July 2015
Molecular cloning and expression of a novel gene related to legume lectin from Salvia miltiorrhiza Bunge
Lectins have been well studied and proved to play important roles in plant defense but information of legume lectins from non-legume plants has been rarely reported. A new legume lectin gene, designated as SmL1, was cloned from Salvia miltiorrhiza Bunge, a famous traditional Chinese medicinal plant. The cDNA of SmL1 was 919 bp in length and contained an 822 bp open reading frame (ORF) encoding a putative lectin...
July 2015
Molecular screening for erythromycin resistance genes in Streptococcus pyogenes isolated from Iraqi patients with tonsilo-pharyngites
Streptococcus pyogenes is the leading cause of uncomplicated bacterial pharyngitis and tonsillitis commonly referred to as strep throat. Erythromycin is administered for patients allergy to penicillin. In this study, 125 throat swab samples were collected from children between 2-12 years old with tonsillo-pharyngitis attended to at the AL-Imammain AL-Kadhimain Medical City-Baghdad-Iraq and Pediatric...
July 2015
Antifungal, acute toxicity and mutagenicity activity of extracts from Datura stramonium, Jacquinia macrocarpa and Krameria erecta on Fusarium verticillioides
The effect of Baccharis glutinosa, Jacquinia macrocarpa, and Krameria erecta extracts was investigated on the growth and the spore germination of Fusarium verticillioides (ATCC 52539). Brine shrimp (Artemia salina) was used to evaluate the potential acute toxicity of the fractions obtained from plant extracts. The butanol fraction of J. macrocarpa totally inhibited the radial growth for 144 h and up to 95% after 168 h....
July 2015
Connecting DNA origami structures using the biotin-streptavidin specific binding
This work made use of the strong interaction between biotin and streptavidin to connect designed DNA origami structures. The caDNAno software was used to design a 6 layer 3D origami cross-like structure. Selected DNA strands at the engineered attachment sites on the DNA origami structure were biotinylated. After folding of the origami structures, the biotinylated strands stick out of the attachment sites. Purified...
July 2015
Reproductive performance of dairy cows affected by endometritis, pododermatitis and mastitis
The effects of endometritis, pododermatitis and clinical mastitis on the conception rate and calving-conception interval of multiparous and primiparous cows after fixed-time artificial insemination (FTAI) were evaluated. Clinical endometritis was diagnosed by ultrasonography 20-40 days postpartum upon observation of fluid in the uterine lumen. Cows with clinical endometritis were treated intramuscularly with 2 mg/kg...
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