February 2016
Antiproliferative activity of protein extracts from the black clam (Chione fluctifraga) on human cervical and breast cancer cell lines
The wide diversity of the marine environment has been an important resource for the discovery of new bioactive agents from marine organisms. The aim of this study was to obtain protein extracts from the clam Chione fluctifraga and determine its antiproliferative activity against cervical and breast cancer cells. The extracts were obtained by ammonium sulfate fractionation, gel filtration and ion exchange chromatography....
February 2016
Toxicity effects of ethanol extract of Simarouba versicolor on reproductive parameters in female Wistar rats
Simarouba versicolor is popularly known as pau-paraiba. It belongs to the Simaroubaceae family and is found in the Northeast of São Paulo and in some parts of the states of Pará and Mato Grosso do Sul. It is known that this and other species from this genus have several activities: insecticides, anti-inflammatory, antitumor, anthelmintic, among others. The effects of ethanolic extract of the bark of S....
February 2016
Codon usage bias analysis for the coding sequences of Camellia sinensis and Brassica campestris
Codon usage bias plays an important role in the regulation of gene expression. A couple of measures are widely used to quantify the codon usage in genes. On the other hand, no quantitative endeavour has been made to compare the pattern of codon usage diversity within and between different genes of Camellia sinensis and Brassica campestris. Nucleotide composition and its relationship with codon usage bias were analyzed....
February 2016
Identification of accelerated evolution in the metalloproteinase domain of snake venom metalloproteinase sequences (SVMPs) through comparative analysis
Computational protein sequence analysis is one of the most important tools used for understanding the evolution of closely related proteins sequences including snake venom metalloproteinase sequences (SVMPs) which give valuable information regarding genetic variations. The fundamental objective of the present study is to screen the evolution distributed in metalloproteinase domain regions of protein sequences among...
February 2016
Genetic variability of sorghum landraces from lower Eastern Kenya based on simple sequence repeats (SSRs) markers
The aim of this study was to estimate the genetic variability of sorghum landraces grown in lower eastern Kenya based on simple sequence repeats (SSRs) markers. A total of 44 landraces obtained from the farmers and four improved varieties were analyzed using 20 SSR markers. All markers were polymorphic with polymorphism information content (PIC) value ranging from 0.04 to 0.81 with a mean of 0.49. Heterozygosity ranged...
February 2016
Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews)
As an alternative to conventional lighting systems, light emitting diode (LED) has been demonstrated to be an artificial flexible lighting source for commercial micropropagation. The objective of this study was to determine the effects of different LED light quality on in vitro shoot proliferation and growth of Vanilla planifolia. To evaluate shoot proliferation, axillary bud cuttings (3 to 5 mm in diameter) of V....
February 2016
Detection of metallo-beta-lactamase producing Pseudomonas aeruginosa using a modified IMP-lysate assay
Since the increasing prevalence of carbapenem-resistant Pseudomonas aeruginosa spp., accurate detection of metallo-β-lactamase (MBL) such as blaVIM type enzyme producing isolates became very important. However, phenotypic MBL detection methods previously reported are not highly sensitive or highly specific. This study aimed to evaluate the performance of a modified IMP-lysate test, the double-disk-synergy-test...
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