African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 14 January, 2015; 14(2)

January 2015

Simple sequence repeat (SSR) markers are effective for identifying pear cultivars and selections

The present study characterized and identified pear cultivars growing in the southern region of Minas Gerais State, Brazil, using microsatellite markers. Nineteen (19) pear cultivars were collected from two sites of Southern Minas Gerais State: Ribeirão Vermelho and Lavras. DNA was extracted from newly formed leaves and amplified using 21 simple sequence repeat (SSR) markers (NH001c, NH002b, NH005b, NH007b,...

Author(s): Juliana Stracieri⊃, Hélida Mara Magalhães⊃, Luciane Vilela Resende⊃ and Luis Carlos Cirilo Carvalho⊃

January 2015

Assessment of genetic relationships within Bouea (Anacardiaceae) accessions in Peninsular Malaysia using inter simple sequence repeats (ISSR) markers

Malaysia is a genetically diverse hotspot for wild relatives of Mangifera and other Anacardiaceae members. The genus Bouea is interesting in that it has small sweet-sour edible fruits. This genus consists of two species, namely Kundang (Bouea macrophylla) and Remia (B. oppositifolia) which are of economic value. In this study, we highlighted the molecular evidences using inter simple sequence repeats (ISSR) markers to...

Author(s): Mohd Norfaizal Ghazalli, Muhamad Fahmi Yunus and Abdul Latiff Mohammad

January 2015

Genetic diversity of Tunisian melon (Cucumis melo. L) landraces and their relationships with introduced varieties as assessed by simple-sequence repeat (SSR) markers

The genetic diversity and the relationships among Tunisian melon landraces and introduced varieties belonging to different varietal groups were estimated using six simple-sequence repeat (SSR) markers. All loci were polymorphic and provided a total of 56 alleles, with an average of 9.33 alleles per locus. The allelic frequencies differed according to accessions, and particular alleles were found within several...

Author(s): Rym TRIMECH, Makram AFIF and Mohamed BOUSSAID

January 2015

Identification of genes that have undergone adaptive evolution in cassava (Manihot esculenta) and that may confer resistance to cassava brown streak disease

Cassava (Manihot esculenta) is a vital food security crop and staple in Africa, yet cassava brown streak disease (CBSD) and cassava mosaic disease result in substantial yield losses. The aim of this study was to identify genes that have undergone positive selection during adaptive evolution, from CBSD resistant, tolerant and susceptible M. esculenta varieties and inter-specific hybrids, as well as a wild cassava...

Author(s): M. Ferguson, T. M. Koga, D. A. Johnson, K. A. Koga, G. N. Hirsch, L. A. Becerra Lopez-Lavalle, and W. Messier

January 2015

Micropropagation of Phytolacca dodecandra L'Herit (Endod var. E-44)

Phytolacca dodecandra L’Heit (Endod var. E-44) is a medicinal plant of high saponin content used as a snail control agent in schitosomiasis. The aim of this study was to develop a micrpropagation protocol for P. dodecandra. Nodal and shoot tip explants were sterilized and cultured on MS medium supplemented with different types of concentrations and combinations of auxin and cytokinin for shoot induction,...

Author(s): Jiregna Daksa, Balcha Abera and Tewodros Taddese

January 2015

Forage crops as substrate for animal feed and ethanol production in Thailand

Five forage crops, namely ruzi (Brachiaria ruziziensis), purple guinea (Panicum maximum), atratum (Paspalum atratum), plicatulum (Paspalum plicatulum), and rhodes grass (Chloris gayana), were experimented for their possibility of ethanol and animal feed utilization. All tested forage crops were harvested 45 and 75 days after being planted. The results indicate the effect of harvesting time on their composition,...

Author(s): Pilanee Vaithanomsat, Phanawan Thongsuntie, Phatthara Khomkamon and Darunee Srichana

January 2015

Evaluation of extracts of Coleus species for antibacterial activity

Antibacterial activity of 50%-methanol, water and absolute methanol extracts of Coleus species (whole plant) was evaluated using agar, and macro-broth dilution methods. The test microorganisms were strains of Staphylococcus aureus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa. The yields achieved with extracts were 24% for absolute methanol, 22% for the water and 39% for the 50% methanol extracts,...

Author(s): Jacqueline Ebob Tarh, Josephine Ifeanyinwa Okafor and Christian Ukwuoma Iroegbu

January 2015

Milk clotting and proteolytic activity of enzyme preparation from Pediococcus acidilactici SH for dairy products

Some microorganisms have the ability to produce enzymes that could clot milk and used as a substitute for calf rennet. Strains of lactic acid bacteria (LAB) could produce proteolytic enzymes that may have the potential to be used as a source of milk clotting enzyme (MCE). In the present study, LAB isolated from shrimp paste identified as Pediococcus acidilactici SH, was evaluated for milk clotting activity (MCA),...

Author(s): Mohamed Muftah Imdakim, Zaiton Hassan, Mohamed Mustafa Aween, brahim Mustafa Elshaafi and Belal Jamal Muhialdin

January 2015

Chemical and physicochemical characterization of the seed oil from ‘Gefner’ atemoya

Atemoya is a hybrid fruit derived from the crossing of sugar-apple (Annona squamosa L.), with cherimoya (Annona cherimola Mill.). The objective of this study was to compare two extraction methodologies for the seed oil from ‘Gefner’ atemoya, for yield, chemical and physicochemical characteristics, in order to use the oil as biofuel. The physical extraction was shown to be more economically viable than the...

Author(s): Luciana Soares da Cruz, Angelita Duarte Corrêa, Valquíria Alves Bastos, Pedro Castro Neto and Luciana de Matos Alves Pinto

January 2015

Physicochemical and sensory profile of yogurt added with passion fruit peel flour

Yogurt is a type of fermented milk with high nutritional value. The addition of passion fruit peel flour to yogurt may help the retention of water due to the presence of starch, in addition to being a source of nutrients and fiber. Thus, the aim of this study was to evaluate the physicochemical parameters, color and sensory profile of yogurt added with passion fruit peel flour. Four yogurt formulations with...

Author(s): Vieira, N. F., Silva, M. A. P., Martins, Y. A. A., Souza, D. G., Lima, M. S., Plácido, G. R. and Caliari, M.

January 2015

Histologic and ultrastructural observations on the thyroid gland of the White Fulani (Zebu) cattle in Northern Nigeria

The histology and ultrastructure of thyroid gland of White Fulani (Zebu) cattle were examined during the postnatal developmental period to show if variations in morphology exist with age and in relation to tropical climate. Eight prepubertal and ten pubertal cattle of abattoir origin were used to carry out this study. Histologically, the thyroid consisted of well-developed capsule, interlobular connective tissue,...

Author(s): Casmir Onwuaso Igbokwe and Daniel Nwagbo Ezeasor