G) on performance of broiler chicks were investigated. One hundred and twenty 14-day old unsexed commercial broiler chicks (Anak strain) were randomly divided into eight groups of 15 birds each. The groups were randomly assigned to eight energetic (9.24 to 11.68 MJ of ME/kg) and nitrogenous (21.12 to...

Author(s): A. O. Ani, O. D. Omeje and L. C. Ugwuowo

July 2012

Morphometric analysis of Dactylorhiza hatagirea (D. Don), a critically endangered orchid in cold desert Ladakh region of India

The morphometric study was conducted during 2009 to 2010. About 28 morphological characters were measured under 13 natural populations of Dactylorhiza hatagirea.Geographic variation in morphology reflects phenotypic responses to environmental gradients and evolutionary history of populations and species. At points, beside its broad geographic range (Nubra, Suru and Indus valley) characterization...

Author(s): Ashish R. Warghat, Prabodh K. Bajpai, Hemant Sood, Om P. Chaurasia, and Ravi B. Srivastava