African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12267

Table of Content: 14 September, 2016; 15(37)

September 2016

Perennial soybean seeds coated with high doses of boron and zinc

The objective of this work was to study combinations of high doses of boron (B) and zinc (Zn) in the recoating of perennial soybean seeds, in order to provide these nutrients to the future plants. The physical, physiological and nutritional characteristics of the coated seeds and initial development of plants in a greenhouse were evaluated. Tests carried out in the laboratory were conducted in a completely randomized...

Author(s): Amanda Justino Acha, Henrique Duarte Vieira and Marta Simone Mendonça Freitas

September 2016

Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

Jatropha curcas L. oil has been shown to be suitable for the production of biodiesel. However, this species has not been domesticated yet. Genetic breeding through conventional methods is time consuming and costly, hence, genetic transformation could contribute positively to the improvement of interesting traits. Although in vitro regeneration and stable genetic transformation has been pursued for several years,...

Author(s): Mariana Crotti Franco, Kleber Alves Gomes, Marcel Mamede de Carvalho Filho, Ricardo Harakava, Nicolas Carels, Walter José Siqueira, Rodrigo Rocha Latado and Daniela de Argollo Marques

September 2016

Germination, growth and physiological responses of Senegalia senegal (L.) Britton, Vachellia seyal (Delile) P. Hurter and Prosopis juliflora (Swartz) DC to salinity stress in greenhouse conditions

Salinity is among the most widespread environmental threats to global plant production, especially in arid and semi-arid climates. Thus, the selection of salt tolerant species is necessary for sustainable plant productivity. The purpose of this study was to measure and understand the salt tolerance of three multipurpose trees used in reforestation programs in many Sahelian countries (Senegalia senegal, Syn. Acacia...

Author(s): Dioumacor Fall,, Niokhor Bakhoum, Fatoumata Fall,, Fatou Diouf, Mamadou O. Ly, Mayécor Diouf, Djamel Gully, Valérie Hocher and Diégane Diouf,

September 2016

Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Ă— Elaeis oleifera)

Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were...

Author(s): Bonetti, K. A. P., Nesi, J., Quisen, R. C. and Quoirin, M.

September 2016

Genetic diversity of rice (Oryza sativa) germplasm from six countries using simple sequence repeats markers

This experiment was carried out to determine the genotypic variation among rice (Oryza sativa) accessions using simple sequence repeats (SSR) markers. In the present study, a total of 12 SSR markers were used across 87 rice accessions from six countries. NTSYS-pc and PowerMarker software were used for data analysis. Six primers out of these 12 primers showed DNA amplification and polymorphism among the 87 rice...

Author(s): Ibrahim Soe, Richard Akromah, Daniel Nyadanu, Maxwell Darko Asante and Alex Tamu

September 2016

The rolling circle amplification and next generation sequencing approaches reveal genome wide diversity of Kenyan cassava mosaic geminivirus

Rolling circle amplification is a simple approach of enriching populations of single-stranded DNA plant begomovirus genomes (genus, Begomovirus; family, Geminiviridae). This is an innovative approach that utilizes the robustness of the bacteriophage phi29 DNA polymerase used in circle amplification, together with deep sequencing using Illumina Miseq and bioinformatics to assess population diversity of begomoviruses in...

Author(s): T. M. Kathurima, , E. M. Ateka, A. B. Nyende and T. A. Holton

September 2016

Influence of recirculation rate on the performance of a combined anaerobic-aerobic reactor applied to the removal of carbon and nitrogen from poultry slaughterhouse wastewater

The objective of this study was to evaluate a combined anaerobic-aerobic upflow fixed-bed reactor with liquid phase recirculation for the removal of nitrogen and organic matter from poultry slaughterhouse wastewater. The reactor performance was evaluated with a hydraulic retention time (HRT) of 11 h and three different recirculation rates (R=0.5; 1 and 2). The highest nitrogen removal efficiency value was obtained with...

Author(s): Carla L. Lopes, Adriana N. de Lima Model, Jackeline T. Gotardo, Fernando H. Passig, Eloy L. de Mello, Benedito M. Gomes and Simone D. Gomes

September 2016

Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)

Trichrome stain (such as Van Geison) is usually used in histopathology laboratory for demonstration of collagenic fibers. Lack of selectivity and tendency of stain to fade makes van Gieson not ideal for collagen demonstration. This study was aimed to compare between Giemsa's and van Gieson's stains in collagen fibers demonstration. Twenty biopsies were obtained from rabbit’s skin after anesthesia by...

Author(s): Ziyad Mudasir, Salma Adil and Ahmed Ibn Edriss