African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12470

Table of Content: 29 August, 2018; 17(35)

August 2018

Simple and rapid detached leaf technique for screening common beans (Phaseolus vulgarise L.) in vitro against angular leaf spot (Pseudocercospora griseola) disease

A rapid and reliable detached-leaf technique was developed for screening common bean (Phaseolus vulgaris) genotypes against angular leaf spot (ALS) disease caused by Pseudocercospora griseola. It is helpful to differentiate between susceptible and resistance common bean genotypes. Detached leaves of common bean tested genotypes were inoculated by immersing them into a spore suspension followed by placing into petri...

Author(s): Yayis Rezene, Kassahun Tesfaye, Clare Mukankusi, Esther Arunga and Paul Gepts  

August 2018

Isolation and regeneration of protoplasts from leaf explants of Rhyncholaelia digbyana

A protocol for the isolation and regeneration of protoplasts from leaf explant of Rhyncholaelia digbyana is presented. The protoplasts were isolated using hemicellulase enzymes at 1.5, 2.25, and 3% (w/v), pectinase at 0.5 and 0.75% (w/v) and cellulase at 1 and 2% (w/v). Protoplast counting was carried out with a Neubauer camera and an optical microscope at 40X, and viability was determined with Evans blue dye at 0.025%...

Author(s): Luis Alberto Mota-Narváez, Gilbert José Herrera-Cool,Teresa del Rosario Ayora-Talavera, Nancy Santana-Buzzy, and Guadalupe López-Puc  

August 2018

Detection of genetically modified DNA in processed maize and soybean products in Nigeria

Cultivation and commercial utilization of genetically modified (GM) crops has significantly increased in many parts of the world and particularly in developing countries where food security is a challenge. Despite stringent regulations requiring that food made from GM foods should be properly labelled, evidence of unlabelled foods made from GM crops sold in local markets in many of these countries is increasing. This...

Author(s): Andrew Chibuzor Iloh, Paul Chidozie Onyenekwe and Opeolu Oyejide Ojo,  

August 2018

Preservative potentials of essential oils of three Nigerian spices in mixed fruit juice and their antioxidant capacity

The study was conducted to evaluate the preservative potentials of essential oils (EOs) of Piper guineense, Xylopica aethiopica and Tetrapleura tetraptera in mixed fruit juice and determine their antioxidant capacity. The preservative effect at varying concentrations was evaluated using S. cerevisiae, S. aureus and B. cereus as test isolates. The antioxidant properties were determined using 1,1-...

Author(s): Ogueke Chika C., Nnadi Nkechi B., Owuamanam Clifford I., Ojukwu Moses, Nwachukwu Ikenna N., Ibeabuchi Chidi J and Bede Evelyn N.