African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 20 August, 2014; 13(34)

August 2014

Shoot regeneration, biochemical, molecular and phytochemical investigation of Arum palaestinum Boiss

Arum palaestinum Boiss. populations are in danger of extinction in the wild. Thus, there is a need to establish a reliable strategy for multiplying this valuable medicinal plant. In the present study, seeds and tissue culture of A. palaestinum were subjected to biochemical, molecular and phytochemical analysis. Obtained results indicated that the best medium for shoots proliferation was Murashige and Skoog (MS) medium...

Author(s): Mai M. Farid, Sameh R. Hussein, Lamiaa F. Ibrahim,  Mohammed A. El Desouky, Amr M. Elsayed and Mahmoud M. Saker

August 2014

Molecular identification of phosphate solubilizing bacterium (Alcaligenes faecalis) and its interaction effect with Bradyrhizobium japonicum on growth and yield of soybean (Glycine max L.)

A phosphate solubilizing bacterium was isolated from the rhizosphere soil of upland rice and identified by 16S rRNA gene sequencing. The gene sequence showed 99% homology with Alcaligenes faecalis. Based on the gene sequence homology, it was identified as A. faecalis. Interaction effect of this bacterium on growth and yield of soybean was studied under glass house conditions by inoculating the bacterium either alone or...

Author(s): Nandini, K., Preethi, U. and Earanna, N.,

August 2014

Polyclonal antibodies of Ganoderma boninense isolated from Malaysian oil palm for detection of basal stem rot disease

Basal stem rot (BSR) disease caused by the fungus, Ganoderma boninense has become a serious threat to the oil palm industry, especially in Southeast Asia (SEA). A highly selective and sensitive diagnostic tool for BSR is extremely required for early detection, and thus, development of immunological test using enzyme-linked immunosorbent assay-polyclonal antibody (ELISA-PAb) was evaluated. Results indicate that ELISA-PAb...

Author(s): Madihah, A. Z., Idris, A. S. and Rafidah, A. R.

August 2014

Molecular characterization of cultivated cowpea (Vigna unguiculata L. Walp) using simple sequence repeats markers

Forty eight accessions of cultivated cowpea were assessed using 12 simple sequence repeat (SSR) markers. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram constructed revealed three main clusters when truncated at 65% similarity coefficient. All accessions showed high genetic variation except four which are genetically similar at 100% similarity coefficient. A two dimensional principal coordinate...

Author(s): Ogunkanmi, L. A., Ogundipe, O. T. and Fatokun, C. A.

August 2014

Use of spent compost in the cultivation of Agaricus blazei

Two compost formulations, based on Braquiaria straw (Brachiaria sp.), a conventional one and a spent one, were tested in the cultivation of ABL 99/30 and ABL 04/49 strains of Agaricus blazei. The experimental design was in a completely randomized factorial scheme with four treatments (two strains of A. blazei x two types of compost) and 30 repetitions. Each experimental unit consisted of a box with 10 to 10.5 kg of...

Author(s): Gabriel Madoglio Favara, Ceci Sales-Campos, Marli Teixeira De Almeida Minhoni, Otavio Augusto Alves Pessoto Siqueira and Meire Cristina Nogueira De Andrade

August 2014

Field efficacy of inorganic carrier based formulations of Serratia entomophila AB2 in Sesamum indicum var. Kanak

Serratia entomophila is a well-known bacterium of agricultural importance for its nutrient (P and Zn) solubilization, plant growth promoter (IAA) production, antifungal activity and larvaecidal activity against coleopteran and lepidopteron pest. In the present study, an attempt was taken to reduce the use of chemical pesticide and fertilizer by using two inorganic carriers (talcum powder and vermiculite) based...

Author(s): Pritam Chattopadhyay, Nilima Karmakar, Sandipan Chatterjee and Sukanta K. Sen

August 2014

Crude oil degrading potential of Pennisetum glaucum (L.) R. Br

Pollution by crude oil and its products is one of the most prevalent environmental problems that cause greenhouse effects and global warming. The crude oil-degrading potentials of Pennisetum glaucum was investigated using 0.2, 0.9, 5.0 and 6.0% v/w concentrations of crude oil, which were employed to pollute soil planted with the seeds of the plant. These treatments were repeated in soil without seeds and the control had...

Author(s): Nwadinigwe Alfreda Ogochukwu and Obi-Amadi Achuna

August 2014

Growth response of region specific Rhizobium strains isolated from Arachis hypogea and Vigna radiata to different environmental variables

Six different strains isolated from Vigna radiata (IARI-1 UU-2, UU-4, UU-7, UU-10 and UU-13) and seven strains from Arachis hypogea (IARI-16, UU-17, UU-18, UU-19, UU-20, UU-21 and UU-22) were selected to assess their capability to tolerate environmental variables like pH, temperature, salinity (NaCl), iron (Fe), phosphate (K2HPO4), and nitrate (NaNO3). The bacteria under investigation expressed noticeable ability to...

Author(s): Santosh Kumar Sethi and Siba Prasad Adhikary

August 2014

Antimicrobial activity of Streptomyces sp. isolated from the gulf of Aqaba-Jordan and screening for NRPS, PKS-I, and PKS-II genes

Forty-nine Streptomyces isolates were recovered from sediment samples in the gulf of Aqaba/Jordan. All isolates were tested for antimicrobial activity against Gram positive bacteria, Gram negative bacteria, and yeast. Twenty eight Streptomyces isolates were active against at least one of the tested strains. The majority of the isolates showed activity against Gram positive bacteria: Streptomyces aureus (89%),...

Author(s): Fayza Kouadri, Amal Al-Aboudi and Hala Khyami-Horani

August 2014

Moringa oleifera leaf extract potentiates anti-pseudomonal activity of ciprofloxacin

The aim of this study was to evaluate the in vitro antimicrobial interaction between the ethanol leaf extract of Moringa oleifera (MO), which is used in Nigeria as a dietary supplement, and ciprofloxacin (Cp), a flouroquinolone antibiotic. Preliminary antimicrobial screening of the ethanol extract of M. oleifera and ciprofloxacin was determined in vitro using the agar dilution method. The antimicrobial interaction...

Author(s): David B. Okechukwu, Franklin C. Kenechukwu and Chioma A. Obidigbo

August 2014

Two-dimensional profiling of Xanthomonas campestris pv. viticola proteins extracted by four different methods

An efficient method for protein extraction is a prerequisite for the successful implementation of proteomics, which is being used as a tool in the study of the interaction between plants and phytopathogens. With the objective to optimize a method of protein extraction for proteomic analysis of the phytobacterium Xanthomonas campestris pv. viticola, the efficiency of four methodologies were compared, based on the...

Author(s): Myrzânia de Lira Guerra, Carolina Barbosa Malafaia, Túlio Diego da Silva, Rosa de Lima Ramos Mariano, Márcia Vanusa da Silva and Elineide Barbosa de Souza

August 2014

Multidrug-resistant hepatocellular carcinoma cells are enriched for CD133+ subpopulation through activation of TGF-β1/Smad3 pathway

Chemotherapy is a main treatment for cancer, while multidrug-resistance is the main reason for chemotherapy failure, and tumor relapse and metastasis. Cancer stem cells or cancer stem-like cells (CSCs) are a small subset of cancer cells, which may be inherently resistant to the cytotoxic effect of chemotherapy. Some studies suggest that CSCs could be enriched by chemotherapy. However, the mechanism of chemotherapy...

Author(s): Wei Yan, Fen Lin, Ting Wen, Zhongcai Liu, Suqiong Lin, and Guoyang Wu