African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12421

Table of Content: 28 August, 2012; 11(69)

August 2012

Microsatellite markers reveal Cameroon’s wild oil palm population as a possible solution to broaden the genetic base in the Indonesia-Malaysia oil palm breeding programs

Intensive breeding from a narrow genetic base of the South East Asian oil palm industry has led to limited variation within elite breeding population thereby reducing the rate of future breeding progress. The use of wild genotypes are known to broaden the genetic base of breeding populations because of their high rate of heterozygosity. Historical evidence show that Cameroon has supplied wild genetic material to the...

Author(s): Walter Ajambang, Sudarsono, Dwi Asmono and Nurita Toruan

August 2012

Quantification of markers of antimalarial drug resistance from an area of high malaria transmission: Comparing frequency with prevalence

Molecular monitoring of markers of antimalarial drug resistance offers an affordable alternative to the in vivo method for the detection of resistance, and has the potential to guide public health policy in a timely manner. However, the optimal way of analyzing and reporting these data, particularly those emanating from areas of moderate to high malaria transmission, has never been fully explored or agreed...

Author(s): Allen Malisa, Richard Pearce, Ben Mutayoba, Salim Abdullah, Hassan Mshinda, Patrick Kachur, Peter Bloland and Cally Roper

August 2012

Utility of adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] simple sequence repeat (SSR) markers in genetic analysis of mungbean and related Vigna spp.

In the present study, 78 mapped simple sequence repeat (SSR) markers representing 11 linkage groups of adzuki bean were evaluated for transferability to mungbean and relatedVigna spp. 41 markers amplified characteristic bands in at least one Vigna species. The transferability percentage across the genotypes ranged from 60.97 to 92.6% with 87.8% in Vigna radiata and Vigna mungo, 62.2%...

Author(s): H. K. Dikshit, D. Singh, Akanksha Singh, Neelu Jain, Jyoti Kumari and T. R. Sharma

August 2012

In silico comparative analysis of EST-SSRs in three cotton genomes

In this study, expressed sequence tags- simple sequence repeat (EST-SSRs) were surveyed in three cotton genomes (Gossypium arboreum, Ga; Gossypium raimondii, Gr and Gossypium hirsutum, Gh). The frequency of EST-SSRs was highest in Gr, and motif type for hexanucleotide was obviously abundant in Gr. Trinucleotide repeats were the most abundant motif; AT and AG, AAG and ATC were the most frequent motifs for...

Author(s): Daojun Yuan#, Shaoguang Liang#, Zhongxu Lin and Xianlong Zhang

August 2012

Consequences of diverse use of nitrogen sources on grain yield, grain quality and growth attributes of hybrid maize (Zea mays L.)

A two year field experiment was conducted to check the consequences of diverse use of nitrogen sources on grain yield, grain quality and growth attributes of hybrid maize (Zea mays L.) at the Agronomic Research Area, University of Agriculture, Faisalabad during Autumn 2008 and 2009. Experiments were laid out in a randomized complete block design with factorial arrangement comprising 3 replications...

Author(s): Muhammad Waseem, Asghar Ali, Muhammad Tahir, Muhammad Naeem, Muhammad Ayub, Asif Iqbal, Atta Ullah Mohsin, Haseeb-ur-Rehman, Muqarrab Ali, Azhar Ghaffari, M.Aqeel Sarwar and Abdul Khaliq

August 2012

Over expression of Zmda1-1 gene increases seed mass of corn

Genetic engineering of seed size and increasing biomass in crop plants has an important significant contribution to the world. Arabidopsis DA1 is one of the key factors that negatively control seed and organ size by restricting the period of cell proliferation, and the mutant of Arabidopsis DA1, da1-1 (DA1R358K) can dramatically increase the size of seed. However, it is not clear whether overexpression of Zmda1-1, the...

Author(s): Xiaohan Wang, Binlin Liu, Conglin Huang, Xiuhai Zhang, Chang Luo, Xi Cheng, Rong Yu and Zhongyi Wu,

August 2012

Selection of superior wheat genotypes against end-season drought of Ardabil in the presence of humic fertilizer by utilization of multivariate statistics

Irrigated wheat in cold regions of Iran is faced with the end drought stress. Humic substances, as natural biological origin fertilizers have mitigation activity on plants facing the biotic and abiotic stresses. This experiment was conducted by a split plot on the basis of completely randomized block design (RB) in Ardabil in 2008 to 2009 farmer year. Main factor was stressed and non stressed...

Author(s): Vahid Mollasadeghi, Mostafa Valizadeh, Reza Shahryari and Ali Akbar Imani

August 2012

Assessment of compost for suppression of Fusarium oxysporum and improving Zea mays and Hibiscus sabdarriffa resistance to wilt diseases

The present research was conducted to evaluate the compost effectiveness on Zea mays and Hibiscus sabdarriffa under Fusarium wilt disease. Compost physical, chemical and biological characters were monitored weekly during the ripening process. Both coliform and nematode were tested. Finally, the effect of compost on pathogenicFusarium was examined within Z....

Author(s): W. M. Salem, W. F. Sayed, H. Abdel-Fatah and H. H. Neamat

August 2012

Isolation of alkaline protease from Bacillus subtilis AKRS3

This research study was mainly focused on phenotypic, biochemical characterization, 16s rRNA sequence based species level identification of isolate and determination of the higher production of alkaline protease through optimization study (carbon, nitrogen, incubation period, temperature, pH and sodium chloride concentration), production by submerged fermentation and analytical studies (protease assay, protein and...

Author(s): Krishnan Ravishankar, Muthusamy Ashok kumar and K. Saravanan

August 2012

Influence of starch source in the required hydrolysis time for the production of maltodextrins with different dextrose equivalent

The starches were statistically different (p < 0.05) in their protein, ash, fat, phosphorus, amylose and amylopectin content. The amylose/amylopectin ratios in corn, potato and rice starches were 0.389, 0.282 and 0.220, respectively. The phosphorus content in the same order of starches was: 0.15 ± 0.01; 0.80 ± 0.02 and 0.95 ± 0.02 g/kg, respectively. The chemical composition of the different...

Author(s): José Luis Montañez Soto, Luis Medina García, José Venegas González, Aurea Bernardino Nicanor and Leopoldo González Cruz

August 2012

Monitoring of a novel bacterium, Lactobacillus thermotolerans, in chicken intestine by fluorescence in situ hybridization (FISH)

We successfully established fluorescence in situ hybridization (FISH) method for specific detection and enumeration of a novel bacterium, Lactobacillus thermotolerans, in chicken feces. The specific FISH probes were designed based on the L. thermotolerans16S rRNA gene sequences, and these sequences were compared to those of all available 16S rRNA genes in the GenBank...

Author(s): Abu Sadeque Md. Selim and Atsushi Yokota