African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Table of Content: 1 May 2012; 11(35)

May 2012

Inflorescence rot disease of date palm caused by Fusarium proliferatum in Southern Iraq

  Date palm is one of the important income sources for many farmers in different parts of several countries, including Iraq, Iran, Saudi Arabia, North Africa etc. Inflorescence rot is a serious disease of date palm which limits its yield. The identification of the causal organism is a key step to tackling this disease, and such studies are very scanty. The objective of this present study was to identify the...

Author(s): Muhammed Abdulrazzaq Hameed

May 2012

Polyphenolics free DNA isolation and optimization of PCR-RAPD for fennel (Foeniculum vulgare Mill.) from mature and young leaves

  Molecular analysis of fennel (Foeniculum vulgare Mill.) strictly relies on high yield, and good quality high molecular weight DNA samples. DNA was isolated from the mature and fresh young tender leaves obtained from various Italian wild populations of fennel. We performed a modified cetyl trimethyl ammonium bromide (CTAB) protocol introducing several modifications such as inclusion of variable percentage...

Author(s): Siva Arumugam Saravanaperumal and Antonietta La Terza

May 2012

Genetic diversity among endangered rare Dalbergia cochinchinensis (Fabaceae) genotypes in Vietnam revealed by random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers

  Dalbergia cochinchinensis is a species of legume in the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Genetic diversity of the 35 genotypes ofD. cochinchinensis species were evaluated by using 52 markers random amplified polymorphic DNA and inter simple sequence repeats (27 RAPD and...

Author(s): Vu Thi Thu Hien and Dinh Thi Phong

May 2012

Identification of different selected genotypes of salinity resistance of each of full-sib Nile tilapia, Blue tilapia and their diallel interspecific hybridization using random amplified polymorphic DNA fingerprinting

  Random amplified polymorphic DNA (RAPD) analysis was applied to identify the three selected genotypes which were defined as; low, medium and high salinity resistance of each of full-sib Nile tilapia (Oreochromis niloticus), Blue tilapia (Oreochromis aureus) and their diallel interspecific hybridization. Six random primers were used to assay polymorphisms among the different selected genotypes within each...

Author(s): Samy Yehya El-Zaeem,

May 2012

Identification of GPD1 gene from yeast via fluorescence differential display-polymerase chain reaction (FDD-PCR)

  The main task of this work was to identify abiotic stress-induced gene(s) from yeast (Saccharomyces cerevisiae) and introduce it to a prokaryotic system to detect its effect on conferring tolerance to salt stress. Six isolates of yeast (S. cerevisiae) were evaluated under salt and osmotic stresses at concentrations of 2 M NaCl and 2 M sorbitol, respectively, in which one isolate was selected as the most...

Author(s): S. Edris, M. H. Z. Mutwakil, J. S. M. Sabir, A. M. Ramadan, N. O. Gadalla, A. M. Shokry, M. A. Al-Kordy, S. E. M. Abo-Aba, F. M. El-Domyati, and A. Bahieldin,

May 2012

Effects of 24-epibrassinolide on photosynthesis of eggplant (Solanum melongena L.) seedlings under salt stress

  In this study, 24-epibrassinolide (EBR), an active brassinosteroid, was exogenously applied to investigate photosynthetic performance of salt-stressed eggplant (Solanum melongena L.). EBR, especially 100 nM, dramatically alleviated growth suppression caused by salt stress. Similarly, the application of 100 nM EBR, the most effective concentration, for salt-stressed plants significantly increased...

Author(s): Xue-Xia Wu, Hai-Dong Ding, Zong-Wen Zhu, Shao-Jun Yang and Ding-Shi Zha,

May 2012

Application of Trichoderma harziunum T22 as a biofertilizer supporting maize growth

  This study was conducted to investigate the effect of Trichoderma harzianum strain (T22) on the growth and development of maize (Zea mays) plants. T. harzianum was applied to the grains in two different treatments. The first one was performed by inoculating the soil with the air dried growth of the tested fungus in three different levels (2, 4 and 6 g kg-1) before sowing. The second...

Author(s): Samia Ageeb Akladious and Salwa Mohamed Abbas

May 2012

Influence of Agrobacterium rhizogenes on induction of hairy roots for enhanced production of artemisinin in Artemisia annua L. plants

  Artemisinin production from plant tissue cultures and induction of hairy roots in vitro have been considered to be a promising alternative, which offer a high degree of genetic stability, grow rapidly and produce the higher spectrum of secondary metabolites than wild type plants. Hairy root cultures developed from infection of different explants of in...

Author(s): Seema Ahlawat, Parul Saxena, Mauji Ram, Pravej Alam, Tazyeen nafis, Anis Mohd and Malik Zainul Abdin

May 2012

Microbial populations during maize storage in Cameroon

  Key microbiota of maize kernels from two farms in Cameroon were characterised at harvest and during five months of drying / storage in firewood kitchens. Moulds populations shifted from Fusarium sp. to Penicillium andAspergillus during drying, and aflatoxigenic moulds were absent. Lactic acid bacteria (Lactobacillus plantarum, Leuconostoc citreum) were only present earlier on,...

Author(s): Su-lin L. Leong, Aziwo T. Niba, Sofia Ny and Matilda Olstorpe

May 2012

Assessment relationship between agro-morphological traits and grain yield in bread wheat genotypes under drought stress condition

  To evaluate the genetic diversity in 140 bread wheat landraces along with six controls (Azar2, Fankang, Trakia, Siosson, Gaspard and Gascogne), an experiment based on randomized complete block design with three replications was carried out under drought stress condition in the Agricultural Research Station of Islamic Azad University, Ardabil, Iran. Analysis of variance indicated that there were...

