September 2006
The potential of mitochondrial DNA markers and polymerase chain reaction-restriction fragment length polymorphism for domestic and wild species identification
Poaching is increasingly presenting challenge to conservational authorities in Africa.xe "Hofer et al., 1996" Accurate and reliable methods for the identification of poached wildlife meat when morphological features are missing, has been lacking in Africa. We describe a molecular based approach that has a potential of serving as a tool for game and domestic meat identification in Africa. A...
September 2006
Efficient production of transgenic soybean (Glycine max [L] Merrill) plants mediated via whisker-supersonic (WSS) method
The present study was designed to evaluate the transformation efficiency and proof the capability of whisker supersonic (WSS) method as an alternative option for soybean (Glycine max [L] Merrill) transformation. We compared soybean transformation efficiency obtained by WSS-mediated with that of particle bombardment transformation by carrying out molecular analysis of the T0 plants in two independent...
September 2006
Identification of Bacitracin Produced by Local Isolate of Bacillus licheniformis
Bacillus licheniformis was isolated from soil of different house gardens. Diagnosis was performed according to Gram stain, motility, shape forming, aerobic condition and other tests. Bacitracin was primary identified after its activity was tested against some species of Gram positive and Gram negative bacteria. Identification was completed by using thin layer chromatographic technique. Key...
September 2006
Evaluation of antibacterial activity and phytochemical analysis of root extracts of Boscia angustifolia
The aqueous and organic solvents extracts of Boscia angustifolia were screened for antibacterial and phytochemical properties. Alkaloids and saponins were detected in aqueous and chloroform extracts. These extract fractions were significantly (p<0.05) active against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Streptococcus pneumoniae but did not show activity...
September 2006
Effect of Taungya on regeneration of endemic forest tree species in Nigeria: Edo State Nigeria as a case study
Taungya as an agroforestry system in regenerating endemic forest tree species is not successful in Edo State. This was revealed in the current study. Significant differences in natural regeneration exist in the zones studied. The failure of the Taungya system arose from abandonment of the sites after devegetation, non availability of planting stock which is due to poor funding. The area that was kept under continuous...
September 2006
Influence of pharmaceutical effluent on some soil chemical properties and early growth of maize (Zea mays L)
A greenhouse experiment was conducted at the University of Benin, Benin City, Nigeria to determine the effect of pharmaceutical effluent on some soil chemical properties and early growth rate of maize (Zea mays L) in an experiment laid out in a completely randomized design with three replicates. Five rates of the effluent- 0, 25,000, 50,000, 75,000 and 100,000 l/ha were used. Results of the effluent analysis...
September 2009
Comparative studies of drying methods on the seed quality of interspecific NERICA rice varieties (Oryza glaberrima x Oryza sativa) and their parents
Seed moisture content (MC) is a key component that determines storability of germplasm conserved in seed genebanks. The purpose of this research was to compare the efficiency of sun-, shade-, silica gel- and conventional room drying in terms of rice seed MC and viability using seeds of two interspecific progenies(NERICA 1 and NERICA 3) and their parents Oryza glaberrima and O. sativavarieties...
September 2006
Bioautography indicates the multiplicity of antifungal compounds from twenty-four southern African Combretum species (Combretaceae)
Dried ground leaves of 24 Combretum spp were extracted with hexane, dichloromethane, acetone and methanol and analysed by bioautography to determine the number of antifungal compounds against five animal fungal pathogens (Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus,Microsporum canis and Sporothrix schenckii). There was some similarity in the chemical composition...
September 2006
Is Vernonia amygdalina hepatotoxic or hepatoprotective? Response from biochemical and toxicity studies in rats
The effects of various concentrations of aqueous extract of Vernonia amygdalinaleaves on some biochemical indices of liver function were investigated in albino Wistar rats. Acute toxicity tests of the extract gave an LD50 of 500 mg/kg. Phytochemical analysis of the plant material showed that anthracene glycosides, steroids, flavonoids, proteins, carbohydrates, reducing sugars, saponins and...
September 2006
Chemical composition of volatile constituents from the leaves of Aloe ferox
Volatile compounds oils play a significant role in perfumery, cosmetic, medicinal and pharmaceutical ndustries. In our protracted research for new useful essential oils, a volatile oil from Aloe ferox was obtained by olonged hydrodistillation. This volatile oil was subjected to GC/MS analysis to identify the major constituents of the oil. The major constituents identified were as follows: 3, 6 octatriene (23.86%),...
September 2006
Multiplicity of infections and level of recrudescence in Plasmodium falciparum malaria in Mlimba, Tanzania
Polymorphism and antigenic variation are important biological survival strategies of malaria parasites determining the episode, outcome and implications of treatment interventions. In P. falciparum, polymorphic antigens are associated with the asexual blood-stage; merozoite surface protein 2 (MSP2). The MSP2 genes have been invaluable in post-treatment discrimination of parasite resurgence from new infection,...
September 2006
Antimicrobial activities of Vernonia tenoreana
The antimicrobial activity of Vernonia tenoreana was investigated. Crude extracts and solvent fractions of the leaf and bark of V. tenoreana were obtained using methanol and distilled water (2:3, v:v), ethylacetate, chloroform, and n-hexane as solvents of extraction. The crude and solvent fractions showed broad spectrum activities against Staphylococcus aureus, Streptococcus faecalis,...
September 2006
Ethanolic extracts of Sophora moorcroftiana seeds induce apoptosis of human stomach cancer cell line SGC-7901 in vitro
The seeds of Sophora moorcroftiana are used in Chinese traditional medicine for the treatment of verminosis, infectious diseases, and anti-inflammation. To investigate the antitumor and induction of apoptosis activity of Sophora moorcroftiana seeds, the ethanolic extracts from the seeds was prepared and added into the culture of human stomach cancer cell line SGC-7901 in vitro. The proliferation and...
September 2006
Prevalence of hypocalcaemia in patients attending the Olabisi Onabanjo University Teaching Hospital, Sagamu, South Western Nigeria
Nine hundred and two (902) patients were randomly selected for the study from the patients that reported in the Clinical Biochemistry Laboratory of Olabisi Onabanjo University Teaching Hospital, Nigeria. The period of study was two years. The prevalence of hypocalcaemia was 11.53% (serum calcium level = 8.36±0.40 mg/100 ml), and reference range for serum calcium of our laboratory = 9 - 11 mg/100 ml (2.25 - 2.75...
September 2006
Treatment of textile sludge using anaerobic technology
Composite samples of sludge obtained from a textile factory were characterized for their pollution characteristics using some parameters of interest; pH, solids concentration, oxygen demand, nitrogen, phosphorus, total bacteria counts etc. The analysis revealed that the sludge has high pollution potentials and therefore needed treatment before disposal to the environment. The ratio of chemical oxygen demand, (COD) to...
September 2006
Production of peptone from soya beans (Glycine max L merr) and African locust beans (Parkia biglobosa)
Peptone was produced from soya beans and African locust beans. The produced peptones were evaluated as component of microbiological media for the growth of some bacteria and compared with some commercial peptones. Some of the tested bacteria are Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. The soya beans and African locust beans peptones supported the growth of...
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