African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 17 December 2014; 13(51)

December 2014

Diversity analysis of Rhizoctonia solani causing sheath blight of rice in India

Sheath blight (Rhizoctonia solani) causes severe loss in the production of rice in India. Twenty five (25) isolates were collected from different parts of India. Morphological and cultural characters were investigated, on the basis of colony colour, growth pattern, hyphal width, colony growth, formation of sclerotia, size of sclerotia, diameter and number of sclerotia. Pathogenic variability was studied on highly...

Author(s): Mehi Lal, Vivek Singh, Janki Kandhari, Pratibha Sharma, Vinay Kumar and Shiv Murti

December 2014

Characterization of pathogen responsible for infection in bhindi plant by using phytoplasma specific universal primers

Infection in bhindi plant (Abelmoschus esculentus, Family-Malvaceae) is very common in Gorakhpur district of Eastern U.P. region of India. To detect the pathogen, genomic DNA was isolated from the infected bhindi plant sample and amplified through polymerase chain reaction (PCR) by using P1 forward primer and Tint reverse primer, which were phytoplasma specific universal primers. Obtained PCR amplicons were cloned...

Author(s): Richa Tiwari, Sudhanshu Tiwari and P. P. Upadhyaya

December 2014

Effect of elicitation on picrotin and picrotoxinin production from in vitro products of Picrorhiza kurrooa

Picrorhiza kurrooa Royel ex. Benth. is an important medicinal plant of Himalayan region and a good source of iridoid glycosides. Picrotin and picrotoxinin are compounds produced by P. kurrooa which are widely used in treatment of hepatic diseases. Elicitation is one of the best effective methods which enhance secondary metabolite production in plants.  In the present study effect of elicitors for example, Methyl...

Author(s): Janhvi Mishra Rawat, Balwant Rawat, and Susmita Mishra

December 2014

Genetic characterisation of avocado (Persea americana Mill.) in two regions of Ghana

Avocado (Persea americana Mill.) is a nutritious and economic tree crop with cultivations scattered all over Ghana. Although, there have been some genetic diversity research on this crop in some parts of the world, rather limited research has been done on avocado in Ghana. A study was therefore conducted to assess its genetic diversity in the Ashanti and Central Regions of Ghana. Microsatellites analyses revealed 115...

Author(s): Janice Dwomoh Abraham, and Jemmy F. Takrama

December 2014

Fusarium branch blight on highbush blueberry in Argentina

In Argentina, highbush blueberry (Vaccinium corymbosum) is grown in Tucumán, Entre Ríos and Buenos Aires provinces. In the 2010 to 2011 cropping season, a new disease with 10% incidence was observed on the foliage of “Emerald” plants in Concordia, Entre Rios. Symptoms included acropetal blight and premature branch death. Leaves remained attached and showed tan to brown discoloration. A fungal...

Author(s): Eduardo Roberto Wright, Marta Carolina Rivera, Eduardo Raúl Campanella, Omar Marcelo Farinon, Marcelo Facundo Berretta and Beatriz Alida Pérez

December 2014

Comparative study on early detection of sugarcane smut (Sporisorium scitamineum) by polymerase chain reaction (PCR) and microscopy

Sugarcane smut caused by Sporisorium scitamineum has the potential to result in substantial tonnage loss and significant reduction in sucrose recovery. As early and precise diagnosis is an integral component in the successful management of sugarcane smut, the present study was undertaken to accurately determine the presence of pathogen employing PCR-based methods supplemented with microscopy. Healthy sets of sugarcane...

Author(s): M. Kavitha, A. Ramesh Sundar, P. Padmanaban, R. Viswanathan and P. Malathi

December 2014

Variants of NAT2 polymorphisms: Intra and inter-ethnic differences

N-Acetyltransferase 2 (NAT2) gene is known for its polymorphism. The genetic variations leads to the change in the N-acetylation activity and these differences in the acetylation activity leads to the classification of the population into various groups such as rapid, intermediate and slow acetylators. In the present study, we identified different mutations and alleles by sequencing a stretch of 927 bp which covered...

Author(s): Neelam Chauhan, and Harish Padh,