Author(s): Mehdi habibpour, Mostafa Ahmadizadeh and Hossein Shahbazi

May 2012

Minimizing the hyperhydricity associated with in vitro growth and development of watermelon by modifying the culture conditions

  Hyperhydricity or glassiness is considered as a frequent problem associated with thein vitro growth and development of watermelon (Citrulus lanatus, cv. Giza 1). Explants were cultured on MS (Murashige and Skoog, 1962) medium containing 6-bensyladenine (BA), kinetin (Kin) or thidiazuron (N-phenyl N 1,2,4-chlorophenoxyacetic acid) (TDZ) each applied at 0, 0.25, 0.5, 1, 2 and 4 mg L-1). The...

Author(s): Awatef M. Badr-Elden, Ahmed A. Nower, Ibrahim A. Ibrahim, Mohsen K.H. Ebrahim, and Tamer M. Abd Elaziem

May 2012

BioRadBase: A database for bioremediation of radioactive waste

  Radioactive waste from different sectors such as nuclear energy, health care and food has become a discernible part of our environment. Several dumping methods are in routine practice to dispose radioactive wastes. Huge chemical and energy input in various skillful physico-chemical methods has limited their use while biological methods exploring the potential of micro-organisms could be a promising and...

Author(s): Reena, Manash Chandra Majhi, Arvind Kumar Arya, Rita Kumar and Anil Kumar

May 2012

Antioxidant and anti-browning activities of Mentha suaveolens extracts

  The inhibition of enzymatic browning and oxidation is still an important challenge in food processing. This study was designed to examine in vitro the inhibition of enzymatic browning in apple and the antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferrous ion chelating activity by Mentha suaveolensphenol, methanol and aqueous extracts. The use of various extracts in...

Author(s): Madiha Bichra, Cherkaoui El modafar, Es-saddik El boustani and Fatiha Benkhalti

May 2012

Haematological and serum biochemical indices of pre-pubertal male rabbits fed with graded level of blood-wild sunflower forage meal mixture

  The effects of feeding graded levels of blood wild sunflower forage meal mixture (BWSFM) on haematological and serum biochemical parameters in rabbit were studied. Thirty-two (32) cross-bred (New Zealand-white X Chinchilla) male weaner rabbits aged between 6 and 9 weeks were randomly divided after weight balancing into four groups of eight rabbits per group and each rabbit served as replicate...

Author(s): Ajayi, Ayodeji F. and Raji, Y.

May 2012

Induction of protective immunity against toxoplasmosis in mice by immunization with a plasmid encoding Toxoplama gondii ROP1 gene

  Toxoplasma gondii is an obligate intracellular protozoan that is a causative agent of toxoplasmosis, a disease which may result in a spectrum of consequences. It has been shown that DNA vaccine can be effective in partial protection against this parasite. In the present work, a single DNA vaccine containing ROP1 was evaluated against T. gondii infection in Balb/c mice. To enhance the immune...

Author(s): Z. Eslamirad, A. Dalimi, F. Ghaffarifar, Z. Sharifi and A. Zavaran Hosseini

May 2012

Length based seasonal growth of the garfish, Belone belone (Linnaeus, 1761) (Belonidae), in the southeast Black Sea

  The seasonal growth pattern of the garfish, Belone belone, L., 1761 was studied in the southeastern Black Sea between October 2009 and March 2011. The seasonal von Bertalanffy growth (VBG) parameters which were computed from monthly length frequency distributions, were estimated as L∞ = 41.16 cm total length (TL), K = 0.728 yr-1, to = -0.51 year for female and...

Author(s): Ferhat Kalayci and Tuncay Yeşilçiçek

May 2012

Localization of immunodominant linear B-cell epitopes of Vibrio mimicus outer membrane protein U (OmpU)

  Outer membrane protein U (OmpU), an adhesion protein of Vibrio mimicus, is a good antigen, but its epitopes are still unclear. In order to locate the epitopes of OmpU protein, epitope prediction was performed using the amino acid sequence of OmpU protein of V. mimicus HX4 strain that was isolated from the diseased crass carp in Anhui province of China. The secondary structure, flexible regions,...

Author(s): Ping-Ping Wang, Jun-Yu Cen, Hong-Mei Liu, Jin-Nian Li and Ming Zhang

May 2012

Optimizing the acceleration of biotechnology innovation in government programs

  Science biotechnology has been attributed a superior platform in Malaysian government plan for wealth creation in the 9th Malaysian plan and policy of Malaysia’s science and technology in 21st century; it has been accepted and categorized as a complicated emerging issue to illustrate high prominence combined with restricted knowledge in environment. Researchers emphasize on the importance of...

Author(s): Hadi Farid, Abu Daud Silong, Ismi Arif Ismail and Saroje Kumar Sarkar

May 2012

The effect of guar meal (germ fraction) and β-mannanase enzyme on growth performance and plasma lipids in broiler chickens

  A completely randomized design experiment was conducted to assess the effect of β-mannanase enzyme on a commercial broiler chickens strain fed with different levels of guar meal germ fraction (GM). Each treatment was assigned to 4 replicate for a total of 24 pens, consisting of 15 chicks per pen, in randomized complete block design. Experimental groups include: control diet without GM, low...

Author(s): Mohayayee, Mohammad and Karimi, Kazem