African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 18 September 2013; 12(38)

September 2013

Genetic diversity and demographic evolution of baobab (Adansonia digitata L., Bombacoideae, Malvaceae) populations in Senegalese Sahelian areas

This study evaluated the spatial genetic structure of baobab (Adansonia digitata L.) populations from three agroecological sites located in sahelian zone of Senegal usingITS1, 5.8S rDNA and ITS2 gene sequences. To determine the extent of isolation, gene sequences were analyzed between and among three sahelian baobab populations. At least 25 haplotypes of baobab (A. digitata L.) were...

Author(s): Amadou Lamine NDOYE, Toffène DIOME, Mame Codou GUEYE, Mbacké SEMBENE, and Mame Ourèye SY

September 2013

Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.) cultivar K. badshah

Kufri badshah is one of the important medium maturing, blight resistant potato varieties with round to oblong tubers; it has yellowish skin, shallow eye and white pulp. This variety is popular among farmers. The study on development of tissue culture protocol was carried out using sprout as an explant for initiation of culture in MS media supplemented with eleven different combinations of growth...

Author(s): Gami R. A., Parmar S. K., Patel P. T., Tank C. J., Chauhan R. M., Bhadauria H.S. and Solanki S.D.

September 2013

Influence of vesicular arbuscular mycorrhiza (VAM) and phosphate solubilizing bacteria (PSB) on growth and biochemical constituents of Marsdenia volubilis

A field experiment was carried out to find out the effect of biofertilizers, vesicular arbuscular mycorrhiza (VAM), and phosphate solubilising bacteria (PSB) individually and in combination on growth and physiological attributing properties of Marsdenia volubilis plant under nursery conditions. The plant seedlings were harvested at various intervals: 30, 60 and 120 days after transplantation. The inoculation...

Author(s): A. Sandhya, T. Vijaya, A. Sridevi and G.Narasimha

September 2013

Nutrients, phytochemicals, fungal flora and aflatoxin in fresh and salted Vernonia amygdalina leaves

In this study, the effect of salting on the pH, phytochemicals, fungal flora and nutrient composition of Vernonia amygdalina leaves was investigated. There was a decrease in pH from 5.88 for the fresh, to 5.80, 5.73, 5.24, and 5.02 for the light brined, light salted, heavy salted and light brine + vinegar treated leaves, respectively. Leaves treated with water alone had a pH of 6.63. Compared with the...

Author(s): Fred O. J. OBOH, Anita ALIU, Monday I. IDEMUDIA and Derek AHAMIOJE

September 2013

Use of Lactococci isolated from Moroccan traditional dairy product: Development of a new starter culture

Ninety (90) strains were isolated from Moroccan traditional dairy product and identified using biochemical and molecular tests. Among the 20 identified lactic acid cocci, two strains were selected for their important acidifying activity: Lactococcus lactis subsp. lactis (AML8) and Lactococcus lactis subsp. cremoris (BML2). Both revealed remarkable acidifying activity, especially when...

Author(s): Najat Bekkali, Amina El Amraoui, Aayah Hammoumi, Véréna Poinsot and Rajae Belkhou

September 2013

Effects of natural plant tenderizers on proteolysis and texture of dry sausages produced with wild boar meat addition

This study was conducted to develop a method for improving tenderness and overall qualities of tough wild boar meat used to dry sausage production with direct addition of raw pineapple (Ananas comosus), mango (Mangifera indica), kiwifruit - fuzzy kiwi (Actinidia deliciosa), or ginger (Zingiber officinale roscoe - ginger rhizome) juices contained a plant proteolytic enzyme. Dry-sausages were subjected to various...

Author(s): J. Å»ochowska-Kujawska, K. Lachowicz, M. Sobczak, A. NÄ™dzarek and A. Tórz  

September 2013

Extracellular β-D-fructofuranosidase from Aspergillus parasiticus: Optimization of the production under submerged fermentation and biochemical characterization

The β-D-fructofuranosidases are enzymes with biotechnological potential that can be used in different industrial sectors as food and beverage. In this context, microorganisms are important producers of these biomolecules, especially filamentous fungi. The production of extracellular β-D-fructofuranosidase fromAspergillus parasiticus using sugarcane bagasse as a carbon...

Author(s): André Luis Lucca, João Atílio Jorge and Luis Henrique Souza Guimarães

September 2013

Thermal and pH stabilities of partially purified polyphenol oxidase extracted from Solanum melongenas and Musa sapientum fruits

Enzyme activity depends largely on environmental conditions such as temperature and pH. The stability of polyphenol oxidase (PPO) extracted from Solanum melongenas and Musa sapientum fruits pre-incubated in varying thermal and pH conditions were carried out. Enzyme activity was measured by spectrophotometric methods. The reaction mixture contained 3.5 mL of 0.20 M phosphate buffer (pH = 6.8),...

Author(s): Chikezie, P. C., Akuwudike, A. R. and Chikezie, C. M.

September 2013

Induction of thermotolerance through heat acclimation in lablab bean (Dolichos lablab)

The acclimation of plants to moderately high temperature plays an important role in inducing plant tolerance to subsequent lethal temperatures. This study was performed to investigate the effects of heat acclimation and sudden heat stress on the antioxidant and metabolic profile of lablab bean (Dolichos lablab). Following separate pretreatments with heat acclimation (35°C) and NaCl (100 mM), seedlings of lablab...

Author(s): Myrene, R. D’souza and Devaraj, V. R.

September 2013

Determination of ulcer protecting effect of ethanol extract of Gongronema latifolium in rats

Ethanol extract of dietary vegetable, Gongronema latifolium, was evaluated for anti-ulcer activity. The extract was obtained from air-dried, pulverized leaves of the plant following its maceration in ethanol, filteration with Whatman No 1 filter paper and drying at 110°C. Fractionation of the dry crude ethanol extract was stepwisely carried out in with n-hexane, chloroform and ethylacetate, respectively, and...

Author(s): Ezekwe, C. I.