October 2013
Associated technologies ensures complete loop mediated isothermal amplification platform for pathogen diagnosis
Loop Mediated Isothermal Amplification (LAMP) assay could be a useful adjunct diagnostic assay along with the conventional methods that would preclude the requirement of continuous maintenance of pure cultures. Moreover, LAMP assay is simple, rapid, specific and sensitive for the detection of pathogens. Having developed and validated LAMP method for the detection of an isolated...
October 2013
Random amplified polymorphic DNA (RAPD) markers reveal genetic diversity in bael (Aegle marmelos Correa) genotypes of Andaman Islands, India
The present study evaluated genetic variability of superior bael genotypes collected from different parts of Andaman Islands, India using fruit characters and random amplified polymorphic DNA (RAPD) markers. Genomic DNA extracted from leaf material using cetyl trimethyl ammonium bromide (CTAB) method was subjected to polymerase chain reaction (PCR) with 12 polymorphic primers. A total of 476 polymorphic loci...
October 2013
Genetics of seed coat color in sesame (Sesamum indicum L.)
Seed coat colour of sesame is commercially an important trait. Developing white seeded varieties with long lasting luster has received momentous attention in most of the major sesame producing countries including India. The present investigation centered on the genetic control of seed coat colour in sesame. No genetic nomenclature is available in sesame to describe seed coat colour. This is the first attempt to...
October 2013
Molecular and genetic study of wheat rusts
Puccinia triticina, Puccinia graminis and Puccinia striiformis cause leaf, stem and yellow rust, respectively. Wheat rusts can cause losses as high as 70%. The rusts ability to evolve fungicide resistance has resulted in the use of resistant cultivars as the primary method of control. Breeding resistant cultivars is a long process and requires an accurate picture of the current and future pathogen...
October 2013
Isolation of NBS-LRR class resistant gene (I2 gene) from tomato cultivar Heamsona
Three races of Fusarium oxysporum f. sp. lycopersici race 1, 2 and 3 are identified depending on the avirulence protein or effector protein secreted by fungal pathogen during the host colonization in tomato. These effector proteins are recognized by the host innate immune system based on R gene expressions that are I1, I2 and I3 in tomato for each races. Amongst the three, I2 protein has been cloned...
October 2013
Nutritional composition, vitamins, minerals and toxic heavy metals analysis of Trianthema portulacastrum L., a wild edible plant from Peshawar, Khyber Pakhtunkhwa, Pakistan
This study aimed at analyzing the nutritional potential of a wild edible plant, Trianthema portulacastrum L. widely used in Peshawar, Khyber Pakhtunkhwa, Pakistan. The nutritional composition, vitamins, minerals and heavy elements were analyzed following the standard methods of Association of Official Analytical Chemists (AOAC). The nutritional assessment included determination of moisture, ash, lipid, fiber,...
October 2013
Can Bt maize change the spatial distribution of predator Cycloneda sanguinea (L.) (Coleoptera: Coccinelidae)?
Cultivation of Bt crops is an important tactic in integrated pest management. The effect of Bt maize on arthropod predators needs to be investigated because of the important role of these natural enemies in the absence of target pests. The objective of the present study was to generate information on the distribution model ofCycloneda sanguinea (L.) (Coleoptera: Coccinelidae) in Bt and non-Bt maize. A sampling...
October 2013
Bioremediation of soil contaminated with spent and fresh cutting fluids by Pleurotus pulmonarius (Fries) Quelet
Contamination of soil with industrial cutting fluids containing heavy metals and petroleum hydrocarbons has detrimental effects on ecosystems. As such contaminants constitute risk to human health; they can enter the food chain through agricultural products or contaminated drinking water. This growing concern about contamination with cutting fluids makes remediation process imperative. White rot fungus, Pleurotus...
October 2013
Moisture dependent physico-mechanical properties of Iranian okra (Ablemoschus esculentus L.) seed
Physical and mechanical properties of mature okra (Hibiscus esculentus L.) seeds from Ahvaz in Iran were evaluated. The physical and mechanical properties were evaluated at four moisture content levels of 7.1, 10, 15 and 20% dry basis (d.b). In this moisture range, seed length, width, thickness, geometric diameter, mass of 1000 seeds increased from 5.096 to 5.677 mm, 4.476 to 4.878 mm, 4.239 to 4.608 mm, 4.585...
October 2013
Effect of soil moisture management on the quality of wax apple
Wax apple (Syzygium samarngense Merr.et Perry) was one of the economically planted fruits in Taiwan. This research was conducted to evaluate the effects of different soil moisture management on increasing wax apple quality. It was preceded at two different soil properties (shallow soil and alluvial soil) in Pingtung, Taiwan. Three different treatments were used at the two areas in the fruit setting stage: (1)...
October 2013
Isolation and identification of a Staphylococcus warneri strain with anti-mycobacterial activity
Tuberculosis is the principal cause of death from infection in the world. The resurgence of tuberculosis and the increase in mycobacterial infections, as well as multidrug-resistance of mycobacteria to available antibiotics, has incentivized research on new antimycobacterial agents. Therefore, research based on water and soil samples from the Moroccan biotopes, has led to the isolation of a bacterial strain...
October 2013
Effect of partially purified fumonisins on cellular immune response in experimental murine paracoccidioidomycosis
Fumonisins are mycotoxins produced mainly by Fusarium verticillioides, which can modulate the immune response. Paracoccidioidomycosis (PCM), caused by the fungus Paracoccodioides brasiliensis (Pb), is one of the most important systemic mycoses in Latin America. The aim of this study was to evaluate the effect of the partially purified fumonisins on cellular immune response in mice infected with Pb. Four...
October 2013
Pharmacognostic and phytochemical evaluation of the Solanun sisymbriifolium leaf
Solanum sisymbriifolium Lam. (Solanaceae) is an important medicinal herb in Ayurvedic medicine. In the present investigation, the detailed pharmacognostic study of S. sisymbriifolium leaf was carried out to lay down the standards which could be useful in future experimental studies. The study included macroscopy, microscopy, preliminary phytochemical screening and physicochemical evaluation. These...
October 2013
Genotypic profiling of coding region of leptin gene and their association studies on reproductive and milk production traits in Sahiwal and Frieswal cattle of India
Leptin gene has its role in appetite, metabolism, growth and milk production in cattle. Single nucleotide polymorphisms (SNPs) in leptin gene in different cattle breeds have been reported and subsequently associated with their production performance. The objective of this study was to evaluate the association of genetic differences in the bovine leptin gene with milk production, reproduction, milk constituents in...
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