August 2012
Microsatellite markers reveal Cameroon’s wild oil palm population as a possible solution to broaden the genetic base in the Indonesia-Malaysia oil palm breeding programs
Intensive breeding from a narrow genetic base of the South East Asian oil palm industry has led to limited variation within elite breeding population thereby reducing the rate of future breeding progress. The use of wild genotypes are known to broaden the genetic base of breeding populations because of their high rate of heterozygosity. Historical evidence show that Cameroon has supplied wild genetic material to the...
August 2012
Quantification of markers of antimalarial drug resistance from an area of high malaria transmission: Comparing frequency with prevalence
Molecular monitoring of markers of antimalarial drug resistance offers an affordable alternative to the in vivo method for the detection of resistance, and has the potential to guide public health policy in a timely manner. However, the optimal way of analyzing and reporting these data, particularly those emanating from areas of moderate to high malaria transmission, has never been fully explored or agreed...
August 2012
Utility of adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] simple sequence repeat (SSR) markers in genetic analysis of mungbean and related Vigna spp.
In the present study, 78 mapped simple sequence repeat (SSR) markers representing 11 linkage groups of adzuki bean were evaluated for transferability to mungbean and relatedVigna spp. 41 markers amplified characteristic bands in at least one Vigna species. The transferability percentage across the genotypes ranged from 60.97 to 92.6% with 87.8% in Vigna radiata and Vigna mungo, 62.2%...
August 2012
In silico comparative analysis of EST-SSRs in three cotton genomes
In this study, expressed sequence tags- simple sequence repeat (EST-SSRs) were surveyed in three cotton genomes (Gossypium arboreum, Ga; Gossypium raimondii, Gr and Gossypium hirsutum, Gh). The frequency of EST-SSRs was highest in Gr, and motif type for hexanucleotide was obviously abundant in Gr. Trinucleotide repeats were the most abundant motif; AT and AG, AAG and ATC were the most frequent motifs for...
August 2012
Consequences of diverse use of nitrogen sources on grain yield, grain quality and growth attributes of hybrid maize (Zea mays L.)
A two year field experiment was conducted to check the consequences of diverse use of nitrogen sources on grain yield, grain quality and growth attributes of hybrid maize (Zea mays L.) at the Agronomic Research Area, University of Agriculture, Faisalabad during Autumn 2008 and 2009. Experiments were laid out in a randomized complete block design with factorial arrangement comprising 3 replications...
August 2012
Over expression of Zmda1-1 gene increases seed mass of corn
Genetic engineering of seed size and increasing biomass in crop plants has an important significant contribution to the world. Arabidopsis DA1 is one of the key factors that negatively control seed and organ size by restricting the period of cell proliferation, and the mutant of Arabidopsis DA1, da1-1 (DA1R358K) can dramatically increase the size of seed. However, it is not clear whether overexpression of Zmda1-1, the...
August 2012
Selection of superior wheat genotypes against end-season drought of Ardabil in the presence of humic fertilizer by utilization of multivariate statistics
Irrigated wheat in cold regions of Iran is faced with the end drought stress. Humic substances, as natural biological origin fertilizers have mitigation activity on plants facing the biotic and abiotic stresses. This experiment was conducted by a split plot on the basis of completely randomized block design (RB) in Ardabil in 2008 to 2009 farmer year. Main factor was stressed and non stressed...
August 2012
Assessment of compost for suppression of Fusarium oxysporum and improving Zea mays and Hibiscus sabdarriffa resistance to wilt diseases
The present research was conducted to evaluate the compost effectiveness on Zea mays and Hibiscus sabdarriffa under Fusarium wilt disease. Compost physical, chemical and biological characters were monitored weekly during the ripening process. Both coliform and nematode were tested. Finally, the effect of compost on pathogenicFusarium was examined within Z....
August 2012
Isolation of alkaline protease from Bacillus subtilis AKRS3
This research study was mainly focused on phenotypic, biochemical characterization, 16s rRNA sequence based species level identification of isolate and determination of the higher production of alkaline protease through optimization study (carbon, nitrogen, incubation period, temperature, pH and sodium chloride concentration), production by submerged fermentation and analytical studies (protease assay, protein and...
August 2012
Influence of starch source in the required hydrolysis time for the production of maltodextrins with different dextrose equivalent
The starches were statistically different (p < 0.05) in their protein, ash, fat, phosphorus, amylose and amylopectin content. The amylose/amylopectin ratios in corn, potato and rice starches were 0.389, 0.282 and 0.220, respectively. The phosphorus content in the same order of starches was: 0.15 ± 0.01; 0.80 ± 0.02 and 0.95 ± 0.02 g/kg, respectively. The chemical composition of the different...
August 2012
Monitoring of a novel bacterium, Lactobacillus thermotolerans, in chicken intestine by fluorescence in situ hybridization (FISH)
We successfully established fluorescence in situ hybridization (FISH) method for specific detection and enumeration of a novel bacterium, Lactobacillus thermotolerans, in chicken feces. The specific FISH probes were designed based on the L. thermotolerans16S rRNA gene sequences, and these sequences were compared to those of all available 16S rRNA genes in the GenBank...
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